LOCUS       sequence-13100-e        4224 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 4224)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1442-HQ858744
FEATURES             Location/Qualifiers
     source          1..4224
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             112..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     RBS             874..885
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    complement(1781..1880)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1897..1916)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1898..1916)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1921..1937)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1921..1937)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2039..2058)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2050..2859
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2117..2136)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3006..3594
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3495..3514
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3748..3765
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3924..3951)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4043..4129)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4178..4200
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4192..4209
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4193..4209
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc cttgtttaac
      121 tttaagaagg agcccttcac catgaattcc agcagaagcc tcctctcttc gcccctcttt
      181 gctagctcgt ctccaaactt caggagcagt gcttccgtct cctcctcccc atccccatcc
      241 cgcaccacag ttccaatgat acatgacacc agcagcagca gagcatccac ggcgtgccac
      301 tactcgccgt cgcttgtggc agaggagcag cttcacggct ccaaagacac cttgaccttg
      361 aagggcgaga aggctttgct cgagttgctt ctaggcatgg ctctggacca gcacgttgac
      421 ggcaggaagc tcattaccca acaggaggct gaggatagtg atttcgagag ctatttgaga
      481 gaagcaacaa gccgagtgct ttaccagcca acattcattg aagaggatga tgatgatgat
      541 tctgtttcgg agagctcttc agcatcattg gccaaaccag ctggaacatt ggatcttggc
      601 aagctgacag aggaaccaca gttggatgta ccacagcctc accgccgcag tgtggatcca
      661 aaccattcgt atgatcaagt gtttattaga tccactcggt tactggagag gagatccaag
      721 aaacgcaacg gcggccacag ggctttggat aatggtgtgg cgtgcagtgg tgtgaactca
      781 aagagaaaag acaagtttgg cagagttctt gatccagacg agccttttag gttgtttcta
      841 cgggatcgag agacgacaga gttcttgaca gcgaaagagg agaaacagat gttcagtcaa
      901 atacagaatc ttatgaagct ggagggggct cagcgtaacc tgcaagctca gtgtggtcgt
      961 gaaccaacag ttgaagagtg ggctcaagcc gtaggaatga gctgcaggga gctgcaatcc
     1021 tgtgtgcgca ccggaagacg gtgccgagag aagatggccc gctccaactt ccgccttgtg
     1081 atacatgttg ctaggaaata tgaaggatgt ggccttgaca ttcaggacct ggttcaggat
     1141 ggatgctgtg ggttgatgaa aacctttgag aagttcaacc cgagcaaggg atgcagattc
     1201 ccgacctacg cgtactggtg gatacgccag tcgatcaaaa agtccatctt caagaattca
     1261 agactaatcc ggttgccgga gagtgtgtat gcacttttga ggaaggtggg caaagcgagg
     1321 atggaatgca taatggacgg ggagcagccg acgaacgagg tggtggcaag gcgtgctggc
     1381 atcacgattg aaaagctggc gaaactgaga gcgaaaacca ggaaagtacg gtccatgcag
     1441 gatcgagtct ggtcagacga cggtgtgaca taccaggaga tcaccgagga ccccaacatt
     1501 gaaggtcctg aggtgagcgt ggacaggctg atgatgcggc agcaggtgcg cagcttcctg
     1561 acggggatcc tgagcccccg ggagaaggag atcatcgagc accggttcgg catccacgac
     1621 ggccagccca agaccctgca tgtcatcggc gacatgtacg gcctgtccaa ggagaggatc
     1681 cgccaggtgc agaacaaggc cctggacaag ctcagaaaca gcatctcagc acagggcttc
     1741 cacgtctact ttgatttgct aactaagggt gggcgcgccg acccagcttt cttgtacaaa
     1801 gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt
     1861 caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc gtattacatg
     1921 gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca
     1981 caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca
     2041 aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg
     2101 gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca
     2161 atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt
     2221 agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg
     2281 cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact
     2341 gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat
     2401 attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt
     2461 ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt
     2521 ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg
     2581 aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca tggtgatttc
     2641 tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga
     2701 gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt
     2761 tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat
     2821 aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt taattggttg
     2881 taacactggc agagcattac gctgacttga cgggacggcg caagctcatg accaaaatcc
     2941 cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     3001 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     3061 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     3121 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     3181 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     3241 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     3301 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     3361 acgacctaca ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc
     3421 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     3481 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     3541 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     3601 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     3661 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     3721 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     3781 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     3841 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc
     3901 caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt
     3961 gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa
     4021 cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac
     4081 agataaaacg aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag
     4141 ttccctactc tcgcgttaac gctagcatgg atgttttccc agtcacgacg ttgtaaaacg
     4201 acggccagtc ttaagctcgg gccc
//
