LOCUS       sequence-131056-         762 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of S727A-STAT3. Please note that this plasmid 
            contains S727A-STAT3 tagged with FLAG, not GFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Dasgupta M, Dermawan JK, Willard B, Stark GR
  TITLE     STAT3-driven transcription depends upon the dimethylation of K49 by 
            EZH2.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Mar 31;112(13):3985-90. doi: 
            10.1073/pnas.1503152112. Epub 2015 Mar 12.
  PUBMED    25767098
REFERENCE   2  (bases 1 to 762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1503152112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-03-31- 31"; volume: "112"; issue: "13"; pages: "3985-90"
COMMENT     Sequence Label: pLEGFP-S727A-STAT3
FEATURES             Location/Qualifiers
     source          1..762
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      11..20
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(75..98)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cccttgctca ccatggtggc gaccggtgga tcccgggccc gcggtaccgt cgactgcaga
       61 attcgaagct ttcacttgtc atcgtcgtcc ttgtagtcca tgggggaggt agcgcactcc
      121 gaggtcaact ccatgtcaaa ggtgagggac tcaaactgcc ctcctgctga gggttcagca
      181 ccttcaccat tatttccaaa ctgcatcaat gaatctaaag tgcggggggc catcggcagg
      241 tcaatggtat tgctgcaggt cgttggtgtc acacagataa acttggtctt caggtatggg
      301 gcagcgctac ctgggtcagc ttcaggatgc tcctggctct ctggccgaca atactttccg
      361 aatgcctcct ccttgggaat gtcaggatag agatagacca gtggagacac caggatattg
      421 gtagcatcca tgatcttata gcccatgatg atttcagcaa atgacatgtt gttcagctgc
      481 tgctttgtgt atggttccac ggactggatc tgggtcttac cgctgatgtc cttctccacc
      541 caagtgaaag tgacgcctcc ttctttgctg ctttcactga atcttagcag gaaggtgcct
      601 ggaggcttag tgctcaagat ggcccgctcc cgctccttac tgataaagcc catgatgtac
      661 ccttcgttcc aaagggccag gatgtacttt ttcacaaggt caatgatatt gtccagccag
      721 acccagaagg agaagccctt gccagccatg ttttctttgc aa
//
