LOCUS       sequence-13106-e        4278 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4278)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 4278)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1459-HQ858750
FEATURES             Location/Qualifiers
     source          1..4278
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             112..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(1835..1934)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1951..1970)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1952..1970)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1975..1991)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1975..1991)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2093..2112)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2104..2913
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2171..2190)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3060..3648
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3549..3568
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3802..3819
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3978..4005)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4097..4183)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4232..4254
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4246..4263
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4247..4263
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc cttgtttaac
      121 tttaagaagg agcccttcac catgtaccgg gtgaagagcg agggggaggg cgagggcgag
      181 ggcgactgcg aaatgatgct gcaggaacag atggactcgc tggtggccga cgacgtcagc
      241 agcggaggag ggtcgcctca caggggcgtc ggcacgcccc tgaagaaggg gccatggacg
      301 tccgcggagg acgccatcct ggtggactac gttaagaaga acggcgaggg caactggaac
      361 gcggtgcaga agaacaccgg gctgttccgc tgcggcaaga gctgccgcct ccggtgggcg
      421 aaccacctca ggcccaacct caagaagggg gccttcaccc ccgaggagga gcgcctcatc
      481 atccagctcc acgccaagat ggggaacaag tgggcgagga tggctggtca cttgccaggg
      541 cgtactgaca atgagatcaa gaactactgg aacactcgaa taaagagatg tcaacgagct
      601 agccttccta tttatcctgc tagtgtatgc aatcaatcta caaatgaaga tcagcaactg
      661 tctggtaatt ttaacggtgg cgagaatata tccaatgatc ttctatctgg gaacagcctt
      721 tatctgccag attttaccag tgacaatttc attgcgaacc cagaggcttt atcctatgca
      781 ccacagttgt cagctgtttc aataagcaat ttgctcggcc aaagctttgc atcaaaaagt
      841 tgtagcttca tggatcaggt tgaccaagcg gggatgctga aacaatctgg ctgtgtgctt
      901 cctgcattga gcgatgccat tgacagtgtg ctttcctcag ctgatcattt ttcaaatgac
      961 tctgagaagc tcaggcaggc tttaggtttt gattatctga atgaagccaa tgctagcagc
     1021 aagagtattg cacctttcgg ggttgcactt actggcagcc atgccttttt aaatggcaat
     1081 ttctctgctt ctaggcccac aaatggtcct ttgaagatgg agctcccttc actccaagat
     1141 actgaatctg atccaaatag ctggctcaag tatactgtgg ctcctgcaat gcagcctact
     1201 gaattagtag atccttacct gcagtctcca tcagcgaccc cttcagtgaa atctgagtgt
     1261 gcatcgccga ggaacagtgg tcttttggaa gagctgcttc atgaagctca ggcactaaga
     1321 tctgggaaga accaacaatc atcggtccga agttcaagtt cttctgctgg cacaccttat
     1381 gagactacca cggtggttag cccagagttt gatatgggtc aggaatattg ggaagaacag
     1441 cccagttctt tcctcagtga atatgctcat tttagtggaa attctttcac tgaatccact
     1501 cctcctgtta gtgctgcgtc acctgatatc ttccagctct ccaagatttc tcctgcacaa
     1561 agcccttcaa tgggctctgg cgagcaggcg ttagagccta aacatgagtc ggcagcttca
     1621 cctcgtcctg aaaacttgag gcctgatgca ttattctctg ggaacacagc cgatccatcc
     1681 attttcaata atgccatagc catgctcctg ggcaatggca ttgatgccga gtacaaacct
     1741 gttcttggtg atggaattgt gctcgattct tcgtcatgga acaacatgca acatgctttt
     1801 cagatggcgg gattcaaaaa gggtgggcgc gccgacccag ctttcttgta caaagttggc
     1861 attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat
     1921 aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata
     1981 gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat
     2041 aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt
     2101 gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct
     2161 gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat
     2221 cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt
     2281 gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt
     2341 ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc
     2401 cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt
     2461 gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt
     2521 aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt
     2581 gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa
     2641 atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt
     2701 gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga
     2761 atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct
     2821 tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg
     2881 cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac
     2941 tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa atcccttaac
     3001 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3061 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3121 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3181 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     3241 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3301 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3361 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3421 tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg
     3481 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3541 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3601 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3661 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3721 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3781 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3841 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3901 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3961 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     4021 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4081 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4141 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4201 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4261 agtcttaagc tcgggccc
//
