LOCUS       sequence-131007-         703 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses palindromic sgRNA for use in self-cloning CRISPR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Arbab M, Srinivasan S, Hashimoto T, Geijsen N, Sherwood RI
  TITLE     Cloning-free CRISPR.
  JOURNAL   Stem Cell Reports. 2015 Oct 27. pii: S2213-6711(15)00284-2. doi: 
            10.1016/j.stemcr.2015.09.022.
  PUBMED    26527385
REFERENCE   2  (bases 1 to 703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2015 Oct 27. pii: S2213-6711(15)00284-2. doi: 
            10.1016/j.stemcr.2015.09.022."
COMMENT     Sequence Label: sgPal7 (p2Tol-U6-sgPal7-HygR)
FEATURES             Location/Qualifiers
     source          1..703
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     109..128
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     polyA_signal    410..531
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     475..494
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 attggaatta atttgactgt aaacacaaag atattagtac aaaatacgtg acgtagaaag
       61 taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatcatatg
      121 cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga
      181 aacaccggct tagtactagt actaagcgtt taagagctat gctggaaaca gcatagcaag
      241 tttaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttg
      301 ttttagagct agaaatagca agttaaaata aggctagtcc gtttttagcg cgtgcgccaa
      361 ttctgcagac aaatggctct agaggtacgg ccgcttcgag cagacatgat aagatacatt
      421 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
      481 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
      541 aattgcattc attttatgtt tcaggttcag ggggagatgt gggaggtttt ttaaagcaag
      601 taaaacctct acaaatgtgg taaaatcgcg aaggtcgacg gtatacagac atgataagat
      661 acattgatga gtttggacaa accacaacta gaatgcagtg aaa
//
