LOCUS       sequence-131057-       10672 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Generating transgenic flies for 4-hydroxytamoxifen-inducible DamID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10672)
  AUTHORS   Pindyurin AV, Pagie L, Kozhevnikova EN, van Arensbergen J, van 
            Steensel B
  TITLE     Inducible DamID systems for genomic mapping of chromatin proteins in
            Drosophila.
  JOURNAL   Nucleic Acids Res. 2016 Jul 8;44(12):5646-57. doi: 
            10.1093/nar/gkw176. Epub 2016 Mar 21.
  PUBMED    27001518
REFERENCE   2  (bases 1 to 10672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkw176"; journalName: "Nucleic Acids Res"; date: 
            "2016-07-8- 8"; volume: "44"; issue: "12"; pages: "5646-57"
COMMENT     Sequence Label: p-attB-FL.hsp70P-Dam[4-HT-intein@L127C]Myc[open]
FEATURES             Location/Qualifiers
     source          1..10672
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    7..40
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        42..494
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     441..462
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             2593..2622
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     intron          2744..2809
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2939..2959
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3231..3365
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3268..3287)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3322..3341
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    3394..3463
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(3682..3700)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3721..3737)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3721..3737)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3734..3756)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3745..3761
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3769..3775,3776..3793,3794..3799))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3814..3835
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3952..3969)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4123..4711)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4203..4222)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4882..5673,5674..5742))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5505..5524
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5743..5847)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5874..6329
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5961..5980)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6171..6192
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6455..6477
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6469..6486
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6470..6486
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6493..6512
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6493..6511
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gene            6536..10672
                     /gene="white"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
     primer_bind     complement(6545..6563)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(7015..7086,7488..7761,7836..8490,8552..8867,9088..9219,
                     9290..9904)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
ORIGIN
        1 gctagcataa cttcgtataa tgtatgctat acgaagttat cctagaatcc caaaacaaac
       61 tggttattgt ggtaggtcat ttgtttggca gaaagaaaac tcgagaaatt tctctggccg
      121 ttattcgtta ttctctcttt tctttttggg tctctccctc tctgcactaa tgctctctca
      181 ctctgtcaca cagtaaacgg catactgctc tcgttggttc gagagagcgc gcctcgaatg
      241 ttcgcgaaaa gagcgccgga gtataaatag aggcgcttcg tctacggagc gacaattcaa
      301 ttcaaacaag caaagtgaac acgtcgctaa gcgaaagcta agcaaataaa caagcgcagc
      361 tgaacaagct aaacaatctg cagtaaagtg caagttaaag tgaatcaatt aaaagtaacc
      421 agcaaccaag taaatcaact gcaactactg aaatctgcca agaagtaatt attgaataca
      481 agaagagaac tctgaatagg gaattgggaa ttcaacatga agaaaaatcg cgcttttttg
