LOCUS       sequence-131064-       12493 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Generating transgenic flies for FLP-inducible DamID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12493)
  AUTHORS   Pindyurin AV, Pagie L, Kozhevnikova EN, van Arensbergen J, van 
            Steensel B
  TITLE     Inducible DamID systems for genomic mapping of chromatin proteins in
            Drosophila.
  JOURNAL   Nucleic Acids Res. 2016 Jul 8;44(12):5646-57. doi: 
            10.1093/nar/gkw176. Epub 2016 Mar 21.
  PUBMED    27001518
REFERENCE   2  (bases 1 to 12493)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkw176"; journalName: "Nucleic Acids Res"; date: 
            "2016-07-8- 8"; volume: "44"; issue: "12"; pages: "5646-57"
COMMENT     Sequence Label: p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMycLAM
FEATURES             Location/Qualifiers
     source          1..12493
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    7..40
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        46..284
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     231..252
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     protein_bind    304..351
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     intron          867..932
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1062..1082
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1507..1641
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    1669..1716
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             2566..2595
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     intron          4565..4630
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4760..4780
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5052..5186
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    5215..5284
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(5503..5521)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5542..5558)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5542..5558)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5555..5577)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5566..5582
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5590..5596,5597..5614,5615..5620))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5635..5656
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5773..5790)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5944..6532)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6024..6043)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6703..7494,7495..7563))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7326..7345
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7564..7668)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7695..8150
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7782..7801)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7992..8013
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8276..8298
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8290..8307
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8291..8307
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8314..8333
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8314..8332
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gene            8357..12493
                     /gene="white"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
     primer_bind     complement(8366..8384)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(8836..8907,9309..9582,9657..10311,10373..10688,
                     10909..11040,11111..11725)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
ORIGIN
        1 gctagcataa cttcgtataa tgtatgctat acgaagttat gagcgccgga gtataaatag
       61 aggcgcttcg tctacggagc gacaattcaa ttcaaacaag caaagtgaac acgtcgctaa
