LOCUS       sequence-131022-        1692 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For bacterial expression of Dnmt3a-Dnmt3L single-chain fusion 
            protein protein. Contains the catalytic domain of mouse Dnmt3a and 
            the C-terminal part of mouse Dnmt3L connected by 27 aa linker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Siddique AN, Nunna S, Rajavelu A, Zhang Y, Jurkowska RZ, Reinhardt 
            R, Rots MG, Ragozin S, Jurkowski TP, Jeltsch A
  TITLE     Targeted methylation and gene silencing of VEGF-A in human cells by 
            using a designed Dnmt3a-Dnmt3L single-chain fusion protein with 
            increased DNA methylation activity.
  JOURNAL   J Mol Biol. 2013 Feb 8;425(3):479-91. doi: 
            10.1016/j.jmb.2012.11.038. Epub 2012 Dec 4.
  PUBMED    23220192
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jmb.2012.11.038"; journalName: "J Mol Biol"; date: 
            "2013-02-8- 8"; volume: "425"; issue: "3"; pages: "479-91"
COMMENT     Sequence Label: pET28-Dnmt3a3L-sc27
FEATURES             Location/Qualifiers
     source          1..1692
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..57
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat
       61 atgaaccatg accaggaatt tgacccccca aaggtttacc cacctgtgcc agctgagaag
      121 aggaagccca tccgcgtgct gtctctcttt gatgggattg ctacagggct cctggtgctg
      181 aaggacctgg gcatccaagt ggaccgctac attgcctccg aggtgtgtga ggactccatc
      241 acggtgggca tggtgcggca ccagggaaag atcatgtacg tcggggacgt ccgcagcgtc
      301 acacagaagc atatccagga gtggggccca ttcgacctgg tgattggagg cagtccctgc
      361 aatgacctct ccattgtcaa ccctgcccgc aagggacttt atgagggtac tggccgcctc
      421 ttctttgagt tctaccgcct cctgcatgat gcgcggccca aggagggaga tgatcgcccc
      481 ttcttctggc tctttgagaa tgtggtggcc atgggcgtta gtgacaagag ggacatctcg
      541 cgatttcttg agtctaaccc cgtgatgatt gacgccaaag aagtgtctgc tgcacacagg
      601 gcccgttact tctggggtaa ccttcctggc atgaacaggc ctttggcatc cactgtgaat
      661 gataagctgg agctgcaaga gtgtctggag cacggcagaa tagccaagtt cagcaaagtg
      721 aggaccatta ccaccaggtc aaactctata aagcagggca aagaccagca tttccccgtc
      781 ttcatgaacg agaaggagga catcctgtgg tgcactgaaa tggaaagggt gtttggcttc
      841 cccgtccact acacagacgt ctccaacatg agccgcttgg cgaggcagag actgctgggc
      901 cgatcgtgga gcgtgccggt catccgccac ctcttcgctc cgctgaagga atattttgct
      961 tgtgtgtcta gcggcaatag taacgctaac agccgcgggc cgagcttcag cagcggcctg
     1021 gtgccgttaa gcttgcgcgg cagccatatg ggccctatgg agatatacaa gacagtgtct
     1081 gcatggaaga gacagccagt gcgggtactg agccttttta gaaatattga taaagtacta
     1141 aagagtttgg gctttttaga aagcggttct ggttctgggg gaggaacgct gaagtacgtg
     1201 gaagatgtca caaatgtcgt gaggagagac gtggagaaat ggggcccctt tgacctggtg
     1261 tacggctcga cgcagcccct aggcagctcc tgtgatcgct gtcccggctg gtacatgttc
     1321 cagttccacc ggatcctgca gtatgcgctg cctcgccagg agagtcagcg gcccttcttc
     1381 tggatattca tggacaatct gctgctgact gaggatgacc aagagacaac tacccgcttc
     1441 cttcagacag aggctgtgac cctccaggat gtccgtggca gggactacca gaatgctatg
     1501 cgggtgtgga gcaacattcc agggctgaag agcaagcatg cgcccctgac cccaaaggaa
     1561 gaagagtatc tgcaagccca agtcagaagc aggagcaagc tggacgcccc gaaagttgac
     1621 ctcctggtga agaactgcct tctcccgctg agagagtact tcaagtattt ttctcaaaac
     1681 tcacttcctc tt
//
