LOCUS       sequence-13109-e        4899 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4899)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 4899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1489-HQ858753
FEATURES             Location/Qualifiers
     source          1..4899
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             112..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(2456..2555)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2572..2591)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2573..2591)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2596..2612)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2596..2612)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2714..2733)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2725..3534
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2792..2811)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3681..4269
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4170..4189
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4423..4440
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4599..4626)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4718..4804)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4853..4875
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4867..4884
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4868..4884
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc cttgtttaac
      121 tttaagaagg agcccttcac catgggcagt gcttgccaag ctggcacaga cgggccttcc
      181 cgcaaggatg tgttagggat agggaatgcc gccttagaga atggccacca tcaggctgaa
      241 gctgacgcag atgaatggag ggaaaaggaa gaggacttgg ccaacaacgg gcacagtgcg
      301 ccaccgccag gcatgcagca ggtggatgag cataaggagg aacaaagaca aagcattcac
      361 tgggagaggt tcctacctgt gaagacactg agagtcttgc tggtggagaa tgatgactct
      421 actcgtcagg tggtcagtgc cctgctccgt aagtgctgct atgaagttat tcctgctgaa
      481 aatggtttgc atgcatggcg atatcttgaa gatctgcaga acaacatcga ccttgtattg
      541 actgaggttt tcatgccttg tctatctggt atcggtctgc ttagcaaaat cacaagtcac
      601 aaaatttgca aagacattcc tgtgattatg atgtctacga atgattctat gagtatggtg
      661 tttaagtgtt tgtcgaaggg agcagttgat ttcttggtaa aaccactacg taagaatgag
      721 cttaagaacc tttggcagca tgtttggagg cgatgccaca gttccagtgg aagtgaaagt
      781 ggcatccaga cacagaagtg tgccaaacta aatactggcg acgagtatga gaacggcagt
      841 gacagcaatc atgatgatga agaaaatgat gacggcgacg atgacgactt cagtgttgga
      901 ctcaatgcta gggatggaag tgacaatggc agtggtactc aaagctcatg gacaaagcgt
      961 gctgtggaga ttgacagccc acaacctata tctcccgatc aactagttga tccacctgat
     1021 agtacatgtg cacaagtaat tcaccctaga tcagagatat gcagtaacaa gtggttaccg
     1081 acagcaaaca aaaggaatgt caagaaacag aaggagaata aagatgaatc tatgggaaga
     1141 tacttaggaa taggtgctcc taggaactca agtgcagaat atcaatcatc tctcaatgat
     1201 gtatctgtta atccaataga aaaaggacat gagaatcaca tgtccaaatg caaatctaaa
     1261 aaggaaacaa tggcagaaga tgattgtaca aacatgccta gtgcaacaaa tgctgaaact
     1321 gctgatttga ttagctcaat agccagaaac acagaaggcc aacaagcagt acaagccgtt
     1381 gacgcaccag atggcccttc caaaatggct aatggaaatg ataagaatca tgattctcat
     1441 atcgaagtga caccccatga gttgggtttg aagagatcga gaacaaatgg agctacagcg
     1501 gaaatccatg atgagcgaaa tattctgaaa agatcagatc agtcagcctt caccaggtac
     1561 catacatctg tggcttccaa tcaaggtgga gcaagatatg gggaaagctc ttcaccacaa
     1621 gataacagtt ctgaggccat gaaaacggac tctacatgca agatgaagtc aaattcagat
     1681 gctgctccaa taaagcaggg ctccaatggc agtagcaata acgatgtggg atccagtaca
     1741 aagaatgttg ctgcaaggcc ttcgggtgac agggagagag tagcgtcacc attagccatc
     1801 aaatctaccc agcatgcctc agcatttcat actatacaga atcaaacgtc accagctaat
     1861 ctgattgggg aagacaaagc tgatgaagga atttccaata cagtgaaaat gagccaccca
     1921 acagaggttc cacaaggctg cgtccagcat catcatcatg tgcattatta cctccatgtt
     1981 atgacacaga aacagccatc aacagaccgt ggatcatcag atgttcactg tggttcgtca
     2041 aatgtgtttg atcctcctgt tgaaggacat gctgcaaact acagtgtgaa tgggggtgtc
     2101 tcagttggtc ataatgggtg caatgggcag aatggaagta gcgctgtccc caatattgca
     2161 agaccaaaca tagagagtat taatggtacc atgagccaaa atattgccgg aggtggcatt
     2221 gtaagtggga gtgggagtgg caatgacatg tatcagaatc ggttcctgca acgagaagct
     2281 gcattgaaca aattcagact gaagcggaaa gatcggaact ttggtaaaaa ggttcgctac
     2341 caaagcagga agaggcttgc tgagcagcgg ccacgggtcc gaggacagtt tgtgcgacaa
     2401 tctgagcaag aagatcaaac agcgcaaggt tcagaaagaa agggtgggcg cgccgaccca
     2461 gctttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg ttgcaacgaa
     2521 caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgatat cccctatagt
     2581 gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc
     2641 tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac
     2701 ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga
     2761 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg
     2821 caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg
     2881 aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg
     2941 ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca
     3001 tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct
     3061 gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt
     3121 cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca
     3181 cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct
     3241 gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc
     3301 actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt
     3361 attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac
     3421 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat
     3481 aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa
     3541 ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga cggcgcaagc
     3601 tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta
     3661 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     3721 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     3781 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     3841 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     3901 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     3961 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     4021 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gcattgagaa
     4081 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     4141 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     4201 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     4261 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     4321 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     4381 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     4441 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     4501 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     4561 acgcgtaccg ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg
     4621 ccttctgctt agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg
     4681 gccgttgctt cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt
     4741 tcaccgacaa acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta
     4801 tttgatgcct ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca
     4861 cgacgttgta aaacgacggc cagtcttaag ctcgggccc
//
