LOCUS       sequence-131053-       14764 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloned region contains Acetobacter aceti 1023 rpmE-radC(and beyond) 
            region.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14764)
  AUTHORS   Constantine CZ, Starks CM, Mill CP, Ransome AE, Karpowicz SJ, 
            Francois JA, Goodman RA, Kappock TJ
  TITLE     Biochemical and structural studies of N5-carboxyaminoimidazole 
            ribonucleotide mutase from the acidophilic bacterium Acetobacter 
            aceti.
  JOURNAL   Biochemistry. 2006 Jul 11;45(27):8193-208.
  PUBMED    16819818
REFERENCE   2  (bases 1 to 14764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2006-07-11"; volume: "45(27)"; pages: 
            "8193-208"
COMMENT     Sequence Label: pJK193
FEATURES             Location/Qualifiers
     source          1..14764
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(66..83)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(237..825)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(317..336)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(996..1787,1788..1856))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCHTLLSRIDAGQEQLGRRARYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1857..1961)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1999..2018
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1999..2017
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             10889..10897
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     promoter        complement(14695..14713)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    14720..14753
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
       61 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
      121 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      181 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
      241 gataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
      301 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      361 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
      421 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
      481 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
      541 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
      601 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
      661 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
      721 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
      781 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
      841 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
      901 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
      961 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     1021 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     1081 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     1141 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     1201 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     1261 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttggcattgc tacaggcatc
     1321 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     1381 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     1441 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     1501 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     1561 tcattctgag aataccgcgc ccggcgaccg agttgctctt gcccggcgtc aatacgggat
