LOCUS       sequence-131080-        7138 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes for Ebola Virus Zaire VP30 under control of a T7 RNA 
            polymerase promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7138)
  AUTHORS   Trunschke M, Conrad D, Enterlein S, Olejnik J, Brauburger K, 
            Muhlberger E
  TITLE     The L-VP35 and L-L interaction domains reside in the amino terminus 
            of the Ebola virus L protein and are potential targets for 
            antivirals.
  JOURNAL   Virology. 2013 Jul 5;441(2):135-45. doi: 
            10.1016/j.virol.2013.03.013. Epub 2013 Apr 11.
  PUBMED    23582637
REFERENCE   2  (bases 1 to 7138)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.virol.2013.03.013"; journalName: "Virology"; date: 
            "2013-07-5- 5"; volume: "441"; issue: "2"; pages: "135-45"
COMMENT     Sequence Label: pTM1_VP30_ZEBOV
FEATURES             Location/Qualifiers
     source          1..7138
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     794..813
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        794..812
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    851..1423
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1018..1035)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1245..1264
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(3323..3341)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      3337..3384
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(4364..4386)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4516..4971
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4603..4622)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4813..4834
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5000..5104
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5105..5173,5174..5965)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5323..5342)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6136..6724
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6625..6644
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6878..6895
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7012..7033
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7048..7053,7054..7071,7072..7078)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7086..7102
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7091..7113
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7110..7126
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7110..7126
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttttgc gatcaataaa tggatcacaa ccagtatctc ttaacgatgt tcttcgcaga
       61 tgatgattca ttttttaagt atttggctag tcaagatgat gaatcttcat tatctgatat
      121 attgcaaatc actcaatatc tagactttct gttattatta ttgatccaat caaaaaataa
      181 attagaagcc gtgggtcatt gttatgaatc tctttcagag gaatacagac aattgacaaa
      241 attcacagac tttcaagatt ttaaaaaact gtttaacaag gtccctattg ttacagatgg
      301 aagggtcaaa cttaataaag gatatttgtt cgactttgtg attagtttga tgcgattcaa
      361 aaaagaatcc tctctagcta ccaccgcaat agatcctgtt agatacatag atcctcgtcg
      421 caatatcgca ttttctaacg tgatggatat attaaagtcg aataaagtga acaataatta
      481 attctttatt gtcatcatga acggcggaca tattcagttg ataatcggcc ccatgttttc
      541 aggtaaaagt acagaattaa ttagacgagt tagacgttat caaatagctc aatataaatg
      601 cgtgactata aaatattcta acgataatag atacggaacg ggactatgga cgcatgataa
      661 gaataatttt gaagcattgg aagcaactaa actatgtgat gtcttggaat caattacaga
      721 tttctccgtg ataggtatcg atgaaggaca gttctttcca gacattgttg aattgatctc
      781 gatcccgcga aattaatacg actcactata gggagaccac aacggtttcc ctctagcggg
      841 atcaattccg cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat
      901 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg
      961 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc
     1021 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt
     1081 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg
     1141 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac
     1201 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg
     1261 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg
     1321 ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc
     1381 cgaaccacgg ggacgtggtt ttcctttgaa aaacacgata ataccatggg aattccccgg
     1441 ggagctcact agtggatccg cgaaccggta gagtttagtt gcaacctaac acacataaag
     1501 cattggtcaa aaagtcaata gaaatttaaa cagtgagtgg agacaacttt taaatggaag
     1561 cttcatatga gagaggacgc ccacgagctg ccagacagca ttcaagggat ggacacgacc
     1621 accatgttcg agcacgatca tcatccagag agaattatcg aggtgagtac cgtcaatcaa
     1681 ggagcgcctc acaagtgcgc gttcctactg tatttcataa gaagagagtt gaaccattaa
     1741 cagttcctcc agcacctaaa gacatatgtc cgaccttgaa aaaaggattt ttgtgtgaca
     1801 gtagtttttg caaaaaagat caccagttgg agagtttaac tgatagggaa ttactcctac
     1861 taatcgcccg taagacttgt ggatcagtag aacaacaatt aaatataact gcacccaagg
     1921 actcgcgctt agcaaatcca acggctgatg atttccagca agaggaaggt ccaaaaatta
     1981 ccttgttgac actgatcaag acggcagaac actgggcgag acaagacatc agaaccatag
     2041 aggattcaaa attaagagca ttgttgactc tatgtgctgt gatgacgagg aaattctcaa
     2101 aatcccagct gagtctttta tgtgagacac acctaaggcg cgaggggctt gggcaagatc
     2161 aggcagaacc cgttctcgaa gtatatcaac gattacacag tgataaagga ggcagttttg
     2221 aagctgcact atggcaacaa tgggaccgac aatccctaat tatgtttatc actgcattct
     2281 tgaatattgc tctccagtta ccgtgtgaaa gttctgctgt cgttgtttca gggttaagaa
     2341 cattggttcc tcaatcagat aatgaggaag cttcaaccaa cccggggaca tgctcatggt