      121 gcgaaagcta agcaaataaa caagcgcagc tgaacaagct aaacaatctg cagtaaagtg
      181 caagttaaag tgaatcaatt aaaagtaacc agcaaccaag taaatcaact gcaactactg
      241 aaatctgcca agaagtaatt attgaataca agaagagaac tctgaatagg gaattgggaa
      301 ttcgaagttc ctattccgaa gttcctattc tctagaaagt ataggaactt ctcggggaca
      361 ccaaatatgg cgatctcggc cttttcgttt cttggagctg ggacatgttt gccatcgatc
      421 catctaccac cagaacggcc gttagatctg ctgccaccgt tgtttccacc gaagaaacca
      481 ccgttgccgt aaccaccacg acggttgttg ctaaagaagc tgccaccgcc acggccaccg
      541 ttgtagccgc cgttgttgtt attgtagttg ctactgttat ttctggcact tcttggtttt
      601 cctcttaagt gaggaggaac ataaccattc tcgttgttgt cgttgatgct taaattttgc
      661 acttgttcgc tcagttcagc cataatatga aatgcttttc ttgttgttct tacggaatac
      721 cacttgccac ctatcaccac aactaacttt ttcccgttcc tccatctctt ttatattttt
      781 tttctcgagg gatctttgtg aaggaacctt acttctgtgg tgtgacataa ttggacaaac
      841 tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta taatgtgtta
      901 aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact gatgaatggg
      961 agcagtggtg gaatgccttt aatgaggaaa acctgttttg ctcagaagaa atgccatcta
     1021 gtgatgatga ggctactgct gactctcaac attctactcc tccaaaaaag aagagaaagg
     1081 tagaagaccc caaggacttt ccttcagaat tgctaagttt tttgagtcat gctgtgttta
     1141 gtaatagaac tcttgcttgc tttgctattt acaccacaaa ggaaaaagct gcactgctat
     1201 acaagaaaat tatggaaaaa tattctgtaa cctttataag taggcataac agttataatc
     1261 ataacatact gttttttctt actccacaca ggcatagagt gtctgctatt aataactatg
     1321 ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg ggttaataag gaatatttga
     1381 tgtatagtgc cttgactaga gatcataatc agccatacca catttgtaga ggttttactt
     1441 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     1501 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     1561 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     1621 gtatcttatc atgtctggat ctgacatggt aagtaagctt gggctgcaga agttcctatt
     1681 ccgaagttcc tattctctag aaagtatagg aacttcgaat tcaacatgaa gaaaaatcgc
     1741 gcttttttga agtgggcagg gggcaagtat cccctgcttg atgatattaa acggcatttg
     1801 cccaagggcg aatgtctggt tgagcctttt gtaggtgccg ggtcggtgtt tctcaacacc
     1861 gacttttctc gttatatcct tgccgatatc aatagcgacc tgatcagtct ctataacatt
     1921 gtgaagatgc gtactgatga gtacgtacag gccgcacgcg agctgtttgt tcccgaaaca
     1981 aattgcgccg aggtttacta tcagttccgc gaagagttca acaaaagcca ggatccgttc
     2041 cgtcgggcgg tactgttttt atatttgaac cgctacggtt acaacggcct gtgtcgttac
     2101 aatctgcgcg gtgagtttaa cgtgccgttc ggccgctaca aaaaacccta tttcccggaa
     2161 gcagagttgt atcacttcgc tgaaaaagcg cagaatgcct ttttctattg tgagtcttac
     2221 gccgatagca tggcgcgcgc agatgatgca tccgtcgtct attgcgatcc gccttatgca
     2281 ccgctgtctg cgaccgccaa ctttacggcg tatcacacaa acagttttac gcttgaacaa
     2341 caagcgcatc tggcggagat cgccgaaggt ctggttgagc gccatattcc agtgctgatc
     2401 tccaatcacg atacgatgtt aacgcgtgag tggtatcagc gcgcaaaatt gcatgtcgtc
     2461 aaagttcgac gcagtataag cagcaacggc ggcacacgta aaaaggtgga cgaactgctg
     2521 gctttgtaca aaccaggagt cgtttcaccc gcgaaaaaag ccggtgaaca gaaactcatc
     2581 tctgaagagg atctgggcgc gcctatgtcg agcaaatccc gacgtgctgg caccgccacg
     2641 ccgcagcccg gcaacacctc caccccccgg ccgccatcgg cgggtccgca gccgccgccg
     2701 ccgtccactc actcgcagac ggcctccagc cccctcagcc ccacccggca ctcgcgcgtg
     2761 gccgagaagg tggagctgca gaacctgaac gatcgcctgg ccacctacat tgaccgggtg
     2821 cgcaacctgg agacggagaa ctcccgcctc accatcgagg tgcagaccac cagggacacg
     2881 gtcacacgcg agaccaccaa catcaagaac atcttcgagg ccgagctgct ggagacgcgc
     2941 cgtctgctcg atgacacagc tagggatcgc gctcgtgccg agatcgatat caagcgtctc
     3001 tgggaggaga acgaggagct caagaacaag ctggacaaga agaccaagga gtgcaccact