     1621 aatagtgtat gacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     1681 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     1741 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     1801 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     1861 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     1921 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     1981 ccacctgacg tcgcggccta atacgactca ctatagggcg gccgcggatc cttcgtgcgc
     2041 accgtaccgt tggtgataac ctttttgata ccggcatcaa tacggtcagc cagatcattc
     2101 cggtttacat ggcgcagcat cataacggct gccagcagca gagccagcgg gttggcaatg
     2161 cctttgcctg cgatatcagg cgcagaaccg tgcacggctt caaacacagc ggctttttca
     2221 ccaatgtttg cgcccggtgc catacccaag ccaccaacca gaccggccgt aaggtcagaa
     2281 aggatgtcac caaacaggtt ggtggtcaca atcacatcaa actgccatgg gttgataaca
     2341 agctgcatgg cgcaggcatc tacaatccgt tcattgaatt caatgcggcc cttgtattct
     2401 tctgcaacct tgcggccttc ttcaaggaac aggcctgtca gcagtttgag gatattggcc
     2461 ttgtgaacaa tggtcaccgt tttacggttg ttcttgaccg catattcaaa ggcgaatttt
     2521 acaatccggc ggcattctgc gcgggagtta aagccggagc tcagcgcaat gcctttgggg
     2581 tcttcaccca ccggaatgta gtgttccatt gctgcgtaat aaccttcaag gttttcacgg
     2641 aacagaacaa ggtcgatatc atcaaaccga ccaccgggaa tgatggtttt ggttgggcgc
     2701 aggttggcaa acaggccaaa ttcctgacgc agggttacgt tgatggattt gaacccacca
     2761 ccaacaggcg ttgtcagcgg gcctttcagg gccaggcccg tgcgccggat gctttcaatt
     2821 gtctgtttgg gcagcgggtc atttaccgcg tccacacccg ccataccggc cagctggcga
     2881 tcccatacaa atggggcacc aacagtatcc agaatttcgg taactgattc aacaatttca
     2941 gggccgatgc catcacctgc aataagagtg gctggaatgg tcttgctgtc tgtcgtcatt
     3001 ctgagaagct cccttcaact tcgtagcctg ttttgcgccc gttttacggt gcacgcgatt
     3061 ctcaatttcg tgaccagagg ggaaagactc cgtctctcac gccctcttga gaggtgcgca
     3121 tgccgttttc agccatttct gtgcatcaga tggcaggtga gggcctacct gatccaagat
     3181 ttgagcatgg tacacatcca gcacggccgt atcttccggg cctaacaggg ccgggtcaat
     3241 cagccgcctg tcaaaagggg ccaatgtaag ggtttcaaat tccagaaaag cacgctcaga
     3301 atctgtcgta tgatcggggc ggaccatcac cagtgtttcc aaccggatgc cgtaagcgcc
     3361 gggtttgtaa aatccgggct cgttggaaat taccatgcct tcctccaacg caatgggaga
     3421 gggcattttg gaaatacggg cgggcccttc atgcacggaa agaaagctgc caacgccgtg
     3481 gccagtgcca tgatcataat ccagcccggc ctgccacagg tcaaaccgtg ccagtgcatc
     3541 cagtgcatgg cctttggtgc caaccggaaa acgtgcacgg cccaagcgca gattgccttt
     3601 cagcacgcgg gtaaagacgt ctttcaagct agcaggaggg gcattggggc ccgtccagac
     3661 tgtgcgggtc acatctgttg tgccttcagg gtactggccg ccgctatcaa tcaaatagac
     3721 ttcgtcgtcg tgtaattttc gatcactttg cggcgtgaca cgataatgaa tgattgcgcc
     3781 attggggcca gagccggaaa tggcgggaaa agattcttcc cggtagtcct tgccttcagc
     3841 ccggaaggca tcaagccgtt tggcggcttc aagttctgtg cagttgtggc cttttgtatc
     3901 cagccaatgc aaaaaacggc acagggccac gccatctcgc agatgggctg tgcgcatgcc
     3961 agcctgttcc accttgtttt tgcacgcttt gggcagcagg cacggatctg gtgtttcctg
     4021 cacggttgca ccataccggg taagggtttg gccaaaccag acagcattgc tggcaggatc
     4081 aaccccaacg gttttaccgt tgagcgttgc tagaacctgc tccatatcct gcggcgcgtg
     4141 aacacgcaca gaagtgccga gccagtcttt gatatttgtc gaaacttttt gtggtgcaat
     4201 aaacagatca accgagctgt ttgcgtgcac cagtgcaaat gccagcacca caggtgtgca