      541 aagtgggcag ggggcaagta tcccctgctt gatgatatta aacggcattt gcccaagggc
      601 gaatgtctgg ttgagccttt tgtaggtgcc gggtcggtgt ttctcaacac cgacttttct
      661 cgttatatcc ttgccgatat caatagcgac ctgatcagtc tctataacat tgtgaagatg
      721 cgtactgatg agtacgtaca ggccgcacgc gagctgtttg ttcccgaaac aaattgcgcc
      781 gaggtttact atcagttccg cgaagagttc aacaaaagcc aggatccgtt ccgtcgggcg
      841 gtactgtttt tatatttgaa ccgctacggt tacaacggcc tgtgtcgtta caattgcctt
      901 gccgagggta cccgaatctt cgatccggtc actggtacaa cgcatcgcat cgaggatgtt
      961 gtcgatgggc gcaagcctat tcatgtcgtg gctgttgcca aggacggaac gctgctcgcg
     1021 cggcccgtgg tgtcctggtt cgaccaggga acgcgggatg tgatcgggtt gcggatcgcc
     1081 ggtggcgcca tcgtgtgggc gacacccgat cacaaggtgc tgacagagta cggctggcgt
     1141 gccgccgggg aactccgcaa gggagacagg gtggccggac cgggtggttc tggtaacagc
     1201 ctggccttgt ccctgacggc cgaccagatg gtcagtgcct tgttggatgc tgagcccccc
     1261 atactctatt ccgagtatga tcctaccagt cccttcagtg aagcttcgat gatgggctta
     1321 ctgaccaacc tggcagacag ggagctggtt cacatgatca actgggcgaa gagggtgcca
     1381 ggctttgtgg atttgaccct ccatgatcag gcccaccttc tagaatgtgc ctggctagag
     1441 atcctgatga ttggtctcgt ctggcgctcc atggagcacc cagggaagct actgtttgct
     1501 cctaacttgc tcttggacag gaaccaggga aaatgtgtag agggcatggt ggagatcttc
     1561 gacatgctgc tggctacatc atctcggttc cgcatgatga atctgcaggg agaggagttt
     1621 gtgtgcctca aatctattat tttgcttaat tctggagtgt acacatttct gtccagcacc
     1681 ctgaagtctc tggaagagaa ggaccatatc caccgagccc tggacaagat cacggacact
     1741 ttgatccacc tgatggccaa ggcaggcctg accctgcagc agcagcacca gcggctggcc
     1801 cagctcctcc tcatcctctc ccacatcagg cacatgagta acaaaggaat ggagcatctg
     1861 tacagcatga agtacacgaa cgtggtgccc ctctatgacc tgctgctgga gatgctggac
     1921 gcccaccgcc tacatgcggg tggttctggt gctagccgcg tgcaggcgtt cgcggatgcc
     1981 ctggatgaca aattcctgca cgacatgctg gcggaagaac tccgctattc cgtgatccga
     2041 gaagtgctgc caacgcggcg ggcacgaacg ttcgacctcg aggtcgagga actgcacacc
     2101 ctcgtcgccg aaggggttgt cgtgcacaac tgtcgcggtg agtttaacgt gccgttcggc
     2161 cgctacaaaa aaccctattt cccggaagca gagttgtatc acttcgctga aaaagcgcag
     2221 aatgcctttt tctattgtga gtcttacgcc gatagcatgg cgcgcgcaga tgatgcatcc
     2281 gtcgtctatt gcgatccgcc ttatgcaccg ctgtctgcga ccgccaactt tacggcgtat
     2341 cacacaaaca gttttacgct tgaacaacaa gcgcatctgg cggagatcgc cgaaggtctg
     2401 gttgagcgcc atattccagt gctgatctcc aatcacgata cgatgttaac gcgtgagtgg
     2461 tatcagcgcg caaaattgca tgtcgtcaaa gttcgacgca gtataagcag caacggcggc
     2521 acacgtaaaa aggtggacga actgctggct ttgtacaaac caggagtcgt ttcacccgcg
     2581 aaaaaagccg gtgaacagaa actcatctct gaagaggatc tgggcgcgcc atttaaatgg
     2641 ccggccgcga tcgcggccgc tctagaggat ctttgtgaag gaaccttact tctgtggtgt
     2701 gacataattg gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt
     2761 aagtgtataa tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta
     2821 tggaactgat gaatgggagc agtggtggaa tgcctttaat gaggaaaacc tgttttgctc
     2881 agaagaaatg ccatctagtg atgatgaggc tactgctgac tctcaacatt ctactcctcc
     2941 aaaaaagaag agaaaggtag aagaccccaa ggactttcct tcagaattgc taagtttttt
     3001 gagtcatgct gtgtttagta atagaactct tgcttgcttt gctatttaca ccacaaagga
     3061 aaaagctgca ctgctataca agaaaattat ggaaaaatat ttgatgtata gtgccttgac
     3121 tagagatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     3181 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     3241 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     3301 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     3361 ggatccacta gtgtcgacga tgtaggtcac ggtctcgaag ccgcggtgcg ggtgccaggg
     3421 cgtgcccttg ggctccccgg gcgcgtactc cacctcaccc atctggtcca tcatgatgaa
     3481 cgggtcgagg tggcggtagt tgatcccggc gaacgcgcgg cgcaccggga agccctcgcc
     3541 ctcgaaaccg ctgggcgcgg tggtcacggt gagcacggga cgtgcgacgg cgtcggcggg
     3601 tgcggatacg cggggcagcg tcagcgggtt ctcgacggtc acggcgggca tgtcgacact
     3661 agttctagcc agcttttgtt ccctttagtg agggttaatt tcgagcttgg cgtaatcatg
