LOCUS       sequence-13105-e        3114 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1455-HQ858749
FEATURES             Location/Qualifiers
     source          1..3114
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             112..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(671..770)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(787..806)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(788..806)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(811..827)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(811..827)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(929..948)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             940..1749
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1007..1026)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1896..2484
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2385..2404
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2638..2655
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2814..2841)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2933..3019)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3068..3090
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3082..3099
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3083..3099
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc cttgtttaac
      121 tttaagaagg agcccttcac catggaagca gtgatgggtt gctgggagct tgaacaatct
      181 ttacaaagcc taacaggggc atctcctggt gaaggcactg gagcgaccat gtctgatgat
      241 gaagacaacc aggtggatag cgaagccaac atgtttgatg gaaatgatgg atcagatggt
      301 atgggctttg gccccttaat gctgacagag ggtgaacgat ccttagttga gcgtgtacgg
      361 caggagctaa agaacgagct taagcagggt tacaaagaaa agcttgttga tatcagggaa
      421 gagattatgc gcaagcgcag agctgggaaa cttcctggag atactgcatc tgtgctgaaa
      481 gcttggtggc aagctcattc caagtggcca tacccaactg aagacgacaa ggcacgactg
      541 gtgcaggaaa cgggattgca gctaaagcag attaacaact ggttcatcaa ccaacgcaaa
      601 cggaactggc acagcaaccc aacatcctcg ggtgaaaaga ctaagaagaa aaggaagggt
      661 gggcgcgccg acccagcttt cttgtacaaa gttggcatta taagaaagca ttgcttatca
      721 atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct
      781 gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc
      841 gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata
      901 aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa
      961 acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct
     1021 cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg
     1081 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg
     1141 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt
     1201 actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc attccaggta
     1261 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc
     1321 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc
     1381 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag
     1441 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca
     1501 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg
     1561 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt
     1621 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa
     1681 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag
     1741 tttttctaat cagaattggt taattggttg taacactggc agagcattac gctgacttga
     1801 cgggacggcg caagctcatg accaaaatcc cttaacgtga gttacgcgtc gttccactga
     1861 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     1921 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     1981 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     2041 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     2101 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     2161 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     2221 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     2281 cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     2341 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     2401 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     2461 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     2521 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     2581 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     2641 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     2701 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     2761 cgcaacgcaa ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc
     2821 catccgtcag gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg
     2881 cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc
     2941 tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact
     3001 gagcctttcg ttttatttga tgcctggcag ttccctactc tcgcgttaac gctagcatgg
     3061 atgttttccc agtcacgacg ttgtaaaacg acggccagtc ttaagctcgg gccc
//