     4261 ggggatatcg gaaccacgaa tattcagtag ccatgcaata gaggctgaat cacttaaaac
     4321 cgcagcatcc tggccattct gcgtaagaat tgcagaaatc tcctgccgct tttgaacaga
     4381 gcttttcccc gcaaaggcaa ggggatgtac acaggctggt gatgcgggaa atgcagggcg
     4441 gtctgcccag atatcatcta ccagattgcg cgtggtgggt accagtttca ccccactttg
     4501 ggctgcgaac gggcgcagtt ccgcaacact cataacgcgc ggatcatacc ccacgcgggt
     4561 ttccggtttg gcttgctctt tcagccataa ggcgggcggt gtctggctaa tatgcaggcg
     4621 ttgccagcaa gtgccatcta cctgctgatc catttgagta atgtagcgtc catcagaaaa
     4681 cacggcggct gtgtgcggta gtacaatggc ggtgcctgcg ctgcctgtaa aacctgttag
     4741 ccatgccaga cgctctgcgc aggctggtgt gtattccccc aaaaattcat cgctatgggg
     4801 cacaatcagg ccatccagct cttcgcgttg caggagggtg cgcagtgcgg caagacgggt
     4861 agaagatgca gacgccatga aaaccgtgtc ctgaaataac gtaggaaaag ataaagctgc
     4921 ttaggctgtt ttgcggatag gaagattggg gcggcgcagg ataagggtgg cccattctcc
     4981 ctccaccaat tcctgctcca acaccaaacc ctgccggcgg tgtgctgcca gcaccatgcg
     5041 cacctgtgtg cgcagcagcc cggccagaat aactgtgcca ccgggggcca gataccgcgc
     5101 cagatccttg gccatcaggc ataaggggcg tgccagaata ttggcaaaaa ccagatcaaa
     5161 cggggcggct ttccaaatgg caggggtttt ccagccattg cccagccggc atgtcatggc
     5221 ccgccccagc tggttacgct tggcattttg tgccgctaca cgcacagacc acggttcaat
     5281 atccacagcc aggacgttct ggtgcaataa tgccgcagca gccatggcca gaataccggt
     5341 gccacaaccc agatccagaa tacggcgcgg gtggcgataa gccacacgtt ccagcgcacg
     5401 caggcacccg cgggtagaac catgctcgcc agatccaaaa gccacgcccg catccagtgt
     5461 cagcacaatg cgtgtggcat ttttggaatc gggcaaatgc gtgccacgca ccacaaagcg
     5521 gcggcccact tcctgttcgg gaaaagcctc atacgtgcgg gccagccagc cttcggcttc
     5581 tgtgctggtg cgttccagcg gggcactgac accactggtg agttctgcca acgcaagggc
     5641 tgctgccagt tcatcttccc ggtggccggt gtcttttaca ccttccaccc gccattgggt
     5701 gccttcgggg ttggcttcaa aaatgccaac tgtggcgcat acgctgccaa tggcgttttc
     5761 gtaagcttct accgccgctt ccggcacagt gacagaaatg gtttcaaggc tggtggcatg
     5821 acggcgagaa ggaggggcca ttagattttc tcgataaagg atgaaagaag acgtttggtg
     5881 ccaacagaat caaaagacac ttcaacatgg tgtcgatctg ctgaaatcac gataccttcg
     5941 ccaaagcggg ggtggcgcac atggtcgccc agttgcaggg ctggtgcggc ctccagcatg
     6001 gggtcttttc tacggcgggc aataaggggg ccgttatcaa atacggattc ggacaggtta
     6061 agcccctgcc gctggaatgt gccggcttca tcatggcgca gtacgtgctc atctggcagt
     6121 tcatccagaa agcggctggg catggagggc tgccagttgg cgtaaatacg ccggcggcca
     6181 gcatgaaaga taatggcctc ctgccgggca cgtgtaatgc ccacataagc caacctgcgc
     6241 tcttcctcca accctttcag gccgccttca tccagcgtgc gctgggaggg aaaaacgcct
     6301 tcctcccatc cgggcagaaa aaccgtatca aactccagcc ccttggcgcc gtgtagcgtc
     6361 atgatggaca ggtaatcatc ctgcccgttt tcatcatgat ccataacaag agcaacgtgc
     6421 tccagaaact cgcccagagt tccaaaatcg gccagtgcgc gcgtcagttc tttcaggttt
     6481 tccaaccgcc ccggtgcatc gggggagcga tcctgccgcc acatgtccag atacccgctt
     6541 tcttccagca ggtgttctac ggccaccaca tggccttcac gcgccagaat atcgcgcgtg
     6601 ctggtaaagg cttccatcaa ctggcgtaaa tgggcggcag attttccttt aagggcgtta
     6661 tcttccagca gtttttccgt gccgcctgta aggggcaggt tattggcgcg tgctgctgca
     6721 tgtagctttt gcagcccggc cgggccaaca ccacgtttgg gcacgttgat aatgcgttca
     6781 aaagccagat catcggccgg ttggctcagc acgcgcatat aggccagtgc atcacgtatt
     6841 tctgcgcgtt cataaaaacg caggccgccc acaatgcggt aaggcaggcc aatttgcatc
