LOCUS       sequence-131062-       11797 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Generating transgenic flies for FLP-inducible DamID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11797)
  AUTHORS   Pindyurin AV, Pagie L, Kozhevnikova EN, van Arensbergen J, van 
            Steensel B
  TITLE     Inducible DamID systems for genomic mapping of chromatin proteins in
            Drosophila.
  JOURNAL   Nucleic Acids Res. 2016 Jul 8;44(12):5646-57. doi: 
            10.1093/nar/gkw176. Epub 2016 Mar 21.
  PUBMED    27001518
REFERENCE   2  (bases 1 to 11797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkw176"; journalName: "Nucleic Acids Res"; date: 
            "2016-07-8- 8"; volume: "44"; issue: "12"; pages: "5646-57"
COMMENT     Sequence Label: p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMycPC
FEATURES             Location/Qualifiers
     source          1..11797
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    7..40
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        46..284
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     231..252
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     protein_bind    304..351
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     intron          867..932
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1062..1082
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1507..1641
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    1669..1716
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             2566..2595
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3004..3033
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             3082..3105
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     intron          3869..3934
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4064..4084
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4356..4490
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    4519..4588
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(4807..4825)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4846..4862)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4846..4862)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4859..4881)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4870..4886
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4894..4900,4901..4918,4919..4924))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4939..4960
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5077..5094)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5248..5836)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5328..5347)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6007..6798,6799..6867))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6630..6649
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6868..6972)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6999..7454
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7086..7105)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7296..7317
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7580..7602
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7594..7611
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7595..7611
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7618..7637
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7618..7636
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gene            7661..11797
                     /gene="white"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
     primer_bind     complement(7670..7688)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(8140..8211,8613..8886,8961..9615,9677..9992,
                     10213..10344,10415..11029)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
ORIGIN
        1 gctagcataa cttcgtataa tgtatgctat acgaagttat gagcgccgga gtataaatag
       61 aggcgcttcg tctacggagc gacaattcaa ttcaaacaag caaagtgaac acgtcgctaa
      121 gcgaaagcta agcaaataaa caagcgcagc tgaacaagct aaacaatctg cagtaaagtg
      181 caagttaaag tgaatcaatt aaaagtaacc agcaaccaag taaatcaact gcaactactg
      241 aaatctgcca agaagtaatt attgaataca agaagagaac tctgaatagg gaattgggaa
      301 ttcgaagttc ctattccgaa gttcctattc tctagaaagt ataggaactt ctcggggaca
      361 ccaaatatgg cgatctcggc cttttcgttt cttggagctg ggacatgttt gccatcgatc
      421 catctaccac cagaacggcc gttagatctg ctgccaccgt tgtttccacc gaagaaacca
      481 ccgttgccgt aaccaccacg acggttgttg ctaaagaagc tgccaccgcc acggccaccg
      541 ttgtagccgc cgttgttgtt attgtagttg ctactgttat ttctggcact tcttggtttt
