LOCUS       sequence-131070-       11824 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Generating transgenic flies for 4-hydroxytamoxifen-inducible DamID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11824)
  AUTHORS   Pindyurin AV, Pagie L, Kozhevnikova EN, van Arensbergen J, van 
            Steensel B
  TITLE     Inducible DamID systems for genomic mapping of chromatin proteins in
            Drosophila.
  JOURNAL   Nucleic Acids Res. 2016 Jul 8;44(12):5646-57. doi: 
            10.1093/nar/gkw176. Epub 2016 Mar 21.
  PUBMED    27001518
REFERENCE   2  (bases 1 to 11824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkw176"; journalName: "Nucleic Acids Res"; date: 
            "2016-07-8- 8"; volume: "44"; issue: "12"; pages: "5646-57"
COMMENT     Sequence Label: p-attB-FL.hsp70P-Dam[4-HT-intein@L127C]MycPC
FEATURES             Location/Qualifiers
     source          1..11824
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    7..40
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        42..494
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     441..462
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             2593..2622
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3031..3060
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             3109..3132
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     intron          3896..3961
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4091..4111
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4383..4517
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4420..4439)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4474..4493
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    4546..4615
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(4834..4852)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4873..4889)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4873..4889)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4886..4908)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4897..4913
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4921..4927,4928..4945,4946..4951))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4966..4987
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5104..5121)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5275..5863)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5355..5374)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6034..6825,6826..6894))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6657..6676
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6895..6999)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7026..7481
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7113..7132)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7323..7344
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7607..7629
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7621..7638
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7622..7638
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7645..7664
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7645..7663
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gene            7688..11824
                     /gene="white"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
     primer_bind     complement(7697..7715)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(8167..8238,8640..8913,8988..9642,9704..10019,
                     10240..10371,10442..11056)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
ORIGIN
        1 gctagcataa cttcgtataa tgtatgctat acgaagttat cctagaatcc caaaacaaac
