LOCUS       sequence-130966-         737 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  express in mammalian cells for unnatural amino acid incorporation 
            into VAMP2-GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Vreja IC, Nikic I, Gottfert F, Bates M, Krohnert K, Outeiro TF, Hell
            SW, Lemke EA, Rizzoli SO
  TITLE     Super-resolution Microscopy of Clickable Amino Acids Reveals the 
            Effects of Fluorescent Protein Tagging on Protein Assemblies.
  JOURNAL   ACS Nano. 2015 Nov 24;9(11):11034-41. doi: 10.1021/acsnano.5b04434. 
            Epub 2015 Oct 28.
  PUBMED    26498474
REFERENCE   2  (bases 1 to 737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acsnano.5b04434"; journalName: "ACS Nano"; date: 
            "2015-11-24- 24"; volume: "9"; issue: "11"; pages: "11034-41"
COMMENT     Sequence Label: pEGFP-N1 VAMP2 R125TAG
FEATURES             Location/Qualifiers
     source          1..737
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      15..24
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(464..667)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(493..513)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ctcgcccttg ctcaccatgg tggcgaccgg tggatcccgg gcctacggta ccgtcgactg
       61 cagaattcga gtgctgaagt aaacgatgat gatgatgagg atgatggcgc agatcactcc
      121 caagatgatc atcatcttga ggtttttcca ccagtatttg cgcttgagct tggctgcact
      181 tgtttcaaac tgggaggccc ctgcctggag ggcatctgca cggtcatcca gctccgacaa
      241 cttctggtcc cgctccagga ccttgtccac attcaccctc atgatgtcca ccacctcatc
      301 cacctgggcc tgggtctgct gcagtctcct gttactagta agatttggag gaggtgcagg
      361 ggggccaccc tcgccggccg gggcggcagg cgggacggtg gcagcggtag ccgacataga
      421 tctgagtccg gtagcgctag cggatctgac ggttcactaa accagctctg cttatataga
      481 cctcccaccg tacacgccta ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt
      541 ttggaaagtc ccgttgattt tggtgccaaa acaaactccc attgacgtca atggggtgga
      601 gacttggaaa tccccgtgag tcaaaccgct atccacgccc attgatgtac tgccaaaacc
      661 gcatcaccat ggtaatagcg atgactaata cgtagatgta ctgccaagta ggaaagtccc
      721 ataaggtcat gtactgg
//
