LOCUS       sequence-131005-         761 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Samuels Y, Wang Z, Bardelli A, Silliman N, Ptak J, Szabo S, Yan H, 
            Gazdar A, Powell SM, Riggins GJ, Willson JK, Markowitz S, Kinzler 
            KW, Vogelstein B, Velculescu VE
  TITLE     High frequency of mutations of the PIK3CA gene in human cancers.
  JOURNAL   Science. 2004 Apr 23. 304(5670):554.
  PUBMED    15016963
REFERENCE   2  (bases 1 to 761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2004 Apr 23. 304(5670):554."
COMMENT     Sequence Label: PIK3CA-WT
FEATURES             Location/Qualifiers
     source          1..761
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      6..15
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             15..38
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cggccgccac catggattac aaggatgacg acgataaggc ccgggcggat cccccgggct
       61 gcaggaattc gatagttcga accatgcctc caagaccatc atcaggtgaa ctgtggggca
      121 tccacttgat gcccccaaga atcctagtag aatgtttact accaaatgga atgatagtga
      181 ctttagaatg cctccgcgag gctacattaa taaccataaa gcatgaacta tttaaagaag
      241 caagaaaata ccccctccat caacttcttc aagatgaatc ttcttacatt ttcgtaagtg
      301 ttactcaaga agcagaaagg gaagaatttt ttgatgaaac aagacgactt tgtgaccttc
      361 ggctttttca acccttttta aaagtaattg aaccagtagg caaccgtgaa gaaaagatcc
      421 tcgatcgaga aattggtttt gctatcggca tgccagtgtg tgaatttgat atggttaaag
      481 atccagaagt acaggacttc cgaagaaata ttctgaacgt ttgtaaagaa gctgtggatc
      541 ttagggacct caattcacct catagtagag caatgtatgt ctatcctcca aatgtagaat
      601 cttcaccaga atcgccaaag cacatataca ataaattaga taaagggcaa ataatagtgg
      661 tgatctgggt aatagtttct ccaaataatg acaagcagaa gtatactctg aaaatcaacc
      721 atgactgtgt accagaacaa gtaattgctg aagcaatcag g
//