     6901 aggcgttctt caaacgcgcg ggtttgaaag cccgcacgta ccagaatggc catttcgttc
     6961 agggcatgac cttgcccgcg cagtttctcg gcgtgttcgc acaccatgcg ggcttctgta
     7021 tccgaatccc aaacaccagt gacgctaacc tttttacctt ctgcatcttc tcgaccgggg
     7081 cggagtgttt tgcccagccg ttcgctatta tgggcaataa gccctgcggc agcccccaga
     7141 atatggcggg tagagcgata attgcgttcc agccgtacaa ccttggcgga ggggaaatct
     7201 ttttcaaacc gcagaatgtt ttcaacttcc gcgccgcgcc atgagtagat ggactgatca
     7261 tcatccccca cacagcagat attggccggg ccttcggcat gtttggccag caggcgcagc
     7321 cagagatact gaatggtgtt ggtatcctga tactcgtcca ccagaatgta acggaacagg
     7381 cggtgatact gggctagcac atctggccgt gtgcgcagga tctcggtcac atgcagcatc
     7441 aggtcaccaa aatcgcaggc gtttagctgc ttcaggcggg cctgatacgc ggtgtaaatg
     7501 gcgcgggcat ggccattggc aaagtccgaa tcctcggtgg gggtaatgcg ctcgggggtt
     7561 agcccacggt ctttccagcg ttggatcaca cctaaaaggc ccggtgctgg ccaacgtttg
     7621 atatcaatct gccacggtgc catcacctgt ttaagcaggc gtagctggtc atccgtatcc
     7681 agaatggtaa agctttgtgt cagccccaca tattccgcat ggcggcggag catacgcgcg
     7741 catagggcat gaaatgtgcc caaccataac ccttctgccg ggctacccaa cagcaggctt
     7801 atacgctcac gcatttcgcg cgcagctttg ttggtaaagg ttactgtcag gatctgccac
     7861 ggcttggcac ggcccgtaag caggatatgt gcaaagcggg tggttaaaac ccgtgttttg
     7921 cccgtgccag caccagccag cacaagcaga gggccatccg ttgtttcaat agctgcgcgc
     7981 tgttcggggt tgagacgagc cagataactg tcacccgagg cagacggaga tgaaaaaagc
     8041 ggggcggagg ggtgttcgga cgtattcacc atgcctttat ataagcatgt gcgcttcagg
     8101 ccaaacggag caatggaatg gagagacagt cagcgggaca gaaaaggcaa aaaaagcctg
     8161 agcattccgt tgcaaaggct ggcacaaccc aaggttgtca atctgcgcct caagcttcca
     8221 tacctgcttt tgaaagtact ggccccacag ggcaccgtaa acgcatgcgc cagcgggtaa
     8281 tggcgcaggg agccgctgct cttgctgatt atgaattgct ggaaatgctg ctgtatgttg
     8341 gggttccgcg taaggataca aagccgctgg ctaaaagcct tatcaatgcg tttggctctt
     8401 ttcaggcagt tatagaagca aaagaagaag atctgcgcca aattggcggt gtaacgcgcg
     8461 ccagtgcggc actgttgcag aatgtggccc gcgtggcaga caggttgcca gaaaatggtg
     8521 cagaatcacg ccgttttttg gggaactggg acagtatttt agaatatgca gatgtgtatc
     8581 tggcgcatac gcccaaaggg caggtgcggg ctttgtttct ggatagccgt aaccagttga
     8641 ttgcggatga aactgtgccg gcctttttgc atgaagacag cagccatgaa gccatggcct
     8701 gtattcggca tgctgtggcg caggttttgc aacgtgcact ggccttgcat gcctgcgcat
     8761 tggtaactgt ggatctggta gcggaagatc aaacgctgga tgaaagcctt acacaaaatg
     8821 caccgtttgt gcgggaatta cagcgtagcg ccccattgtt ggctgtggag gtgcatgatc
     8881 atattgctat aggacgcgga gaatggcgca gctttaatca ctctgcaggt tcaaaatctt
     8941 gaagcgtccg cccacaaagt cagaaaaaaa tgccaccggt caatttgctg atgccacaca
     9001 tttccggctg aatgttgcaa ctgcgcaaac cgttccattt gccgccgaaa tgcatgagcg
     9061 cgcagagcag acgtttccgg gagcagaaga tacagatttt accctattat gcagtcttat
     9121 attgcaagcg cagccccgga atataaaagc aaatgaacag gcacagaatt tattgcaaga
     9181 atatggaagt ctggcagcag taatggctgc cgtaagttgg caggaaacaa gtaaaagtat
     9241 aaaaactttg ccagaagctg tgcgtgttct gcttatgctg gtgcgtgaat ccgggttgcg
     9301 tatccagcgt gcaagattgc aacattccgg catattggca gaacaacaac agctgtatgc
     9361 ttatttgcgg gccataatgg ggccggaaaa acgcgagcag gtgcgtgttc tttttctaaa
     9421 tgaccagcat caacttctgg ctgacgatat tatggggcag ggtaccgtag accatactcc
     9481 cgtttacccg cgggaaattg tagcccgcgc gctacaatta aaggcaaccc tgttggtatt
