LOCUS       sequence-131010-         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Base plasmid for sgRNA cloning with Hygromycin resistance and Tol2 
            transposon arms.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Arbab M, Srinivasan S, Hashimoto T, Geijsen N, Sherwood RI
  TITLE     Cloning-free CRISPR.
  JOURNAL   Stem Cell Reports. 2015 Oct 27. pii: S2213-6711(15)00284-2. doi: 
            10.1016/j.stemcr.2015.09.022.
  PUBMED    26527385
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2015 Oct 27. pii: S2213-6711(15)00284-2. doi: 
            10.1016/j.stemcr.2015.09.022."
COMMENT     Sequence Label: sgBbsI (p2Tol-U6-2xBbsI-sgRNA-HygR)
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     109..128
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     polyA_signal    407..528
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    652..773
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 attggaatta atttgactgt aaacacaaag atattagtac aaaatacgtg acgtagaaag
       61 taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatcatatg
      121 cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga
      181 aacaccgggt cttcgagaag acctgtttaa gagctatgct ggaaacagca tagcaagttt
      241 aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc ttttttgttt
      301 tagagctaga aatagcaagt taaaataagg ctagtccgtt tttagcgcgt gcgccaattc
      361 tgcagacaaa tggctctaga ggtacggccg cttcgagcag acatgataag atacattgat
      421 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
      481 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
      541 tgcattcatt ttatgtttca ggttcagggg gagatgtggg aggtttttta aagcaagtaa
      601 aacctctaca aatgtggtaa aatcgcgaag gtcgacggta tacagacatg ataagataca
      661 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
      721 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttggggtgg
      781 gcgaagaact ccagcatgag atcc
//
