LOCUS       sequence-131059-         760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of K49R-STAT3. Please note that this plasmid 
            contains K49R-STAT3 tagged with FLAG, not GFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Dasgupta M, Dermawan JK, Willard B, Stark GR
  TITLE     STAT3-driven transcription depends upon the dimethylation of K49 by 
            EZH2.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Mar 31;112(13):3985-90. doi: 
            10.1073/pnas.1503152112. Epub 2015 Mar 12.
  PUBMED    25767098
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1503152112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-03-31- 31"; volume: "112"; issue: "13"; pages: "3985-90"
COMMENT     Sequence Label: pLEGFP-K49R-STAT3
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      9..18
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(73..96)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cttgctcacc atggtggcga ccggtggatc ccgggcccgc ggtaccgtcg actgcagaat
       61 tcgaagcttt cacttgtcat cgtcgtcctt gtagtccatg ggggaggtag cgcactccga
      121 ggtcaactcc atgtcaaagg tgagggactc aaactgccct cctgctgagg gttcagcacc
      181 ttcaccatta tttccaaact gcatcaatga atctaaagtg cggggggaca tcggcaggtc
      241 aatggtattg ctgcaggtcg ttggtgtcac acagataaac ttggtcttca ggtatggggc
      301 agcgctacct gggtcagctt caggatgctc ctggctctct ggccgacaat actttccgaa
      361 tgcctcctcc ttgggaatgt caggatagag atagaccagt ggagacacca ggatattggt
      421 agcatccatg atcttatagc ccatgatgat ttcagcaaat gacatgttgt tcagctgctg
      481 ctttgtgtat ggttccacgg actggatctg ggtcttaccg ctgatgtcct tctccaccca
      541 agtgaaagtg acgcctcctt ctttgctgct ttcactgaat cttagcagga aggtgcctgg
      601 aggcttagtg ctcaagatgg cccgctcccg ctccttactg ataaagccca tgatgtaccc
      661 ttcgttccaa agggccagga tgtacttttt cacaaggtca atgatattgt ccagccagac
      721 ccagaaggag aagcccttgc cagccatgtt ttctttgcaa
//
