LOCUS       sequence-130919-         974 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pFOSGE_CUS1H02
FEATURES             Location/Qualifiers
     source          1..974
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    71..95
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(687..786)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(803..822)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(804..822)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(827..843)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(827..843)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(945..964)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 tatgatttta ttttgactga tagtgacctg ttcgttgcaa caaattgatg agcaatgctt
       61 ttttataatg ccaactttgt acaaaaaagc aggcttcatg gacgaaatcc tctttcccac
      121 gcagcaaaaa agcaactccc taagcgacgg cgacgatgtc gacctgaaat tcttccagtc
      181 ggcctccggc gagcggaagg acagcgacac ctcggatccg ggaaacgatg cggatcgtga
      241 tggcaaggat gcggatgggg acaacgacaa caagaacacg gacggagatg gtgactctgg
      301 cgagccggcg cacaaaaagc tcaagacgaa gaaggaactg gaggaggagg agcgcgaacg
      361 aatgcaggtt ctcgtttcca actttacgga agaacagctg gatcgctacg aaatgtatcg
      421 tcgctctgcc tttcccaagg ccgccgtcaa gcgtctaatg caaactatca ccggctgttc
      481 cgtgtcccag aatgttgtga tagccatgtc cggcattgcg aaggtcttcg tcggcgaggt
      541 tgtggaggaa gccctcgacg tgatggaggc ccaaggtgaa tccggtgccc tgcagcccaa
      601 attcatacga gaggcagtgc gacgactgag gaccaaggat cggatgccca taggcagata
      661 ccagcagccc tatttcagac tgaacgaccc agctttcttg tacaaagttg gcattataag
      721 aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat
      781 tatttgccat ccagctgata tcccctatag tgagtcgtat tacatggtca tagctgtttc
      841 ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat
      901 atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag
      961 ccatattcaa cggg
//
