LOCUS       sequence-131066-       10648 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Generating transgenic flies for FLP-inducible DamID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10648)
  AUTHORS   Pindyurin AV, Pagie L, Kozhevnikova EN, van Arensbergen J, van 
            Steensel B
  TITLE     Inducible DamID systems for genomic mapping of chromatin proteins in
            Drosophila.
  JOURNAL   Nucleic Acids Res. 2016 Jul 8;44(12):5646-57. doi: 
            10.1093/nar/gkw176. Epub 2016 Mar 21.
  PUBMED    27001518
REFERENCE   2  (bases 1 to 10648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkw176"; journalName: "Nucleic Acids Res"; date: 
            "2016-07-8- 8"; volume: "44"; issue: "12"; pages: "5646-57"
COMMENT     Sequence Label: p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMyc[closed]
FEATURES             Location/Qualifiers
     source          1..10648
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    7..40
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        46..284
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     231..252
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     protein_bind    304..351
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     intron          867..932
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1062..1082
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1507..1641
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    1669..1716
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             2566..2595
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     intron          2720..2785
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2915..2935
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3207..3341
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    3370..3439
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(3658..3676)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3697..3713)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3697..3713)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3710..3732)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3721..3737
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3745..3751,3752..3769,3770..3775))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3790..3811
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3928..3945)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4099..4687)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4179..4198)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4858..5649,5650..5718))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5481..5500
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5719..5823)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5850..6305
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5937..5956)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6147..6168
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6431..6453
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6445..6462
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6446..6462
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6469..6488
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6469..6487
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gene            6512..10648
                     /gene="white"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
     primer_bind     complement(6521..6539)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(6991..7062,7464..7737,7812..8466,8528..8843,9064..9195,
                     9266..9880)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
ORIGIN
        1 gctagcataa cttcgtataa tgtatgctat acgaagttat gagcgccgga gtataaatag
       61 aggcgcttcg tctacggagc gacaattcaa ttcaaacaag caaagtgaac acgtcgctaa
      121 gcgaaagcta agcaaataaa caagcgcagc tgaacaagct aaacaatctg cagtaaagtg
      181 caagttaaag tgaatcaatt aaaagtaacc agcaaccaag taaatcaact gcaactactg
      241 aaatctgcca agaagtaatt attgaataca agaagagaac tctgaatagg gaattgggaa
      301 ttcgaagttc ctattccgaa gttcctattc tctagaaagt ataggaactt ctcggggaca
      361 ccaaatatgg cgatctcggc cttttcgttt cttggagctg ggacatgttt gccatcgatc