      601 cctcttaagt gaggaggaac ataaccattc tcgttgttgt cgttgatgct taaattttgc
      661 acttgttcgc tcagttcagc cataatatga aatgcttttc ttgttgttct tacggaatac
      721 cacttgccac ctatcaccac aactaacttt ttcccgttcc tccatctctt ttatattttt
      781 tttctcgagg gatctttgtg aaggaacctt acttctgtgg tgtgacataa ttggacaaac
      841 tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta taatgtgtta
      901 aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact gatgaatggg
      961 agcagtggtg gaatgccttt aatgaggaaa acctgttttg ctcagaagaa atgccatcta
     1021 gtgatgatga ggctactgct gactctcaac attctactcc tccaaaaaag aagagaaagg
     1081 tagaagaccc caaggacttt ccttcagaat tgctaagttt tttgagtcat gctgtgttta
     1141 gtaatagaac tcttgcttgc tttgctattt acaccacaaa ggaaaaagct gcactgctat
     1201 acaagaaaat tatggaaaaa tattctgtaa cctttataag taggcataac agttataatc
     1261 ataacatact gttttttctt actccacaca ggcatagagt gtctgctatt aataactatg
     1321 ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg ggttaataag gaatatttga
     1381 tgtatagtgc cttgactaga gatcataatc agccatacca catttgtaga ggttttactt
     1441 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     1501 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     1561 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     1621 gtatcttatc atgtctggat ctgacatggt aagtaagctt gggctgcaga agttcctatt
     1681 ccgaagttcc tattctctag aaagtatagg aacttcgaat tcaacatgaa gaaaaatcgc
     1741 gcttttttga agtgggcagg gggcaagtat cccctgcttg atgatattaa acggcatttg
     1801 cccaagggcg aatgtctggt tgagcctttt gtaggtgccg ggtcggtgtt tctcaacacc
     1861 gacttttctc gttatatcct tgccgatatc aatagcgacc tgatcagtct ctataacatt
     1921 gtgaagatgc gtactgatga gtacgtacag gccgcacgcg agctgtttgt tcccgaaaca
     1981 aattgcgccg aggtttacta tcagttccgc gaagagttca acaaaagcca ggatccgttc
     2041 cgtcgggcgg tactgttttt atatttgaac cgctacggtt acaacggcct gtgtcgttac
     2101 aatctgcgcg gtgagtttaa cgtgccgttc ggccgctaca aaaaacccta tttcccggaa
     2161 gcagagttgt atcacttcgc tgaaaaagcg cagaatgcct ttttctattg tgagtcttac
     2221 gccgatagca tggcgcgcgc agatgatgca tccgtcgtct attgcgatcc gccttatgca
     2281 ccgctgtctg cgaccgccaa ctttacggcg tatcacacaa acagttttac gcttgaacaa
     2341 caagcgcatc tggcggagat cgccgaaggt ctggttgagc gccatattcc agtgctgatc
     2401 tccaatcacg atacgatgtt aacgcgtgag tggtatcagc gcgcaaaatt gcatgtcgtc
     2461 aaagttcgac gcagtataag cagcaacggc ggcacacgta aaaaggtgga cgaactgctg
     2521 gctttgtaca aaccaggagt cgtttcaccc gcgaaaaaag ccggtgaaca gaaactcatc
     2581 tctgaagagg atctgggcgc gcctatgact ggtcgaggca aggggagtaa ggggaagttg
     2641 gggcgcgaca atgcgaccga cgatccagtc gatctagtgt acgcggctga gaaaatcatc
     2701 caaaagcgcg ttaagaaggg cgtcgtggag taccgtgtca agtggaaggg ctggaaccag
     2761 cgctacaaca cctgggaacc ggaggtaaac atcctggatc gccgcctcat cgacatctac
     2821 gaacaaacga acaaatcctc cggaactccc tccaagcgag gcattaagaa gaaggagaaa
     2881 gaacccgatc cggagccgga atccgaggag gatgagtaca ccttcaccga aaacgatgtg
     2941 gacacgcatc aagccaccac ctcatcggct acccacgata aggagtcgaa gaaggagaag
     3001 aagcaccatc accaccacca ccatcatcac cacatcaagt ccgaacgcaa cagtggacgg
     3061 cgctcggaat ctccgctgac ccaccatcat catcaccacc accacgagtc caagcgtcag
     3121 cgcattgatc acagctcctc ctcgaacagc agcttcacgc acaactcatt tgtccccgag
     3181 ccggacagca actcctccag ctccgaggat cagccgctga tcggcaccaa acgcaaagcc
     3241 gaggtgctca aggaatcggg caagattgga gttacgatta agacctcccc agatggcccc
     3301 actattaagc ctcagccgac ccagcaggta actcccagcc agcagcagcc cttccaggat
     3361 cagcagcaag cggaaaagat tgccagcgag gctgcaacgc agctgaaatc tgagcagcag
     3421 gccaccccac tggccacaga agccataaac acaacgcccg cagagtccgg agctgaggaa
     3481 gaagaagtag ccaacgagga aggcaaccaa caggcgccac aggttccttc cgagaacaat
     3541 aacataccaa aaccgtgcaa caacctggct atcaaccaga aacagccgct tactcctctt
     3601 tcgccgcgtg cccttccgcc gcgcttctgg ctgccggcca aatgcaacat atccaaccgg
     3661 gtggtgatca cagacgtcac cgtaaacctg gagaccgtta ccatacgcga gtgcaagacg
     3721 gagcgcgggt tttttcgtga gcgcgacatg aaaggcgatt cgtcgccagt agcttgagcg
     3781 gccgctctag aggatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa
     3841 actacctaca gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt
     3901 taaactactg attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg
     3961 ggagcagtgg tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc
     4021 tagtgatgat gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa
     4081 ggtagaagac cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt
     4141 tagtaataga actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct
     4201 atacaagaaa attatggaaa aatatttgat gtatagtgcc ttgactagag atcataatca
     4261 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     4321 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     4381 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     4441 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cactagtgtc
     4501 gacgatgtag gtcacggtct cgaagccgcg gtgcgggtgc cagggcgtgc ccttgggctc
     4561 cccgggcgcg tactccacct cacccatctg gtccatcatg atgaacgggt cgaggtggcg
     4621 gtagttgatc ccggcgaacg cgcggcgcac cgggaagccc tcgccctcga aaccgctggg
     4681 cgcggtggtc acggtgagca cgggacgtgc gacggcgtcg gcgggtgcgg atacgcgggg
     4741 cagcgtcagc gggttctcga cggtcacggc gggcatgtcg acactagttc tagccagctt
     4801 ttgttccctt tagtgagggt taatttcgag cttggcgtaa tcatggtcat agctgtttcc
     4861 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     4921 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     4981 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     5041 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     5101 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     5161 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     5221 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     5281 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     5341 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     5401 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     5461 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     5521 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     5581 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     5641 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     5701 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     5761 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     5821 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     5881 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     5941 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     6001 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     6061 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     6121 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     6181 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     6241 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     6301 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     6361 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     6421 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     6481 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     6541 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     6601 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     6661 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     6721 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     6781 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     6841 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     6901 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     6961 aggggttccg cgcacatttc cccgaaaagt gccacctgac gcgccctgta gcggcgcatt
     7021 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     7081 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     7141 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     7201 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     7261 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     7321 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     7381 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     7441 aacgcttaca atttccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     7501 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     7561 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa
     7621 tacgactcac tatagggcga attgggtacg taccgggccc ctagtatgta tgtaagttaa
     7681 taaaacccat ttttgcggaa agtagataaa aaaaacattt ttttttttta ctgcactgga
     7741 tatcattgaa cttatctgat cagttttaaa tttacttcga tccaagggta tttgatgtac
     7801 caggttcttt cgattacctc tcactcaaaa tgacattcca ctcaaagtca gcgctgtttg
     7861 cctccttctc tgtccacaga aatatcgccg tctctttcgc cgctgcgtcc gctatctctt
     7921 tcgccaccgt ttgtagcgtt acgtagcgtc aatgtccgcc ttcagttgca ttttgtcagc
     7981 ggtttcgtga cgaagctcca agcggtttac gccatcaatt aaacacaaag tgctgtgcca
     8041 aaactcctct cgcttcttat ttttgtttgt tttttgagtg attggggtgg tgattggttt
     8101 tgggtgggta agcaggggaa agtgtgaaaa atcccggcaa tgggccaaga ggatcaggag
     8161 ctattaattc gcggaggcag caaacaccca tctgccgagc atctgaacaa tgtgagtagt