     3061 gctgagggca atgtccgcat gtacgagtcg cgcgccaacg agctgaacaa caaatacaac
     3121 caggccaacg ccgatcggaa gaagcttaac gaagacctga atgaggcgct aaaggagctg
     3181 gagagactgc gtaagcagtt cgaggaaacg cggaagaacc tggaacagga gacactgtcg
     3241 cgcgttgacc tggagaacac cattcagagt ctgcgcgagg agctctcgtt caaggatcag
     3301 atccattcgc aggagatcaa tgagtcgcgc cgcatcaaac agacagagta tagcgagatc
     3361 gacggtcgcc tcagctccga gtacgatgcc aagttgaagc agtcgctgca ggagctgcgc
     3421 gcccagtacg aggagcagat gcagattaat cgcgatgaaa tccagtccct ctacgaggac
     3481 aagatccaac gactgcaaga ggccgccgca cgcacatcca attccacgca caagtccatc
     3541 gaggagctgc gctccactcg tgtgcgtatc gatgcgctca acgccaatat caacgaactg
     3601 gagcaagcca atgccgacct caatgcgcgg atccgtgatc tggagcgcca gctggacaac
     3661 gatcgcgaac gccacggtca agagatagac cttctcgaga aggagctcat tcggctgcgc
     3721 gaagagatga cgcaacagct caaggagtac caggacctta tggacatcaa ggtctccctg
     3781 gatttggaaa tcgccgcata cgacaagctg ctggtgggcg aggaggctcg tttgaacatc
     3841 accccagcca ccaacacggc cacagtgcag tcctttagcc agtcgctgcg caactccacg
     3901 cgagccacgc catcgcgtcg cactccctct gctgccgtga agcgcaaacg cgccgtggtc
     3961 gacgagtcgg aggatcacag cgtcgccgat tactatgtgt ccgccagtgc caagggcaac
     4021 gtggagatca aggagatcga tcccgagggc aagttcgtaa ggctgttcaa caagggcagc
     4081 gaggaggtgg ccatcggtgg ctggcagctg cagcggctaa tcaacgagaa aggtccttcg
     4141 accacttaca agttccatcg atcggtgagg atcgagccaa atggcgtgat caccgtttgg
     4201 tcggcggaca ccaaggcctc gcacgagccg ccatcgagcc ttgtgatgaa gtcacagaag
     4261 tgggtctccg ccgacaacac taggacgatt ttgctgaact ccgagggcga ggccgtggcc
     4321 aatctggatc gcatcaagcg cattgtgtcc caacacacat cctcctcccg gctgagtcgt
     4381 cgtcgcagcg tgaccgccgt ggacggcaat gagcagctct accaccagca gggcgatcct
     4441 cagcagtcaa acgagaagtg cgccattatg taagcggccg ctctagagga tctttgtgaa
     4501 ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc
     4561 taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg
     4621 tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa
     4681 tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga
     4741 ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc
     4801 ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt
     4861 tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata
     4921 tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt
     4981 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     5041 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     5101 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     5161 tcaatgtatc ttatcatgtc tggatccact agtgtcgacg atgtaggtca cggtctcgaa
     5221 gccgcggtgc gggtgccagg gcgtgccctt gggctccccg ggcgcgtact ccacctcacc
     5281 catctggtcc atcatgatga acgggtcgag gtggcggtag ttgatcccgg cgaacgcgcg
     5341 gcgcaccggg aagccctcgc cctcgaaacc gctgggcgcg gtggtcacgg tgagcacggg
     5401 acgtgcgacg gcgtcggcgg gtgcggatac gcggggcagc gtcagcgggt tctcgacggt
     5461 cacggcgggc atgtcgacac tagttctagc cagcttttgt tccctttagt gagggttaat
     5521 ttcgagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     5581 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     5641 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     5701 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     5761 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     5821 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     5881 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     5941 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     6001 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     6061 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     6121 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     6181 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     6241 