LOCUS       sequence-130964-         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Zebrafish expression vector for heat-shock inducible expression of 
            APEX2-GBP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Ariotti N, Hall TE, Rae J, Ferguson C, McMahon KA, Martel N, Webb 
            RE, Webb RI, Teasdale RD, Parton RG
  TITLE     Modular Detection of GFP-Labeled Proteins for Rapid Screening by 
            Electron Microscopy in Cells and Organisms.
  JOURNAL   Dev Cell. 2015 Nov 23;35(4):513-25. doi: 
            10.1016/j.devcel.2015.10.016. Epub 2015 Nov 12.
  PUBMED    26585296
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2015.10.016"; journalName: "Dev Cell"; date: 
            "2015-11-23- 23"; volume: "35"; issue: "4"; pages: "513-25"
COMMENT     Sequence Label: HSP70l-APEX2-GBP in pDEST-Tol2-pA2-acrys-mCherry
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    132..156
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      157..166
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cttgtacaac taataataac ttggttatta agagaatttc acttgttgac tagaaaaatc
       61 ctttcataat gaaacaattg caccataaat tgtataaata taaaattaat tctaattgtt
      121 tttttttcct gccaagtttg tacaaaaaag caggctgcca ccatgggaaa gtcttaccca
      181 actgtgagtg ctgattacca ggacgccgtt gagaaggcga agaagaagct cagaggcttc
      241 atcgctgaga agagatgcgc tcctctaatg ctccgtttgg cattccactc tgctggaacc
      301 tttgacaagg gcacgaagac cggtggaccc ttcggaacca tcaagcaccc tgccgaactg
      361 gctcacagcg ctaacaacgg tcttgacatc gctgttaggc ttttggagcc actcaaggcg
      421 gagttcccta ttttgagcta cgccgatttc taccagttgg ctggcgttgt tgccgttgag
      481 gtcacgggtg gacctaaggt tccattccac cctggaagag aggacaagcc tgagccacca
      541 ccagagggtc gcttgcccga tcccactaag ggttctgacc atttgagaga tgtgtttggc
      601 aaagctatgg ggcttactga ccaagatatc gttgctctat ctgggggtca cactattgga
      661 gctgcacaca aggagcgttc tggatttgag ggtccctgga cctctaatcc tcttattttc
      721 gacaactcat acttcacgga gttgttgagt ggtgagaagg aaggtctcct tcagctacct
      781 tctgacaagg ctcttt
//