     8221 acatgtgcat acatcttaag ttcacttgat ctataggaac tgcgattgca acatcaaatt
     8281 gtctgcggcg tgagaactgc gacccacaaa aatcccaaac cgcaattgca caaacaaata
     8341 gtgacacgaa acagattatt ctggtagctg ttctcgctat ataagacaat ttttgagatc
     8401 atatcatgat caagacatct aaaggcattc attttcgact atattctttt ttacaaaaaa
     8461 tataacaacc agatatttta agctgatcct agatgcacaa aaaataaata aaagtataaa
     8521 cctacttcgt aggatacttc ggggtacttt ttgttcgggg ttagatgagc ataacgcttg
     8581 tagttgatat ttgagatccc ctatcattgc agggtgacag cggagcggct tcgcagagct
     8641 gcattaacca gggcttcggg caggccaaaa actacggcac gctccggcca cccagtccgc
     8701 cggaggactc cggttcaggg agcggccaac tagccgagaa cctcacctat gcctggcaca
     8761 atatggacat ctttggggcg gtcaatcagc cgggctccgg atggcggcag ctggtcaacc
     8821 ggacacgcgg actattctgc aacgagcgac acataccggc gcccaggaaa catttgctca
     8881 agaacggtga gtttctattc gcagtcggct gatctgtgtg aaatcttaat aaagggtcca
     8941 attaccaatt tgaaactcag tttgcggcgt ggcctatccg ggcgaacttt tggccgtgat
     9001 gggcagttcc ggtgccggaa agacgaccct gctgaatgcc cttgcctttc gatcgccgca
     9061 gggcatccaa gtatcgccat ccgggatgcg actgctcaat ggccaacctg tggacgccaa
     9121 ggagatgcag gccaggtgcg cctatgtcca gcaggatgac ctctttatcg gctccctaac
     9181 ggccagggaa cacctgattt tccaggccat ggtgcggatg ccacgacatc tgacctatcg
     9241 gcagcgagtg gcccgcgtgg atcaggtgat ccaggagctt tcgctcagca aatgtcagca
     9301 cacgatcatc ggtgtgcccg gcagggtgaa aggtctgtcc ggcggagaaa ggaagcgtct
     9361 ggcattcgcc tccgaggcac taaccgatcc gccgcttctg atctgcgatg agcccacctc
     9421 cggactggac tcatttaccg cccacagcgt cgtccaggtg ctgaagaagc tgtcgcagaa
     9481 gggcaagacc gtcatcctga ccattcatca gccgtcttcc gagctgtttg agctctttga
     9541 caagatcctt ctgatggccg agggcagggt agctttcttg ggcactccca gcgaagccgt
     9601 cgacttcttt tcctagtgag ttcgatgtgt ttattaaggg tatctagcat tacattacat
     9661 ctcaactcct atccagcgtg ggtgcccagt gtcctaccaa ctacaatccg gcggactttt
     9721 acgtacaggt gttggccgtt gtgcccggac gggagatcga gtcccgtgat cggatcgcca
     9781 agatatgcga caattttgct attagcaaag tagcccggga tatggagcag ttgttggcca
     9841 ccaaaaattt ggagaagcca ctggagcagc cggagaatgg gtacacctac aaggccacct
     9901 ggttcatgca gttccgggcg gtcctgtggc gatcctggct gtcggtgctc aaggaaccac
     9961 tcctcgtaaa agtgcgactt attcagacaa cggtgagtgg ttccagtgga aacaaatgat
    10021 ataacgctta caattcttgg aaacaaattc gctagatttt agttagaatt gcctgattcc
    10081 acacccttct tagttttttt caatgagatg tatagtttat agttttgcag aaaataaata
    10141 aatttcattt aactcgcgaa catgttgaag atatgaatat taatgagatg cgagtaacat
    10201 tttaatttgc agatggttgc catcttgatt ggcctcatct ttttgggcca acaactcacg
    10261 caagtgggcg tgatgaatat caacggagcc atcttcctct tcctgaccaa catgaccttt
    10321 caaaacgtct ttgccacgat aaatgtaagt cttgtttaga atacatttgc atattaataa
    10381 tttactaact ttctaatgaa tcgattcgat ttaggtgttc acctcagagc tgccagtttt
    10441 tatgagggag gcccgaagtc gactttatcg ctgtgacaca tactttctgg gcaaaacgat
    10501 tgccgaatta ccgctttttc tcacagtgcc actggtcttc acggcgattg cctatccgat
    10561 gatcggactg cgggccggag tgctgcactt cttcaactgc ctggcgctgg tcactctggt
    10621 ggccaatgtg tcaacgtcct tcggatatct aatatcctgc gccagctcct cgacctcgat
    10681 ggcgctgtct gtgggtccgc cggttatcat accattcctg ctctttggcg gcttcttctt
    10741 gaactcgggc tcggtgccag tatacctcaa atggttgtcg tacctctcat ggttccgtta
    10801 cgccaacgag ggtctgctga ttaaccaatg ggcggacgtg gagccgggcg aaattagctg
    10861 cacatcgtcg aacaccacgt gccccagttc gggcaaggtc atcctggaga cgcttaactt
    10921 ctccgccgcc gatctgccgc tggactacgt gggtctggcc attctcatcg tgagcttccg
    10981 ggtgctcgca tatctggctc taagacttcg ggcccgacgc aaggagtagc cgacatatat
    11041 ccgaaataac tgcttgtttt ttttttttac cattattacc atcgtgttta ctgtttattg
    11101 ccccctcaaa aagctaatgt aattatattt gtgccaataa aaacaagata tgacctatag
    11161 aatacaagta tttccccttc gaacatcccc acaagtagac tttggatttg tcttctaacc
    11221 aaaagactta cacacctgca taccttacat caaaaactcg tttatcgcta cataaaacac
    11281 cgggatatat tttttatata catacttttc aaatcgcgcg ccctcttcat aattcacctc
    11341 caccacacca cgtttcgtag ttgctctttc gctgtctccc acccgctctc cgcaacacat
    11401 tcaccttttg ttcgacgacc ttggagcgac tgtcgttagt tccgcgcgat tcggttcgct
    11461 caaatggttc cgagtggttc atttcgtctc aatagaaatt agtaataaat atttgtatgt
    11521 acaatttatt tgctccaata tatttgtata tatttccctc acagctatat ttattctaat
    11581 ttaatattat gactttttaa ggtaattttt tgtgacctgt tcggagtgat tagcgttaca
    11641 atttgaactg aaagtgacat ccagtgtttg ttccttgtgt agatgcatct caaaaaaatg
    11701 gtgggcataa tagtgttgtt tatatatatc aaaaataaca actataataa taagaataca
    11761 tttaatttag aaaatgcttg gatttcactg gaactag
//