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     6301 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     6361 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     6421 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     6481 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     6541 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     6601 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     6661 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     6721 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     6781 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     6841 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     6901 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     6961 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     7021 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     7081 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     7141 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     7201 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     7261 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     7321 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     7381 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     7441 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     7501 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     7561 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     7621 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     7681 aaaagtgcca cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     7741 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     7801 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     7861 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     7921 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     7981 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     8041 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     8101 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt ccattcgcca
     8161 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
     8221 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
     8281 tcacgacgtt gtaaaacgac ggccagtgaa ttgtaatacg actcactata gggcgaattg
     8341 ggtacgtacc gggcccctag tatgtatgta agttaataaa acccattttt gcggaaagta
     8401 gataaaaaaa acattttttt tttttactgc actggatatc attgaactta tctgatcagt
     8461 tttaaattta cttcgatcca agggtatttg atgtaccagg ttctttcgat tacctctcac
     8521 tcaaaatgac attccactca aagtcagcgc tgtttgcctc cttctctgtc cacagaaata
     8581 tcgccgtctc tttcgccgct gcgtccgcta tctctttcgc caccgtttgt agcgttacgt
     8641 agcgtcaatg tccgccttca gttgcatttt gtcagcggtt tcgtgacgaa gctccaagcg
     8701 gtttacgcca tcaattaaac acaaagtgct gtgccaaaac tcctctcgct tcttattttt
     8761 gtttgttttt tgagtgattg gggtggtgat tggttttggg tgggtaagca ggggaaagtg
     8821 tgaaaaatcc cggcaatggg ccaagaggat caggagctat taattcgcgg aggcagcaaa
     8881 cacccatctg ccgagcatct gaacaatgtg agtagtacat gtgcatacat cttaagttca
     8941 cttgatctat aggaactgcg attgcaacat caaattgtct gcggcgtgag aactgcgacc
     9001 cacaaaaatc ccaaaccgca attgcacaaa caaatagtga cacgaaacag attattctgg
     9061 tagctgttct cgctatataa gacaattttt gagatcatat catgatcaag acatctaaag
     9121 gcattcattt tcgactatat tcttttttac aaaaaatata acaaccagat attttaagct
     9181 gatcctagat gcacaaaaaa taaataaaag tataaaccta cttcgtagga tacttcgggg
     9241 tactttttgt tcggggttag atgagcataa cgcttgtagt tgatatttga gatcccctat
     9301 cattgcaggg tgacagcgga gcggcttcgc agagctgcat taaccagggc ttcgggcagg
     9361 ccaaaaacta cggcacgctc cggccaccca gtccgccgga ggactccggt tcagggagcg
     9421 gccaactagc cgagaacctc acctatgcct ggcacaatat ggacatcttt ggggcggtca
     9481 atcagccggg ctccggatgg cggcagctgg tcaaccggac acgcggacta ttctgcaacg
