LOCUS       sequence-13097-e        3714 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 3714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1439-HQ858741
FEATURES             Location/Qualifiers
     source          1..3714
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             112..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(1271..1370)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1387..1406)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1388..1406)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1411..1427)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1411..1427)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1529..1548)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1540..2349
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1607..1626)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2496..3084
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2985..3004
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3238..3255
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3414..3441)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3533..3619)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3668..3690
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3682..3699
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3683..3699
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc cttgtttaac
      121 tttaagaagg agcccttcac catggtggag cctagtgtga aatcttcaga gcatggtgga
      181 ggaggaatcg acctgctgtt cctgcctcct ggcttcaggt tccatcccac cgacgaggag
      241 gtcatcacca gctacctcct gcagaagctc ctcaacccta gcttcgcgcc gcacgccatc
      301 ggggaggtgg acctcaacaa gtgcgaacca tgggatctcc caagcaaggc gaagatgggg
      361 gaggaggatt ccaaggagtg gtacttcttc tgccgcaagg gcatgaaata cccgacgggc
      421 acgcgggcga accgcgccac caaggaggga tactggaagg ccacgggcaa ggacagggag
      481 atcttcttca agccagcagc tgggcacgac gacgtcgacc gccgtgagca gctggtgggg
      541 atgaagaaga cgctggtgtt ctacacgggc agggctccaa ggggcaccaa gaccaactgg
      601 gtgatgcacg agttccgcct catcgagggc aagggcaagt actccaggca ccgcaccacc
      661 aacgacgacc tactactacg cttcaacccc aaggatgaat gggtcgtgtg caaggtgcac
      721 cacaaagggg gccgacatga tgccagcgcc gccaagaagg gcggcggagg aggaggagga
      781 gaggagcagc aatactcgtc cgccgccgga acgcccaacg tcagctccgt cgaggccggc
      841 ggcggcggcg acgacgacga gttcctcctt gactccgtgc tgctggatta ctccagcagc
      901 tgccacttca actcctccgg tcccaccgct tgcacctcat ccagcaggag caggagcatg
      961 ccgccgcccc gtcgtcacgc gccgccggca gaccagcagc aagcaataat ggtgggaggg
     1021 agctactact acactagctt gcaccaccac caccaggaaa tggtcgcacc cacgaggttc
     1081 gccgccgccg ctggtttgcc gagtgcgtgt gcgtcggcgg cgggcgccgg agctgcgcag
     1141 cgtagttccc agcagcatgg tgtgctgcag cagcagaggc ttcccgccgg gaactacaac
     1201 tactacgacg acgggggaaa ctactacgct gcaggttacc gtacaagcca gtacaagggt
     1261 gggcgcgccg acccagcttt cttgtacaaa gttggcatta taagaaagca ttgcttatca
     1321 atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct
     1381 gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc
     1441 gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata
     1501 aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa
     1561 acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct
     1621 cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg
     1681 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg
     1741 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt
     1801 actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc attccaggta
     1861 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc
     1921 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc
     1981 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag
     2041 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca
     2101 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg
     2161 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt
     2221 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa
     2281 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag
     2341 tttttctaat cagaattggt taattggttg taacactggc agagcattac gctgacttga
     2401 cgggacggcg caagctcatg accaaaatcc cttaacgtga gttacgcgtc gttccactga
     2461 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     2521 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     2581 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     2641 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     2701 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     2761 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     2821 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     2881 cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     2941 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     3001 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     3061 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     3121 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     3181 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     3241 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     3301 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     3361 cgcaacgcaa ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc
     3421 catccgtcag gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg
     3481 cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc
     3541 tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact
     3601 gagcctttcg ttttatttga tgcctggcag ttccctactc tcgcgttaac gctagcatgg
     3661 atgttttccc agtcacgacg ttgtaaaacg acggccagtc ttaagctcgg gccc
//