       61 tggttattgt ggtaggtcat ttgtttggca gaaagaaaac tcgagaaatt tctctggccg
      121 ttattcgtta ttctctcttt tctttttggg tctctccctc tctgcactaa tgctctctca
      181 ctctgtcaca cagtaaacgg catactgctc tcgttggttc gagagagcgc gcctcgaatg
      241 ttcgcgaaaa gagcgccgga gtataaatag aggcgcttcg tctacggagc gacaattcaa
      301 ttcaaacaag caaagtgaac acgtcgctaa gcgaaagcta agcaaataaa caagcgcagc
      361 tgaacaagct aaacaatctg cagtaaagtg caagttaaag tgaatcaatt aaaagtaacc
      421 agcaaccaag taaatcaact gcaactactg aaatctgcca agaagtaatt attgaataca
      481 agaagagaac tctgaatagg gaattgggaa ttcaacatga agaaaaatcg cgcttttttg
      541 aagtgggcag ggggcaagta tcccctgctt gatgatatta aacggcattt gcccaagggc
      601 gaatgtctgg ttgagccttt tgtaggtgcc gggtcggtgt ttctcaacac cgacttttct
      661 cgttatatcc ttgccgatat caatagcgac ctgatcagtc tctataacat tgtgaagatg
      721 cgtactgatg agtacgtaca ggccgcacgc gagctgtttg ttcccgaaac aaattgcgcc
      781 gaggtttact atcagttccg cgaagagttc aacaaaagcc aggatccgtt ccgtcgggcg
      841 gtactgtttt tatatttgaa ccgctacggt tacaacggcc tgtgtcgtta caattgcctt
      901 gccgagggta cccgaatctt cgatccggtc actggtacaa cgcatcgcat cgaggatgtt
      961 gtcgatgggc gcaagcctat tcatgtcgtg gctgttgcca aggacggaac gctgctcgcg
     1021 cggcccgtgg tgtcctggtt cgaccaggga acgcgggatg tgatcgggtt gcggatcgcc
     1081 ggtggcgcca tcgtgtgggc gacacccgat cacaaggtgc tgacagagta cggctggcgt
     1141 gccgccgggg aactccgcaa gggagacagg gtggccggac cgggtggttc tggtaacagc
     1201 ctggccttgt ccctgacggc cgaccagatg gtcagtgcct tgttggatgc tgagcccccc
     1261 atactctatt ccgagtatga tcctaccagt cccttcagtg aagcttcgat gatgggctta
     1321 ctgaccaacc tggcagacag ggagctggtt cacatgatca actgggcgaa gagggtgcca
     1381 ggctttgtgg atttgaccct ccatgatcag gcccaccttc tagaatgtgc ctggctagag
     1441 atcctgatga ttggtctcgt ctggcgctcc atggagcacc cagggaagct actgtttgct
     1501 cctaacttgc tcttggacag gaaccaggga aaatgtgtag agggcatggt ggagatcttc
     1561 gacatgctgc tggctacatc atctcggttc cgcatgatga atctgcaggg agaggagttt
     1621 gtgtgcctca aatctattat tttgcttaat tctggagtgt acacatttct gtccagcacc
     1681 ctgaagtctc tggaagagaa ggaccatatc caccgagccc tggacaagat cacggacact
     1741 ttgatccacc tgatggccaa ggcaggcctg accctgcagc agcagcacca gcggctggcc
     1801 cagctcctcc tcatcctctc ccacatcagg cacatgagta acaaaggaat ggagcatctg
     1861 tacagcatga agtacacgaa cgtggtgccc ctctatgacc tgctgctgga gatgctggac
     1921 gcccaccgcc tacatgcggg tggttctggt gctagccgcg tgcaggcgtt cgcggatgcc
     1981 ctggatgaca aattcctgca cgacatgctg gcggaagaac tccgctattc cgtgatccga
     2041 gaagtgctgc caacgcggcg ggcacgaacg ttcgacctcg aggtcgagga actgcacacc
     2101 ctcgtcgccg aaggggttgt cgtgcacaac tgtcgcggtg agtttaacgt gccgttcggc
     2161 cgctacaaaa aaccctattt cccggaagca gagttgtatc acttcgctga aaaagcgcag
     2221 aatgcctttt tctattgtga gtcttacgcc gatagcatgg cgcgcgcaga tgatgcatcc
     2281 gtcgtctatt gcgatccgcc ttatgcaccg ctgtctgcga ccgccaactt tacggcgtat
     2341 cacacaaaca gttttacgct tgaacaacaa gcgcatctgg cggagatcgc cgaaggtctg
     2401 gttgagcgcc atattccagt gctgatctcc aatcacgata cgatgttaac gcgtgagtgg
     2461 tatcagcgcg caaaattgca tgtcgtcaaa gttcgacgca gtataagcag caacggcggc
     2521 acacgtaaaa aggtggacga actgctggct ttgtacaaac caggagtcgt ttcacccgcg
     2581 aaaaaagccg gtgaacagaa actcatctct gaagaggatc tgggcgcgcc tatgactggt
     2641 cgaggcaagg ggagtaaggg gaagttgggg cgcgacaatg cgaccgacga tccagtcgat
     2701 ctagtgtacg cggctgagaa aatcatccaa aagcgcgtta agaagggcgt cgtggagtac
     2761 cgtgtcaagt ggaagggctg gaaccagcgc tacaacacct gggaaccgga ggtaaacatc
     2821 ctggatcgcc gcctcatcga catctacgaa caaacgaaca aatcctccgg aactccctcc