      421 catctaccac cagaacggcc gttagatctg ctgccaccgt tgtttccacc gaagaaacca
      481 ccgttgccgt aaccaccacg acggttgttg ctaaagaagc tgccaccgcc acggccaccg
      541 ttgtagccgc cgttgttgtt attgtagttg ctactgttat ttctggcact tcttggtttt
      601 cctcttaagt gaggaggaac ataaccattc tcgttgttgt cgttgatgct taaattttgc
      661 acttgttcgc tcagttcagc cataatatga aatgcttttc ttgttgttct tacggaatac
      721 cacttgccac ctatcaccac aactaacttt ttcccgttcc tccatctctt ttatattttt
      781 tttctcgagg gatctttgtg aaggaacctt acttctgtgg tgtgacataa ttggacaaac
      841 tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta taatgtgtta
      901 aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact gatgaatggg
      961 agcagtggtg gaatgccttt aatgaggaaa acctgttttg ctcagaagaa atgccatcta
     1021 gtgatgatga ggctactgct gactctcaac attctactcc tccaaaaaag aagagaaagg
     1081 tagaagaccc caaggacttt ccttcagaat tgctaagttt tttgagtcat gctgtgttta
     1141 gtaatagaac tcttgcttgc tttgctattt acaccacaaa ggaaaaagct gcactgctat
     1201 acaagaaaat tatggaaaaa tattctgtaa cctttataag taggcataac agttataatc
     1261 ataacatact gttttttctt actccacaca ggcatagagt gtctgctatt aataactatg
     1321 ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg ggttaataag gaatatttga
     1381 tgtatagtgc cttgactaga gatcataatc agccatacca catttgtaga ggttttactt
     1441 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     1501 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     1561 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     1621 gtatcttatc atgtctggat ctgacatggt aagtaagctt gggctgcaga agttcctatt
     1681 ccgaagttcc tattctctag aaagtatagg aacttcgaat tcaacatgaa gaaaaatcgc
     1741 gcttttttga agtgggcagg gggcaagtat cccctgcttg atgatattaa acggcatttg
     1801 cccaagggcg aatgtctggt tgagcctttt gtaggtgccg ggtcggtgtt tctcaacacc
     1861 gacttttctc gttatatcct tgccgatatc aatagcgacc tgatcagtct ctataacatt
     1921 gtgaagatgc gtactgatga gtacgtacag gccgcacgcg agctgtttgt tcccgaaaca
     1981 aattgcgccg aggtttacta tcagttccgc gaagagttca acaaaagcca ggatccgttc
     2041 cgtcgggcgg tactgttttt atatttgaac cgctacggtt acaacggcct gtgtcgttac
     2101 aatctgcgcg gtgagtttaa cgtgccgttc ggccgctaca aaaaacccta tttcccggaa
     2161 gcagagttgt atcacttcgc tgaaaaagcg cagaatgcct ttttctattg tgagtcttac
     2221 gccgatagca tggcgcgcgc agatgatgca tccgtcgtct attgcgatcc gccttatgca
     2281 ccgctgtctg cgaccgccaa ctttacggcg tatcacacaa acagttttac gcttgaacaa
     2341 caagcgcatc tggcggagat cgccgaaggt ctggttgagc gccatattcc agtgctgatc
     2401 tccaatcacg atacgatgtt aacgcgtgag tggtatcagc gcgcaaaatt gcatgtcgtc
     2461 aaagttcgac gcagtataag cagcaacggc ggcacacgta aaaaggtgga cgaactgctg
     2521 gctttgtaca aaccaggagt cgtttcaccc gcgaaaaaag ccggtgaaca gaaactcatc
     2581 tctgaagagg atctgtaagg cgcgccattt aaatggccgg ccgcgatcgc ggccgctcta
     2641 gaggatcttt gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac
     2701 agagatttaa agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact
     2761 gattctaatt gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg
     2821 gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga
     2881 tgaggctact gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga
     2941 ccccaaggac tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag
     3001 aactcttgct tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa
     3061 aattatggaa aaatatttga tgtatagtgc cttgactaga gatcataatc agccatacca
     3121 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     3181 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     3241 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     3301 gtttgtccaa actcatcaat gtatcttatc atgtctggat ccactagtgt cgacgatgta
     3361 ggtcacggtc tcgaagccgc ggtgcgggtg ccagggcgtg cccttgggct ccccgggcgc
     3421 gtactccacc tcacccatct ggtccatcat gatgaacggg tcgaggtggc ggtagttgat
     3481 cccggcgaac gcgcggcgca ccgggaagcc ctcgccctcg aaaccgctgg gcgcggtggt
     3541 cacggtgagc acgggacgtg cgacggcgtc ggcgggtgcg gatacgcggg gcagcgtcag
     3601 cgggttctcg acggtcacgg cgggcatgtc gacactagtt ctagccagct tttgttccct
