LOCUS       sequence-13094-e        3369 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1435-HQ858737
FEATURES             Location/Qualifiers
     source          1..3369
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             112..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(926..1025)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1042..1061)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1043..1061)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1066..1082)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1066..1082)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1184..1203)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1195..2004
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1262..1281)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2151..2739
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2640..2659
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2893..2910
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3069..3096)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3188..3274)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3323..3345
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3337..3354
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3338..3354
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc cttgtttaac
      121 tttaagaagg agcccttcac catggggcgt ggaaaagtag agctgaagaa gattgagaat
      181 ccaacaaacc gccaagttac cttctccaag aggcggatgg ggttgttcaa gaaggcaaat
      241 gagctagcca ttctttgtga tgcgcaaatc ggagtcatca tattctctgg cagtggcagg
      301 atgtacgagt actccagccc tccatggaga atagcaagcg tctttgacag gtacctgaaa
      361 gcccctagca cccgtttcga ggagatggac atccagcaga aaatcgtcca ggagatgact
      421 aggatgaagg atgagaggaa cagactgagg atgatcatgg cgcagtacat ggcggaggac
      481 ctggcatcgt tctccgcgca ggatctgagc aaccttgagc agcaaatcga gttctcgctg
      541 tacaaagttc gcctcaggaa gcaagagcta ctcgaccagc agctgctcga gatccaccaa
      601 agggagatgc atatgccagc agagcagggc ggctacctgt gcctcatgaa cccggctgcg
      661 gctattgcta gcgggcagca ccagcaggct ggcgagatgg tgggaattaa cccgaggccg
      721 ttcccgtggt gggacgtggg agccagtggc agtggcagtg gcagccagag ccagcagcag
      781 ctgctgcacg gccgggacgc cgcggagtcg tcgatgacgg cgctcgggct gtcgccgcag
      841 ctgcatgggt acaggctcca gccgcggcag cccaacctgc agcaggacgc cgacatccat
      901 ggctggctga agggtgggcg cgccgaccca gctttcttgt acaaagttgg cattataaga
      961 aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt
     1021 atttgccatc cagctgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc
     1081 tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata
     1141 tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc
     1201 catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc tgatttatat
     1261 gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgcttgtat
     1321 gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat
     1381 gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc
     1441 aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat ccccggaaaa
     1501 acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg
     1561 gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat
     1621 cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt
     1681 gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa
     1741 cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt
     1801 atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac
     1861 cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag
     1921 aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat
     1981 ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc
     2041 attacgctga cttgacggga cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac
     2101 gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     2161 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     2221 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     2281 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     2341 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     2401 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     2461 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     2521 ctgagatacc tacagcgtga gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg
     2581 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     2641 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     2701 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     2761 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     2821 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     2881 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     2941 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     3001 aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga agagtttgta
     3061 gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttt
     3121 atggcgggcg tcctgcccgc caccctccgg gccgttgctt cacaacgttc aaatccgctc
     3181 ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg
     3241 cccagtcttc cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgcg
     3301 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaag
     3361 ctcgggccc
//