     9541 agtgcataac caccccagcg gtgacccaac accttcagcc gatgatgtgg tgatgacaaa
     9601 ccaaatctgt ggggcggcaa aaattatgaa tatccatgtc tgggatcata tcattgttgc
     9661 tggagaaaag ctgttgagca tgcgtgaagc agggtttttg taagtatctg cttcacgcag
     9721 tgccttaact ggcagattct tcatcttcca cagcggccgt tttggtggcc aatgggccca
     9781 aggcatcctg aatacccaaa ttgcctggca gcgcaccgta ggggaacaga atatttacat
     9841 gccccatttt acggcccact ctggcttctg ccttgccata tagatgcgga ataaagccct
     9901 gcgtggccat aatctgcggc agcagcgcca tatcttccgg cccgaccaga tttttcatga
     9961 ttgcatcaga atggcgcaca gcgtgcggca aaggcagccc cgcaactgca cggatatgca
    10021 tgtcaaattg gtcctgcggg caggcattca tggtccaatg gccagaatta tgggggcgcg
    10081 gggccatttc attcaccagc aggttgccgg tgcaatcaac aaacatttca acgcccagaa
    10141 taccaaccag ctccagcttt tctgcaatgg tggtggctag ttttatggcc tgcgtggcaa
    10201 tatctgggtc aatgggggcg ggggctaatg ttagatccag aataccgttc cagtgccggt
    10261 tcagcgccgg gtcaaagcac cttaccgtgc catctgcacc acgcgccacc ataacactaa
    10321 tctcgcaagc aaaatccacc ataccttctg caaccagagg atgtggtgcc agagcgttga
    10381 aagcctcttc cagatcatgg atgtgatgca cacggcgctg gcctttgcca tcatatccat
    10441 tgcgagtggt tttgagaatc agcggcaaac ccactttttc tgcggcttcg tgtagcgttt
    10501 tgacatcggt aacaggatgc catggcgcga gtggaatacc cgcagaagca aaaaaagttt
    10561 tttctgccaa tcggtcctga ctgatgcgca gaatatgccc cgaaggatga acagggcagt
    10621 gtttggctag gcggtccagt gcatctgcgc tgatgttttc aaattcaaag gtaacaacat
    10681 caacagcgcg ggcaaaggca tccagtgctg tcggatcatc atacttgccg catgttacgg
    10741 cgtgagagac ctgagccgcc gggccgttgg cctctggcgt aagaatatgc gtgcgaaaac
    10801 caaggcgcgc ggcagcaaca gcagacatgc ggccaagctg gccacccccc acaatgccaa
    10861 taacggcatt gggcttaagc ggagaatgta aggaggtcat ttatcttcgg taatgggaga
    10921 gttggggaca gaggccgtct gcaatgcgcg ccatgtttca aggcgtgcgg caagggccgg
    10981 gttatacagt gcaagaatgg aggcggccag caaggcggca tttttggcgc cggatgcccc
    11041 aatagccaaa gtgcccacag gcacgccgcc gggcatctgc acaatggaaa gcaggctgtc
    11101 catgccttta agagcgcgag attctactgg aacacccagc acgggcaggc gtgtccatgc
    11161 cgcacacata cccggcagat gtgcagctcc accggcccca gcaataatca cattcaggcc
    11221 gcgttcggct gccgtacgcg cataatccgc caaccggtct ggcgtacgat gtgctgaaac
    11281 aatgagggtt tcatgcggaa tttccagctc tgtcagcaat gcgtctgcat ggcgcatggt
    11341 ttcccagtct gactggctgc ccatgataat gcccacaacg ggggcagagg ctgctttatc
    11401 ttctaaagca ctggaagcag agggcagggg ggcggtttcg ctcacggtgg gtatctatcc
    11461 ggttttttaa agtctgtaac ggagaggttc aggcaatgtc atcagggatc agaaggcttt
    11521 caaccttggt aatctgatct tttagcagaa gtttacgttt tttcagccgc tggagataga
    11581 gttggtcaat aaaagtagtt tcatgcgcca tgcggttgat aaccgtatcc agatctctgt
    11641 gttcactccg gagttcatgc agttgggcaa gcagtaaatc gcgatctcgc agcatgatgt
    11701 cctgtctcct tcaccattgg tgcggaatcg tgcgccgggt gtggcggccc gcaaagtgaa
    11761 tagcatgccc gaagcagtgt ttaccatgta aggcccggac gccagccagg attacaaaaa
    11821 aacttccatg ctcaggctgc ataccagaag ggcgcgatat atgaaattaa catgaacttt
    11881 cttgacctcg gtacgttagc gttgacgggt catcaatcga gtggaggcca caaatgacac
    11941 gcctttcaag gatcgaaagt cttaaaacgc gccatttccg tattgatcag aaaattatga
    12001 gcgaaggcgg acgccccaga ccggacgcgc gcgtgttgat gtgcctgaaa cttcagaaat
    12061 tgcgaattaa ggaagaaata gagcgtcttt caacctgata ttcaatttgg cacactgaac