     3661 ttagtgaggg ttaatttcga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     3721 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     3781 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     3841 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     3901 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     3961 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     4021 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     4081 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     4141 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     4201 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     4261 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     4321 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     4381 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     4441 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     4501 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     4561 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     4621 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     4681 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     4741 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     4801 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     4861 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     4921 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     4981 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     5041 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     5101 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     5161 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     5221 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     5281 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     5341 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     5401 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     5461 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     5521 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     5581 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     5641 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     5701 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     5761 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     5821 gcgcacattt ccccgaaaag tgccacctga cgcgccctgt agcggcgcat taagcgcggc
     5881 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     5941 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     6001 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     6061 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     6121 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     6181 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     6241 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     6301 aatttccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
     6361 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
     6421 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgaattgta atacgactca
     6481 ctatagggcg aattgggtac gtaccgggcc cctagtatgt atgtaagtta ataaaaccca
     6541 tttttgcgga aagtagataa aaaaaacatt tttttttttt actgcactgg atatcattga
     6601 acttatctga tcagttttaa atttacttcg atccaagggt atttgatgta ccaggttctt
     6661 tcgattacct ctcactcaaa atgacattcc actcaaagtc agcgctgttt gcctccttct
     6721 ctgtccacag aaatatcgcc gtctctttcg ccgctgcgtc cgctatctct ttcgccaccg
     6781 tttgtagcgt tacgtagcgt caatgtccgc cttcagttgc attttgtcag cggtttcgtg
     6841 acgaagctcc aagcggttta cgccatcaat taaacacaaa gtgctgtgcc aaaactcctc
     6901 tcgcttctta tttttgtttg ttttttgagt gattggggtg gtgattggtt ttgggtgggt
     6961 aagcagggga aagtgtgaaa aatcccggca atgggccaag aggatcagga gctattaatt
     7021 cgcggaggca gcaaacaccc atctgccgag catctgaaca atgtgagtag tacatgtgca
     7081 tacatcttaa gttcacttga tctataggaa ctgcgattgc aacatcaaat tgtctgcggc
     7141 gtgagaactg cgacccacaa aaatcccaaa ccgcaattgc acaaacaaat agtgacacga
     7201 aacagattat tctggtagct gttctcgcta tataagacaa tttttgagat catatcatga
     7261 tcaagacatc taaaggcatt cattttcgac tatattcttt tttacaaaaa atataacaac
     7321 cagatatttt aagctgatcc tagatgcaca aaaaataaat aaaagtataa acctacttcg
