LOCUS       sequence-13108-e        3363 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1461-HQ858752
FEATURES             Location/Qualifiers
     source          1..3363
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             112..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(920..1019)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1036..1055)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1037..1055)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1060..1076)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1060..1076)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1178..1197)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1189..1998
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1256..1275)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2145..2733
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2634..2653
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2887..2904
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3063..3090)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3182..3268)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3317..3339
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3331..3348
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3332..3348
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc cttgtttaac
      121 tttaagaagg agcccttcac catgaggaag aagctcggca cccggttccc cgcggctcga
      181 atcaaaaaga taatgcaagc agatgaggat gttggaaaga ttgcactagc agttcctgtt
      241 ttagtttcga ggtctcttga attgttttta caagatttaa ttgaccgtac ttatgaaatt
      301 actcttcaaa gtggagcaaa gacactgaat tccttccacc tgaagcaatg tgtgaagagg
      361 tacagttctt ttgatttcct aactgaagtt gtcagcaagg taccagatct tggtggcgct
      421 gattcctgtg gagatgaaag aggattacct agaagaagaa agtcaaatgg cagtgaccca
      481 gagaatgatg aatcaagatc tagtaaaatg gccataagaa atgcaaacat cagccccaga
      541 ggacgtggga ggggtcgagg cagaggacga ggtcggccac caaccaagag aaaggaggtt
      601 ggttatgtac aatttgaaga tgagagcagc atgtttgctg aacaaggtga gcccttgcca
      661 ggagaggaaa cagttcaaga gatcaatggc aacgagacca tgcctcaaag cacgcaacct
      721 ccagtagagg ctccgccaac agcccttgca caagctacaa caagttntaa ggcggaagaa
      781 gcgaacagtg atcatcagtc agattggcct atgccagatg ccattggaag catcggtgtc
      841 gtgccatctg gttttggaca tctgacagtg caggttgaag atgaggacta cgacaatgag
      901 gataagggtg ggcgcgccga cccagctttc ttgtacaaag ttggcattat aagaaagcat
      961 tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgc
     1021 catccagctg atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag
     1081 ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca
     1141 tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt
     1201 caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat
     1261 aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag
     1321 cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca
     1381 gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat
     1441 tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg aaaaacagca
     1501 ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg
     1561 ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta
     1621 tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt
     1681 gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg
     1741 ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt
     1801 gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac
     1861 caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg
     1921 ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg
     1981 ctcgatgagt ttttctaatc agaattggtt aattggttgt aacactggca gagcattacg
     2041 ctgacttgac gggacggcgc aagctcatga ccaaaatccc ttaacgtgag ttacgcgtcg
     2101 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     2161 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     2221 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     2281 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     2341 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     2401 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     2461 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     2521 tacctacagc gtgagcattg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     2581 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     2641 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     2701 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     2761 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     2821 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     2881 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     2941 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     3001 ggcagtgagc gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg
     3061 caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg
     3121 ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg
     3181 gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt
     3241 cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct cgcgttaacg
     3301 ctagcatgga tgttttccca gtcacgacgt tgtaaaacga cggccagtct taagctcggg
     3361 ccc
//