    12121 atttctgagg cagaaaacga ttgtttttct gcctcagact ataacgcggt ttattcttca
    12181 gaaatactaa agctttccac gtctgtaaca ggtgcaggaa tttcctgccc ttcacgttcc
    12241 agacgcatat tggcttcatg cacggcagcg ttcaggtctg tctggctgca cagccccacc
    12301 gttaccggat cacgcggctt gatgttgggg gtgttccagt gctgcttgtt gcgcactttt
    12361 gcaatggtat ctttggtggt gccaagaagc ttgacaatct gctgatcgct cagctgcgga
    12421 tagttgcgta gcagaaaagc aatgccatct ggacgatcat gacgcttggc aacaggcgta
    12481 taacgcgccc ctttggagcg gcggcgtaca ggattgttgg ttggcagaat tttcagccgt
    12541 gcgttggggt ctttctcaca acgctggatt tcttcctgct tgagctgatg attcgcaatc
    12601 ggatcataac cgacaatgcc atgtgccact tcgccatctg caatggcctg cacttccaac
    12661 gggtgcatac cgcaaaaagc ggcaatctgt tcaaaactga gcgctgtttt ttcaatcagc
    12721 cacacagcgg ttgctttggg cattaacggt agggtcgtca tggttttgta ttcctgtcgt
    12781 cgggcggcgc ttgcgcaggc accgggaact tgacggcccg gaacgtgttc cgcaggcctg
    12841 cgtgcctgcc ggcgcgcatc tgtgccacga tatgggggga gtggctgctt caggtcaagc
    12901 cgcaatgcac acaacaccta cattctgcac aaataaaata aggggaagcc gcagcttccc
    12961 cttaccttgc caatatatgg tttatgcgca ctgtgccgtt atcaggccgc tgtagctatg
    13021 ctttgtgggg cctgcggttg tgtcggctgt tgagtggcgg aaaccacggt atgttccgca
    13081 ggtgtggaat tgacggtttt aaccggaatg cggcgtggtt tgagtgcatc gggaataatt
    13141 tgttgcacag taatacggag caagccgttg ttcatttccg cgccggtcac ttcaacatga
    13201 tctgccagtg caaaacggcg ctcaaacgcg cggcgtgcaa taccacgatg caagatttcg
    13261 ccttcatcct gcggtgtagt gacacggcca gaaacaacaa gcgtgttatc ctgcacaatc
    13321 aattcaatat catccggccc aaagccagct atcgccattg tcagcgtgta ccgatcattt
    13381 cccgttttag cgatatcata aggagggaag ctggaattgc cgggagaccg ggcagcgcta
    13441 tccatcacct gtgccaggcg gtcaaaccca attgcagtac gcagcagcgg tgtaaaatca
    13501 taactcatgt aagaacctcc tcgttcagca aggttctgcc ggttttgcca agacccccga
    13561 aggcaatctg cccggcagcg ttgtttggcg gaaaccccat atacgggcat ttccatacct
    13621 ctgaacgtgg aaagccccgc actctttttc aagaggcggg gcttttgaac ctgcgtaaag
    13681 aactgaaaaa tcagcctttc ttgcgggtgc caataccagc aaaacgcttg ttgaacttgg
    13741 caacctggcc cccggtatcc agcatacgct gcacgcctgt ccatgctggg tgagacttgg
    13801 ggtcaacatc caggcggagg gtgtcgccag ccttgccata gcaggaatgc gtcttgaatt
    13861 ccgtgccatc ggtcatgaca acattgattt cgtgataatc ggggtgaatg ccgggtttca
    13921 tgaaactggt tctttcgtta cagcagaaag ggcccgatac cgaccgggcc aagagtgccg
    13981 tgcatataaa cgaaatgcgt gtgttggatc aaccgttcat tttgttttgc ttggcaagat
    14041 gaagatgcgc cgcaaataaa tggccaatta caccgtgttg tgctgcattt gtgctacaga
    14101 acgcatgaaa tattaagaaa agtctatcca tttggatact atgggcttga gagcgcagcg
    14161 ctaaaacggc taattcaggg cagtatttcc cagtatttcc cagtatttaa caggattttt
    14221 tgtatggctg accttaagtc tcacgtccat gccccaggtt gggtgggcga attccgggca
    14281 ttcattatgc gcggtaatgt tgttgatctg gctgttggtg ttattattgg tgctgcgttt
    14341 acatcaattg taaacagctt ggtaaaagac attttcaacc cacttattgg ccttcttatt
    14401 ggcgggattg atttttccaa cctgtttatc acccttaaag gcccgcatct ggccactctg
    14461 gcagaagccc agaaagccgg tgccgttacg ctgaacattg gcctgttcct gaacgctgta
    14521 atccagtttc tgattgtggc catggccatt ttctggatgg taaaagctct gacacgcctg
    14581 catgtgcgtg aagatgaaca gcccaaggca ccgccgccgc cctccaaaac cgaagttctg
    14641 cttgagcaga ttcgtgatga actggcagcc aacaataaag atccgcggcc gctcccttta
    14701 gtgagggtta attggccgca taacttcgta taatgtatgc tatacgaagt tatgggtacc
    14761 gggg
//