     2881 aagcgaggca ttaagaagaa ggagaaagaa cccgatccgg agccggaatc cgaggaggat
     2941 gagtacacct tcaccgaaaa cgatgtggac acgcatcaag ccaccacctc atcggctacc
     3001 cacgataagg agtcgaagaa ggagaagaag caccatcacc accaccacca tcatcaccac
     3061 atcaagtccg aacgcaacag tggacggcgc tcggaatctc cgctgaccca ccatcatcat
     3121 caccaccacc acgagtccaa gcgtcagcgc attgatcaca gctcctcctc gaacagcagc
     3181 ttcacgcaca actcatttgt ccccgagccg gacagcaact cctccagctc cgaggatcag
     3241 ccgctgatcg gcaccaaacg caaagccgag gtgctcaagg aatcgggcaa gattggagtt
     3301 acgattaaga cctccccaga tggccccact attaagcctc agccgaccca gcaggtaact
     3361 cccagccagc agcagccctt ccaggatcag cagcaagcgg aaaagattgc cagcgaggct
     3421 gcaacgcagc tgaaatctga gcagcaggcc accccactgg ccacagaagc cataaacaca
     3481 acgcccgcag agtccggagc tgaggaagaa gaagtagcca acgaggaagg caaccaacag
     3541 gcgccacagg ttccttccga gaacaataac ataccaaaac cgtgcaacaa cctggctatc
     3601 aaccagaaac agccgcttac tcctctttcg ccgcgtgccc ttccgccgcg cttctggctg
     3661 ccggccaaat gcaacatatc caaccgggtg gtgatcacag acgtcaccgt aaacctggag
     3721 accgttacca tacgcgagtg caagacggag cgcgggtttt ttcgtgagcg cgacatgaaa
     3781 ggcgattcgt cgccagtagc ttgagcggcc gctctagagg atctttgtga aggaacctta
     3841 cttctgtggt gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa
     3901 tataaaattt ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta
     3961 gattccaacc tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa
     4021 cctgttttgc tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca
     4081 ttctactcct ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt
     4141 gctaagtttt ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta
     4201 caccacaaag gaaaaagctg cactgctata caagaaaatt atggaaaaat atttgatgta
     4261 tagtgccttg actagagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
     4321 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
     4381 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     4441 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     4501 cttatcatgt ctggatccac tagtgtcgac gatgtaggtc acggtctcga agccgcggtg
     4561 cgggtgccag ggcgtgccct tgggctcccc gggcgcgtac tccacctcac ccatctggtc
     4621 catcatgatg aacgggtcga ggtggcggta gttgatcccg gcgaacgcgc ggcgcaccgg
     4681 gaagccctcg ccctcgaaac cgctgggcgc ggtggtcacg gtgagcacgg gacgtgcgac
     4741 ggcgtcggcg ggtgcggata cgcggggcag cgtcagcggg ttctcgacgg tcacggcggg
     4801 catgtcgaca ctagttctag ccagcttttg ttccctttag tgagggttaa tttcgagctt
     4861 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     4921 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     4981 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     5041 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     5101 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     5161 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     5221 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     5281 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     5341 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     5401 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     5461 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     5521 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     5581 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     5641 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     5701 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     5761 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     5821 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     5881 