LOCUS       sequence-130959-         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  express in mammalian cells for unnatural amino acid incorporation 
            into VAMP2-GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Vreja IC, Nikic I, Gottfert F, Bates M, Krohnert K, Outeiro TF, Hell
            SW, Lemke EA, Rizzoli SO
  TITLE     Super-resolution Microscopy of Clickable Amino Acids Reveals the 
            Effects of Fluorescent Protein Tagging on Protein Assemblies.
  JOURNAL   ACS Nano. 2015 Nov 24;9(11):11034-41. doi: 10.1021/acsnano.5b04434. 
            Epub 2015 Oct 28.
  PUBMED    26498474
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acsnano.5b04434"; journalName: "ACS Nano"; date: 
            "2015-11-24- 24"; volume: "9"; issue: "11"; pages: "11034-41"
COMMENT     Sequence Label: pEGFP-N1 VAMP2 R125TAG
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      430..439
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(481..502)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(742..761)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 accgtcagat ccgctagcgc taccggactc agatctatgt cggctaccgc tgccaccgtc
       61 ccgcctgccg ccccggccgg cgagggtggc ccccctgcac ctcctccaaa tcttactagt
      121 aacaggagac tgcagcagac ccaggcccag gtggatgagg tggtggacat catgagggtg
      181 aatgtggaca aggtcctgga gcgggaccag aagttgtcgg agctggatga ccgtgcagat
      241 gccctccagg caggggcctc ccagtttgaa acaagtgcag ccaagctcaa gcgcaaatac
      301 tggtggaaaa acctcaagat gatgatcatc ttgggagtga tctgcgccat catcctcatc
      361 atcatcatcg tttacttcag cactcgaatt ctgcagtcga cggtaccgta ggcccgggat
      421 ccaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
      481 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
      541 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
      601 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
      661 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
      721 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
      781 gagggcgaca ccctggtgaa ccgcatcgag ctgaagg
//
