LOCUS       sequence-13092-e        3885 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 3885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1433-HQ858735
FEATURES             Location/Qualifiers
     source          1..3885
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             112..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(1442..1541)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1558..1577)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1559..1577)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1582..1598)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1582..1598)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1700..1719)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1711..2520
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1778..1797)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2667..3255
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3156..3175
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3409..3426
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3585..3612)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3704..3790)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3839..3861
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3853..3870
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3854..3870
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc cttgtttaac
      121 tttaagaagg agcccttcac catggcgcag aatgatcacc cggctgcctt accgccgcgc
      181 gatgacacgg tggatagctt cctgtcaaga gaggtcctcc gctccgaggt ggggctggat
      241 gccaggcgcg ccgggctcgt cgaaatgctg gagggcgaag agcacagcag cattttcagc
      301 gacgaatccg aggatgacga tgatggcggg catagcatag ccgtaacggt gaagtcggcg
      361 cggcggtccg agagaagggc tcggagagtg agggcagcga tgaagacggc caagtgttcc
      421 agcagcatga agcccgttgg agcgtcctcc acaaggagga aacggttgaa gggttgcctg
      481 actcctctcg ggtgcttgta caagatgacc ggcccgaggc ttctgacggc ggaacaagaa
      541 gtcgaattct cagaaggcat tcaggacctt ctgaaactgg aagccatcca aaaggaggtt
      601 gcgcagtata atgctggtga accaacgttt tcccagtggg cggcagcagc cgggaccgat
      661 gagaacactc tgcggagacg cctgaatcat ggagtctact gcaagaacag gatggtgaaa
      721 tctaacgtgc ggcttgtaat ctcgattgca aaagaacacg aaggccccgg gatggagttc
      781 tctgatctta ttcaggatgg aatgcaaggc ctgataagag gcgcggaaaa gttcgacgct
      841 tccaagggtt ttagattctc cacttactct cactggtgga ttaaacaagc aatacgcaag
      901 tccgttttag aacaaaccca gataatccgc ctgccggcac acatggctga agcgagttcc
      961 cgagtgaagg aatgctgccg gcgactcggc cgtgagctga aacggctccc cagcaacgaa
     1021 gagatcgcgg tggacactgg catgacgatc cggcgggtgg aggcggccat gagcctcccg
     1081 aaatacagcg tgtccttcac gagcaaggtc gggtgcacgg acgtcacgta ccaggagatc
     1141 atgccggaca cgagcgccga gacggcggag gagacgctgc accggtggct gatgaagaag
     1201 gacgtgaaca cggcgctgga cagcctgagc ccgcgcgaga agcaggtgat gcggtaccgg
     1261 ttcggcatcg agggcgggcg ggccaggacc ctgcacgaca tcgggcagct catgggggtc
     1321 agccgggagc ggatccggca gatcgagctg ggcgcgttcc ggaagctgcg gagcaagaac
     1381 aggttgcagt cgctgcagca ctacctgcgg ccggcggaga gctggaaggg tgggcgcgcc
     1441 gacccagctt tcttgtacaa agttggcatt ataagaaagc attgcttatc aatttgttgc
     1501 aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc tgatatcccc
     1561 tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa
     1621 aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct
     1681 gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg
     1741 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat
     1801 gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg
     1861 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta
     1921 aactggctga cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat
     1981 gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa
     2041 tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat
     2101 tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg
     2161 caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc
     2221 tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca
     2281 gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata
     2341 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta
     2401 tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt
     2461 attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa
     2521 tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc
     2581 gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac
     2641 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     2701 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     2761 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     2821 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     2881 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     2941 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     3001 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcat
     3061 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     3121 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     3181 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     3241 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     3301 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     3361 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     3421 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     3481 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     3541 attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca
     3601 ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc
     3661 ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga
     3721 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc
     3781 gttttatttg atgcctggca gttccctact ctcgcgttaa cgctagcatg gatgttttcc
     3841 cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggccc
//