     9541 agcgacacat accggcgccc aggaaacatt tgctcaagaa cggtgagttt ctattcgcag
     9601 tcggctgatc tgtgtgaaat cttaataaag ggtccaatta ccaatttgaa actcagtttg
     9661 cggcgtggcc tatccgggcg aacttttggc cgtgatgggc agttccggtg ccggaaagac
     9721 gaccctgctg aatgcccttg cctttcgatc gccgcagggc atccaagtat cgccatccgg
     9781 gatgcgactg ctcaatggcc aacctgtgga cgccaaggag atgcaggcca ggtgcgccta
     9841 tgtccagcag gatgacctct ttatcggctc cctaacggcc agggaacacc tgattttcca
     9901 ggccatggtg cggatgccac gacatctgac ctatcggcag cgagtggccc gcgtggatca
     9961 ggtgatccag gagctttcgc tcagcaaatg tcagcacacg atcatcggtg tgcccggcag
    10021 ggtgaaaggt ctgtccggcg gagaaaggaa gcgtctggca ttcgcctccg aggcactaac
    10081 cgatccgccg cttctgatct gcgatgagcc cacctccgga ctggactcat ttaccgccca
    10141 cagcgtcgtc caggtgctga agaagctgtc gcagaagggc aagaccgtca tcctgaccat
    10201 tcatcagccg tcttccgagc tgtttgagct ctttgacaag atccttctga tggccgaggg
    10261 cagggtagct ttcttgggca ctcccagcga agccgtcgac ttcttttcct agtgagttcg
    10321 atgtgtttat taagggtatc tagcattaca ttacatctca actcctatcc agcgtgggtg
    10381 cccagtgtcc taccaactac aatccggcgg acttttacgt acaggtgttg gccgttgtgc
    10441 ccggacggga gatcgagtcc cgtgatcgga tcgccaagat atgcgacaat tttgctatta
    10501 gcaaagtagc ccgggatatg gagcagttgt tggccaccaa aaatttggag aagccactgg
    10561 agcagccgga gaatgggtac acctacaagg ccacctggtt catgcagttc cgggcggtcc
    10621 tgtggcgatc ctggctgtcg gtgctcaagg aaccactcct cgtaaaagtg cgacttattc
    10681 agacaacggt gagtggttcc agtggaaaca aatgatataa cgcttacaat tcttggaaac
    10741 aaattcgcta gattttagtt agaattgcct gattccacac ccttcttagt ttttttcaat
    10801 gagatgtata gtttatagtt ttgcagaaaa taaataaatt tcatttaact cgcgaacatg
    10861 ttgaagatat gaatattaat gagatgcgag taacatttta atttgcagat ggttgccatc
    10921 ttgattggcc tcatcttttt gggccaacaa ctcacgcaag tgggcgtgat gaatatcaac
    10981 ggagccatct tcctcttcct gaccaacatg acctttcaaa acgtctttgc cacgataaat
    11041 gtaagtcttg tttagaatac atttgcatat taataattta ctaactttct aatgaatcga
    11101 ttcgatttag gtgttcacct cagagctgcc agtttttatg agggaggccc gaagtcgact
    11161 ttatcgctgt gacacatact ttctgggcaa aacgattgcc gaattaccgc tttttctcac
    11221 agtgccactg gtcttcacgg cgattgccta tccgatgatc ggactgcggg ccggagtgct
    11281 gcacttcttc aactgcctgg cgctggtcac tctggtggcc aatgtgtcaa cgtccttcgg
    11341 atatctaata tcctgcgcca gctcctcgac ctcgatggcg ctgtctgtgg gtccgccggt
    11401 tatcatacca ttcctgctct ttggcggctt cttcttgaac tcgggctcgg tgccagtata
    11461 cctcaaatgg ttgtcgtacc tctcatggtt ccgttacgcc aacgagggtc tgctgattaa
    11521 ccaatgggcg gacgtggagc cgggcgaaat tagctgcaca tcgtcgaaca ccacgtgccc
    11581 cagttcgggc aaggtcatcc tggagacgct taacttctcc gccgccgatc tgccgctgga
    11641 ctacgtgggt ctggccattc tcatcgtgag cttccgggtg ctcgcatatc tggctctaag
    11701 acttcgggcc cgacgcaagg agtagccgac atatatccga aataactgct tgtttttttt
    11761 ttttaccatt attaccatcg tgtttactgt ttattgcccc ctcaaaaagc taatgtaatt
    11821 atatttgtgc caataaaaac aagatatgac ctatagaata caagtatttc cccttcgaac
    11881 atccccacaa gtagactttg gatttgtctt ctaaccaaaa gacttacaca cctgcatacc
    11941 ttacatcaaa aactcgttta tcgctacata aaacaccggg atatattttt tatatacata
    12001 cttttcaaat cgcgcgccct cttcataatt cacctccacc acaccacgtt tcgtagttgc
    12061 tctttcgctg tctcccaccc gctctccgca acacattcac cttttgttcg acgaccttgg
    12121 agcgactgtc gttagttccg cgcgattcgg ttcgctcaaa tggttccgag tggttcattt
    12181 cgtctcaata gaaattagta ataaatattt gtatgtacaa tttatttgct ccaatatatt
    12241 tgtatatatt tccctcacag ctatatttat tctaatttaa tattatgact ttttaaggta
    12301 attttttgtg acctgttcgg agtgattagc gttacaattt gaactgaaag tgacatccag
    12361 tgtttgttcc ttgtgtagat gcatctcaaa aaaatggtgg gcataatagt gttgtttata
    12421 tatatcaaaa ataacaacta taataataag aatacattta atttagaaaa tgcttggatt
    12481 tcactggaac tag
//