     3721 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     3781 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     3841 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     3901 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     3961 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     4021 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     4081 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     4141 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     4201 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     4261 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     4321 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     4381 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     4441 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     4501 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     4561 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     4621 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     4681 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     4741 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     4801 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     4861 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     4921 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     4981 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     5041 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     5101 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     5161 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     5221 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     5281 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     5341 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     5401 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     5461 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     5521 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     5581 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     5641 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     5701 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     5761 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     5821 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgcgcc
     5881 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     5941 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     6001 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     6061 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     6121 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     6181 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     6241 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     6301 ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg caactgttgg
     6361 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
     6421 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
     6481 gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg ggcccctagt
     6541 atgtatgtaa gttaataaaa cccatttttg cggaaagtag ataaaaaaaa catttttttt
     6601 ttttactgca ctggatatca ttgaacttat ctgatcagtt ttaaatttac ttcgatccaa
     6661 gggtatttga tgtaccaggt tctttcgatt acctctcact caaaatgaca ttccactcaa
     6721 agtcagcgct gtttgcctcc ttctctgtcc acagaaatat cgccgtctct ttcgccgctg
     6781 cgtccgctat ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt ccgccttcag
     6841 ttgcattttg tcagcggttt cgtgacgaag ctccaagcgg tttacgccat caattaaaca
     6901 caaagtgctg tgccaaaact cctctcgctt cttatttttg tttgtttttt gagtgattgg
     6961 ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc ggcaatgggc
     7021 caagaggatc aggagctatt aattcgcgga ggcagcaaac acccatctgc cgagcatctg
     7081 aacaatgtga gtagtacatg tgcatacatc ttaagttcac ttgatctata ggaactgcga
     7141 ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc caaaccgcaa
     7201 ttgcacaaac aaatagtgac acgaaacaga ttattctggt agctgttctc gctatataag
     7261 acaatttttg agatcatatc atgatcaaga catctaaagg cattcatttt cgactatatt
     7321 cttttttaca aaaaatataa caaccagata ttttaagctg atcctagatg cacaaaaaat
     7381 aaataaaagt ataaacctac ttcgtaggat acttcggggt actttttgtt cggggttaga
     7441 tgagcataac gcttgtagtt gatatttgag atcccctatc attgcagggt gacagcggag