     7381 taggatactt cggggtactt tttgttcggg gttagatgag cataacgctt gtagttgata
     7441 tttgagatcc cctatcattg cagggtgaca gcggagcggc ttcgcagagc tgcattaacc
     7501 agggcttcgg gcaggccaaa aactacggca cgctccggcc acccagtccg ccggaggact
     7561 ccggttcagg gagcggccaa ctagccgaga acctcaccta tgcctggcac aatatggaca
     7621 tctttggggc ggtcaatcag ccgggctccg gatggcggca gctggtcaac cggacacgcg
     7681 gactattctg caacgagcga cacataccgg cgcccaggaa acatttgctc aagaacggtg
     7741 agtttctatt cgcagtcggc tgatctgtgt gaaatcttaa taaagggtcc aattaccaat
     7801 ttgaaactca gtttgcggcg tggcctatcc gggcgaactt ttggccgtga tgggcagttc
     7861 cggtgccgga aagacgaccc tgctgaatgc ccttgccttt cgatcgccgc agggcatcca
     7921 agtatcgcca tccgggatgc gactgctcaa tggccaacct gtggacgcca aggagatgca
     7981 ggccaggtgc gcctatgtcc agcaggatga cctctttatc ggctccctaa cggccaggga
     8041 acacctgatt ttccaggcca tggtgcggat gccacgacat ctgacctatc ggcagcgagt
     8101 ggcccgcgtg gatcaggtga tccaggagct ttcgctcagc aaatgtcagc acacgatcat
     8161 cggtgtgccc ggcagggtga aaggtctgtc cggcggagaa aggaagcgtc tggcattcgc
     8221 ctccgaggca ctaaccgatc cgccgcttct gatctgcgat gagcccacct ccggactgga
     8281 ctcatttacc gcccacagcg tcgtccaggt gctgaagaag ctgtcgcaga agggcaagac
     8341 cgtcatcctg accattcatc agccgtcttc cgagctgttt gagctctttg acaagatcct
     8401 tctgatggcc gagggcaggg tagctttctt gggcactccc agcgaagccg tcgacttctt
     8461 ttcctagtga gttcgatgtg tttattaagg gtatctagca ttacattaca tctcaactcc
     8521 tatccagcgt gggtgcccag tgtcctacca actacaatcc ggcggacttt tacgtacagg
     8581 tgttggccgt tgtgcccgga cgggagatcg agtcccgtga tcggatcgcc aagatatgcg
     8641 acaattttgc tattagcaaa gtagcccggg atatggagca gttgttggcc accaaaaatt
     8701 tggagaagcc actggagcag ccggagaatg ggtacaccta caaggccacc tggttcatgc
     8761 agttccgggc ggtcctgtgg cgatcctggc tgtcggtgct caaggaacca ctcctcgtaa
     8821 aagtgcgact tattcagaca acggtgagtg gttccagtgg aaacaaatga tataacgctt
     8881 acaattcttg gaaacaaatt cgctagattt tagttagaat tgcctgattc cacacccttc
     8941 ttagtttttt tcaatgagat gtatagttta tagttttgca gaaaataaat aaatttcatt
     9001 taactcgcga acatgttgaa gatatgaata ttaatgagat gcgagtaaca ttttaatttg
     9061 cagatggttg ccatcttgat tggcctcatc tttttgggcc aacaactcac gcaagtgggc
     9121 gtgatgaata tcaacggagc catcttcctc ttcctgacca acatgacctt tcaaaacgtc
     9181 tttgccacga taaatgtaag tcttgtttag aatacatttg catattaata atttactaac
     9241 tttctaatga atcgattcga tttaggtgtt cacctcagag ctgccagttt ttatgaggga
     9301 ggcccgaagt cgactttatc gctgtgacac atactttctg ggcaaaacga ttgccgaatt
     9361 accgcttttt ctcacagtgc cactggtctt cacggcgatt gcctatccga tgatcggact
     9421 gcgggccgga gtgctgcact tcttcaactg cctggcgctg gtcactctgg tggccaatgt
     9481 gtcaacgtcc ttcggatatc taatatcctg cgccagctcc tcgacctcga tggcgctgtc
     9541 tgtgggtccg ccggttatca taccattcct gctctttggc ggcttcttct tgaactcggg
     9601 ctcggtgcca gtatacctca aatggttgtc gtacctctca tggttccgtt acgccaacga
     9661 gggtctgctg attaaccaat gggcggacgt ggagccgggc gaaattagct gcacatcgtc
     9721 gaacaccacg tgccccagtt cgggcaaggt catcctggag acgcttaact tctccgccgc
     9781 cgatctgccg ctggactacg tgggtctggc cattctcatc gtgagcttcc gggtgctcgc
     9841 atatctggct ctaagacttc gggcccgacg caaggagtag ccgacatata tccgaaataa
     9901 ctgcttgttt ttttttttta ccattattac catcgtgttt actgtttatt gccccctcaa
     9961 aaagctaatg taattatatt tgtgccaata aaaacaagat atgacctata gaatacaagt
    10021 atttcccctt cgaacatccc cacaagtaga ctttggattt gtcttctaac caaaagactt
    10081 acacacctgc ataccttaca tcaaaaactc gtttatcgct acataaaaca ccgggatata
    10141 ttttttatat acatactttt caaatcgcgc gccctcttca taattcacct ccaccacacc
    10201 acgtttcgta gttgctcttt cgctgtctcc cacccgctct ccgcaacaca ttcacctttt
    10261 gttcgacgac cttggagcga ctgtcgttag ttccgcgcga ttcggttcgc tcaaatggtt
    10321 ccgagtggtt catttcgtct caatagaaat tagtaataaa tatttgtatg tacaatttat
    10381 ttgctccaat atatttgtat atatttccct cacagctata tttattctaa tttaatatta
    10441 tgacttttta aggtaatttt ttgtgacctg ttcggagtga ttagcgttac aatttgaact
    10501 gaaagtgaca tccagtgttt gttccttgtg tagatgcatc tcaaaaaaat ggtgggcata
    10561 atagtgttgt ttatatatat caaaaataac aactataata ataagaatac atttaattta
    10621 gaaaatgctt ggatttcact ggaactag
//
