LOCUS       sequence-130995-         773 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses SpCas9 and Blasticidin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Arbab M, Srinivasan S, Hashimoto T, Geijsen N, Sherwood RI
  TITLE     Cloning-free CRISPR.
  JOURNAL   Stem Cell Reports. 2015 Oct 27. pii: S2213-6711(15)00284-2. doi: 
            10.1016/j.stemcr.2015.09.022.
  PUBMED    26527385
REFERENCE   2  (bases 1 to 773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2015 Oct 27. pii: S2213-6711(15)00284-2. doi: 
            10.1016/j.stemcr.2015.09.022."
COMMENT     Sequence Label: spCas9-BlastR (pCBhCas9-BlastR)
FEATURES             Location/Qualifiers
     source          1..773
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(3..50)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 tactttttct tttttgcctg gccggccttt ttcgtggccg ccggcctttt gtcgcctccc
       61 agctgagaca ggtcgatccg tgtctcgtac aggccggtga tgctctggtg gatcagggtg
      121 gcgtccagca cctctttggt gctggtgtac ctcttccggt cgatggtggt gtcaaagtac
      181 ttgaaggcgg caggggctcc cagattggtc agggtaaaca ggtggatgat attctcggcc
      241 tgctctctga tgggcttatc ccggtgcttg ttgtaggcgg acagcacttt gtccagatta
      301 gcgtcggcca ggatcactct cttggagaac tcgctgatct gctcgatgat ctcgtccagg
      361 tagtgcttgt gctgttccac aaacagctgt ttctgctcat tatcctcggg ggagcccttc
      421 agcttctcat agtggctggc caggtacagg aagttcacat atttggaggg cagggccagt
      481 tcgtttccct tctgcagttc gccggcagag gccagcattc tcttccggcc gttttccagc
      541 tcgaacaggg agtacttagg cagcttgatg atcaggtcct ttttcacttc tttgtagccc
      601 ttggcttcca gaaagtcgat gggattcttc tcgaagctgc ttctttccat gatggtgatc
      661 cccagcagct ctttcacact cttcagtttc ttggacttgc ccttttccac tttggccacc
      721 accagcacag aataggccac ggtggggctg tcgaagccgc cgtacttctt agg
//