     2401 ctgatgaggg taccccttaa taaggctgac taaaacacta tataaccttc tacttgatca
     2461 caatactccg tatacctatc atcatatatt taatcaagac gatatccttt aaaacttatt
     2521 cagtactata atcactctcg tttcaaatta ataagatgtg catgattgcc ctaatatatg
     2581 aagaggtatg atacaaccct aacagtgatc aaagaaaatc ataatctcgt atcgctcgaa
     2641 atataacctg ccaagcatac ctcttgcaca aagtgattct tgtacacaaa taatgtttta
     2701 ctctacagga ggtagcaacg atccatccca tcaaaaaata agtatttcat gacttactaa
     2761 tgatctctta aaatattaag aaaaactgac ggaacataaa ttctttatgc ttcaagctgt
     2821 ggaggaggtg tttggtattg gctattgtta tattacaatc aataacaagc ttgtaaaaat
     2881 attgttcttg tttcaagagg tagattgtga ccggaaatgc taaactaatg atgaagatta
     2941 atgcggaggt ctgataagaa taaaccttat tattcagatt aggccccaag aggcattctt
     3001 catctccttt tagcaaagta ctatttcagg gtagtccaat tagtggcacg tcttttagct
     3061 gtatatcagt cgcccctgag atacgccaca aaagtgtctc taagctaaat tggtctgtac
     3121 acatcccata cattgtatta ggggcaataa tatctaattg aacttagccg tttaaaattt
     3181 agtgcataaa tctgggctaa caccaccagg tcaactccat tggctgaaaa gaagcttacc
     3241 tgcagctcga gaggcctaat taattaagtc gacgatccgg ctgctaacaa agcccgaaag
     3301 gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct
     3361 aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggatc gagatcaatt
     3421 ctgtgagcgt atggcaaacg aaggaaaaat agttatagta gccgcactcg atgggacatt
     3481 tcaacgtaaa ccgtttaata atattttgaa tcttattcca ttatctgaaa tggtggtaaa
     3541 actaactgct gtgtgtatga aatgctttaa ggaggcttcc ttttctaaac gattgggtga
     3601 ggaaaccgag atagaaataa taggaggtaa tgatatgtat caatcggtgt gtagaaagtg
     3661 ttacatcgac tcataatatt atatttttta tctaaaaaac taaaaataaa cattgattaa
     3721 attttaatat aatacttaaa aatggatgtt gtgtcgttag ataaaccgtt tatgtatttt
     3781 gaggaaattg ataatgagtt agattacgaa ccagaaagtg caaatgaggt cgcaaaaaaa
     3841 ctgccgtatc aaggacagtt aaaactatta ctaggagaat tattttttct tagtaagtta
     3901 cagcgacacg gtatattaga tggtgccacc gtagtgtata taggatctgc tcccggtaca
     3961 catatacgtt atttgagaga tcatttctat aatttaggag tgatcatcaa atggatgcta
     4021 attgacggcc gccatcatga tcctatttta aatggattgc gtgatgtgac tctagtgact
     4081 cggttcgttg atgaggaata tctacgatcc atcaaaaaac aactgcatcc ttctaagatt
     4141 attttaattt ctgatgtgag atccaaacga ggaggaaatg aacctagtac ggcggattta
     4201 ctaagtaatt acgctctaca aaatgtcatg attagtattt taaaccccgt ggcgtctagt
     4261 cttaaatgga gatgcccgtt tccagatcaa tggatcaagg acttttatat cccacacggt
     4321 aataaaatgt tacaaccttt tgctccttca tattcagggc cgtcgtttta caacgtcgtg
     4381 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     4441 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     4501 atggcgaatg gcgcgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     4561 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     4621 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     4681 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     4741 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     4801 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     4861 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     4921 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt tcccaggtgg
     4981 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     5041 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     5101 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     5161 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     5221 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     5281 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     5341 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     5401 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     5461 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     5521 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     5581 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     5641 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     5701 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     5761 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     5821 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     5881 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     5941 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     6001 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     6061 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     6121 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     6181 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     6241 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     6301 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     6361 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     6421 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     6481 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     6541 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     6601 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     6661 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     6721 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     6781 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     6841 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     6901 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     6961 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     7021 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     7081 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgcc
//