     7501 cggcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac ggcacgctcc
     7561 ggccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc gagaacctca
     7621 cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc tccggatggc
     7681 ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata ccggcgccca
     7741 ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct gtgtgaaatc
     7801 ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct atccgggcga
     7861 acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga atgcccttgc
     7921 ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc tcaatggcca
     7981 acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg atgacctctt
     8041 tatcggctcc ctaacggcca gggaacacct gattttccag gccatggtgc ggatgccacg
     8101 acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg agctttcgct
     8161 cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc tgtccggcgg
     8221 agaaaggaag cgtctggcat tcgcctccga ggcactaacc gatccgccgc ttctgatctg
     8281 cgatgagccc acctccggac tggactcatt taccgcccac agcgtcgtcc aggtgctgaa
     8341 gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt cttccgagct
     8401 gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt tcttgggcac
     8461 tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt aagggtatct
     8521 agcattacat tacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct accaactaca
     8581 atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag atcgagtccc
     8641 gtgatcggat cgccaagata tgcgacaatt ttgctattag caaagtagcc cgggatatgg
     8701 agcagttgtt ggccaccaaa aatttggaga agccactgga gcagccggag aatgggtaca
     8761 cctacaaggc cacctggttc atgcagttcc gggcggtcct gtggcgatcc tggctgtcgg
     8821 tgctcaagga accactcctc gtaaaagtgc gacttattca gacaacggtg agtggttcca
     8881 gtggaaacaa atgatataac gcttacaatt cttggaaaca aattcgctag attttagtta
     8941 gaattgcctg attccacacc cttcttagtt tttttcaatg agatgtatag tttatagttt
     9001 tgcagaaaat aaataaattt catttaactc gcgaacatgt tgaagatatg aatattaatg
     9061 agatgcgagt aacattttaa tttgcagatg gttgccatct tgattggcct catctttttg
     9121 ggccaacaac tcacgcaagt gggcgtgatg aatatcaacg gagccatctt cctcttcctg
     9181 accaacatga cctttcaaaa cgtctttgcc acgataaatg taagtcttgt ttagaataca
     9241 tttgcatatt aataatttac taactttcta atgaatcgat tcgatttagg tgttcacctc
     9301 agagctgcca gtttttatga gggaggcccg aagtcgactt tatcgctgtg acacatactt
     9361 tctgggcaaa acgattgccg aattaccgct ttttctcaca gtgccactgg tcttcacggc
     9421 gattgcctat ccgatgatcg gactgcgggc cggagtgctg cacttcttca actgcctggc
     9481 gctggtcact ctggtggcca atgtgtcaac gtccttcgga tatctaatat cctgcgccag
     9541 ctcctcgacc tcgatggcgc tgtctgtggg tccgccggtt atcataccat tcctgctctt
     9601 tggcggcttc ttcttgaact cgggctcggt gccagtatac ctcaaatggt tgtcgtacct
     9661 ctcatggttc cgttacgcca acgagggtct gctgattaac caatgggcgg acgtggagcc
     9721 gggcgaaatt agctgcacat cgtcgaacac cacgtgcccc agttcgggca aggtcatcct
     9781 ggagacgctt aacttctccg ccgccgatct gccgctggac tacgtgggtc tggccattct
     9841 catcgtgagc ttccgggtgc tcgcatatct ggctctaaga cttcgggccc gacgcaagga
     9901 gtagccgaca tatatccgaa ataactgctt gttttttttt tttaccatta ttaccatcgt
     9961 gtttactgtt tattgccccc tcaaaaagct aatgtaatta tatttgtgcc aataaaaaca
    10021 agatatgacc tatagaatac aagtatttcc ccttcgaaca tccccacaag tagactttgg
    10081 atttgtcttc taaccaaaag acttacacac ctgcatacct tacatcaaaa actcgtttat
    10141 cgctacataa aacaccggga tatatttttt atatacatac ttttcaaatc gcgcgccctc
    10201 ttcataattc acctccacca caccacgttt cgtagttgct ctttcgctgt ctcccacccg
    10261 ctctccgcaa cacattcacc ttttgttcga cgaccttgga gcgactgtcg ttagttccgc
    10321 gcgattcggt tcgctcaaat ggttccgagt ggttcatttc gtctcaatag aaattagtaa
    10381 taaatatttg tatgtacaat ttatttgctc caatatattt gtatatattt ccctcacagc
    10441 tatatttatt ctaatttaat attatgactt tttaaggtaa ttttttgtga cctgttcgga
    10501 gtgattagcg ttacaatttg aactgaaagt gacatccagt gtttgttcct tgtgtagatg
    10561 catctcaaaa aaatggtggg cataatagtg ttgtttatat atatcaaaaa taacaactat
    10621 aataataaga atacatttaa tttagaaaat gcttggattt cactggaact ag
//