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     5941 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     6001 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     6061 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     6121 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     6181 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     6241 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     6301 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     6361 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     6421 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     6481 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     6541 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     6601 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     6661 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     6721 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     6781 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     6841 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     6901 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     6961 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     7021 acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     7081 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     7141 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     7201 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     7261 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     7321 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     7381 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     7441 atttaacgcg aattttaaca aaatattaac gcttacaatt tccattcgcc attcaggctg
     7501 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa
     7561 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt
     7621 tgtaaaacga cggccagtga attgtaatac gactcactat agggcgaatt gggtacgtac
     7681 cgggccccta gtatgtatgt aagttaataa aacccatttt tgcggaaagt agataaaaaa
     7741 aacatttttt ttttttactg cactggatat cattgaactt atctgatcag ttttaaattt
     7801 acttcgatcc aagggtattt gatgtaccag gttctttcga ttacctctca ctcaaaatga
     7861 cattccactc aaagtcagcg ctgtttgcct ccttctctgt ccacagaaat atcgccgtct
     7921 ctttcgccgc tgcgtccgct atctctttcg ccaccgtttg tagcgttacg tagcgtcaat
     7981 gtccgccttc agttgcattt tgtcagcggt ttcgtgacga agctccaagc ggtttacgcc
     8041 atcaattaaa cacaaagtgc tgtgccaaaa ctcctctcgc ttcttatttt tgtttgtttt
     8101 ttgagtgatt ggggtggtga ttggttttgg gtgggtaagc aggggaaagt gtgaaaaatc
     8161 ccggcaatgg gccaagagga tcaggagcta ttaattcgcg gaggcagcaa acacccatct
     8221 gccgagcatc tgaacaatgt gagtagtaca tgtgcataca tcttaagttc acttgatcta
     8281 taggaactgc gattgcaaca tcaaattgtc tgcggcgtga gaactgcgac ccacaaaaat
     8341 cccaaaccgc aattgcacaa acaaatagtg acacgaaaca gattattctg gtagctgttc
     8401 tcgctatata agacaatttt tgagatcata tcatgatcaa gacatctaaa ggcattcatt
     8461 ttcgactata ttctttttta caaaaaatat aacaaccaga tattttaagc tgatcctaga
     8521 tgcacaaaaa ataaataaaa gtataaacct acttcgtagg atacttcggg gtactttttg
     8581 ttcggggtta gatgagcata acgcttgtag ttgatatttg agatccccta tcattgcagg
     8641 gtgacagcgg agcggcttcg cagagctgca ttaaccaggg cttcgggcag gccaaaaact
     8701 acggcacgct ccggccaccc agtccgccgg aggactccgg ttcagggagc ggccaactag
     8761 ccgagaacct cacctatgcc tggcacaata tggacatctt tggggcggtc aatcagccgg
     8821 gctccggatg gcggcagctg gtcaaccgga cacgcggact attctgcaac gagcgacaca
     8881 taccggcgcc caggaaacat ttgctcaaga acggtgagtt tctattcgca gtcggctgat
     8941 ctgtgtgaaa tcttaataaa gggtccaatt accaatttga aactcagttt gcggcgtggc
     9001 ctatccgggc gaacttttgg ccgtgatggg cagttccggt gccggaaaga cgaccctgct
     9061 gaatgccctt gcctttcgat cgccgcaggg catccaagta tcgccatccg ggatgcgact
     9121 gctcaatggc caacctgtgg acgccaagga gatgcaggcc aggtgcgcct atgtccagca
     9181 ggatgacctc tttatcggct ccctaacggc cagggaacac ctgattttcc aggccatggt
     9241 gcggatgcca cgacatctga cctatcggca gcgagtggcc cgcgtggatc aggtgatcca
     9301 ggagctttcg ctcagcaaat gtcagcacac gatcatcggt gtgcccggca gggtgaaagg
     9361 tctgtccggc ggagaaagga agcgtctggc attcgcctcc gaggcactaa ccgatccgcc
     9421 gcttctgatc tgcgatgagc ccacctccgg actggactca tttaccgccc acagcgtcgt
     9481 ccaggtgctg aagaagctgt cgcagaaggg caagaccgtc atcctgacca ttcatcagcc
     9541 gtcttccgag ctgtttgagc tctttgacaa gatccttctg atggccgagg gcagggtagc
     9601 tttcttgggc actcccagcg aagccgtcga cttcttttcc tagtgagttc gatgtgttta
     9661 ttaagggtat ctagcattac attacatctc aactcctatc cagcgtgggt gcccagtgtc
     9721 ctaccaacta caatccggcg gacttttacg tacaggtgtt ggccgttgtg cccggacggg
     9781 agatcgagtc ccgtgatcgg atcgccaaga tatgcgacaa ttttgctatt agcaaagtag
     9841 cccgggatat ggagcagttg ttggccacca aaaatttgga gaagccactg gagcagccgg
     9901 agaatgggta cacctacaag gccacctggt tcatgcagtt ccgggcggtc ctgtggcgat
     9961 cctggctgtc ggtgctcaag gaaccactcc tcgtaaaagt gcgacttatt cagacaacgg
    10021 tgagtggttc cagtggaaac aaatgatata acgcttacaa ttcttggaaa caaattcgct
    10081 agattttagt tagaattgcc tgattccaca cccttcttag tttttttcaa tgagatgtat
    10141 agtttatagt tttgcagaaa ataaataaat ttcatttaac tcgcgaacat gttgaagata
    10201 tgaatattaa tgagatgcga gtaacatttt aatttgcaga tggttgccat cttgattggc
    10261 ctcatctttt tgggccaaca actcacgcaa gtgggcgtga tgaatatcaa cggagccatc
    10321 ttcctcttcc tgaccaacat gacctttcaa aacgtctttg ccacgataaa tgtaagtctt
    10381 gtttagaata catttgcata ttaataattt actaactttc taatgaatcg attcgattta
    10441 ggtgttcacc tcagagctgc cagtttttat gagggaggcc cgaagtcgac tttatcgctg
    10501 tgacacatac tttctgggca aaacgattgc cgaattaccg ctttttctca cagtgccact
    10561 ggtcttcacg gcgattgcct atccgatgat cggactgcgg gccggagtgc tgcacttctt
    10621 caactgcctg gcgctggtca ctctggtggc caatgtgtca acgtccttcg gatatctaat
    10681 atcctgcgcc agctcctcga cctcgatggc gctgtctgtg ggtccgccgg ttatcatacc
    10741 attcctgctc tttggcggct tcttcttgaa ctcgggctcg gtgccagtat acctcaaatg
    10801 gttgtcgtac ctctcatggt tccgttacgc caacgagggt ctgctgatta accaatgggc
    10861 ggacgtggag ccgggcgaaa ttagctgcac atcgtcgaac accacgtgcc ccagttcggg
    10921 caaggtcatc ctggagacgc ttaacttctc cgccgccgat ctgccgctgg actacgtggg
    10981 tctggccatt ctcatcgtga gcttccgggt gctcgcatat ctggctctaa gacttcgggc
    11041 ccgacgcaag gagtagccga catatatccg aaataactgc ttgttttttt tttttaccat
    11101 tattaccatc gtgtttactg tttattgccc cctcaaaaag ctaatgtaat tatatttgtg
    11161 ccaataaaaa caagatatga cctatagaat acaagtattt ccccttcgaa catccccaca
    11221 agtagacttt ggatttgtct tctaaccaaa agacttacac acctgcatac cttacatcaa
    11281 aaactcgttt atcgctacat aaaacaccgg gatatatttt ttatatacat acttttcaaa
    11341 tcgcgcgccc tcttcataat tcacctccac cacaccacgt ttcgtagttg ctctttcgct
    11401 gtctcccacc cgctctccgc aacacattca ccttttgttc gacgaccttg gagcgactgt
    11461 cgttagttcc gcgcgattcg gttcgctcaa atggttccga gtggttcatt tcgtctcaat
    11521 agaaattagt aataaatatt tgtatgtaca atttatttgc tccaatatat ttgtatatat
    11581 ttccctcaca gctatattta ttctaattta atattatgac tttttaaggt aattttttgt
    11641 gacctgttcg gagtgattag cgttacaatt tgaactgaaa gtgacatcca gtgtttgttc
    11701 cttgtgtaga tgcatctcaa aaaaatggtg ggcataatag tgttgtttat atatatcaaa
    11761 aataacaact ataataataa gaatacattt aatttagaaa atgcttggat ttcactggaa
    11821 ctag
//
