LOCUS       sequence-131090-       12179 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes for Ebola Virus Zaire L under control of a T7 RNA polymerase
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12179)
  AUTHORS   Trunschke M, Conrad D, Enterlein S, Olejnik J, Brauburger K, 
            Muhlberger E
  TITLE     The L-VP35 and L-L interaction domains reside in the amino terminus 
            of the Ebola virus L protein and are potential targets for 
            antivirals.
  JOURNAL   Virology. 2013 Jul 5;441(2):135-45. doi: 
            10.1016/j.virol.2013.03.013. Epub 2013 Apr 11.
  PUBMED    23582637
REFERENCE   2  (bases 1 to 12179)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.virol.2013.03.013"; journalName: "Virology"; date: 
            "2013-07-5- 5"; volume: "441"; issue: "2"; pages: "135-45"
COMMENT     Sequence Label: pTM1_L_ZEBOV
FEATURES             Location/Qualifiers
     source          1..12179
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     794..813
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        794..812
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    851..1423
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1018..1035)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1245..1264
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(8364..8382)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      8378..8425
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(9405..9427)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9557..10012
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9644..9663)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9854..9875
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10041..10145
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10146..10214,10215..11006)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10364..10383)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11177..11765
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11666..11685
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11919..11936
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12053..12074
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12089..12094,12095..12112,12113..12119)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12127..12143
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12132..12154
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12151..12167
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12151..12167
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttttgc gatcaataaa tggatcacaa ccagtatctc ttaacgatgt tcttcgcaga
       61 tgatgattca ttttttaagt atttggctag tcaagatgat gaatcttcat tatctgatat
      121 attgcaaatc actcaatatc tagactttct gttattatta ttgatccaat caaaaaataa
      181 attagaagcc gtgggtcatt gttatgaatc tctttcagag gaatacagac aattgacaaa
      241 attcacagac tttcaagatt ttaaaaaact gtttaacaag gtccctattg ttacagatgg
      301 aagggtcaaa cttaataaag gatatttgtt cgactttgtg attagtttga tgcgattcaa
      361 aaaagaatcc tctctagcta ccaccgcaat agatcctgtt agatacatag atcctcgtcg
      421 caatatcgca ttttctaacg tgatggatat attaaagtcg aataaagtga acaataatta
      481 attctttatt gtcatcatga acggcggaca tattcagttg ataatcggcc ccatgttttc
      541 aggtaaaagt acagaattaa ttagacgagt tagacgttat caaatagctc aatataaatg
      601 cgtgactata aaatattcta acgataatag atacggaacg ggactatgga cgcatgataa
      661 gaataatttt gaagcattgg aagcaactaa actatgtgat gtcttggaat caattacaga
      721 tttctccgtg ataggtatcg atgaaggaca gttctttcca gacattgttg aattgatctc
      781 gatcccgcga aattaatacg actcactata gggagaccac aacggtttcc ctctagcggg
      841 atcaattccg cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat
      901 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg
      961 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc
     1021 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt
     1081 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg
     1141 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac
     1201 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg
     1261 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg
     1321 ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc
     1381 cgaaccacgg ggacgtggtt ttcctttgaa aaacacgata ataccatggg cggccgcaaa
     1441 tggctacaca acatacccaa tacccagacg ctaggttatc atcaccaatt gtattggacc
     1501 aatgtgacct agtcactaga gcttgcgggt tatattcatc atactccctt aatccgcaac
     1561 tacgcaactg taaactcccg aaacatatct accgtttgaa atacgatgta actgttacca
     1621 agttcttgag tgatgtacca gtggcgacat tgcccataga tttcatagtc ccagttcttc
     1681 tcaaggcact gtcaggcaat ggattctgtc ctgttgagcc gcggtgccaa cagttcttag
     1741 atgaaatcat taagtacaca atgcaagatg ctctcttctt gaaatattat ctcaaaaatg
     1801 tgggtgctca agaagactgt gttgatgaac actttcaaga gaaaatctta tcttcaattc
     1861 agggcaatga atttttacat caaatgtttt tctggtatga tctggctatt ttaactcgaa
     1921 ggggtagatt aaatcgagga aactctagat caacatggtt tgttcatgat gatttaatag
     1981 acatcttagg ctatggggac tatgtttttt ggaagatccc aatttcaatg ttaccactga
     2041 acacacaagg aatcccccat gctgctatgg actggtatca ggcatcagta ttcaaagaag
     2101 cggttcaagg gcatacacac attgtttctg tttctactgc cgacgtcttg ataatgtgca
     2161 aagatttaat tacatgtcga ttcaacacaa ctctaatctc aaaaatagca gagattgagg
     2221 atccagtttg ttctgattat cccaatttta agattgtgtc tatgctttac cagagcggag
     2281 attacttact ctccatatta gggtctgatg ggtataaaat tattaagttc ctcgaaccat
     2341 tgtgcttggc caaaattcaa ttatgctcaa agtacactga gaggaagggc cgattcttaa
     2401 cacaaatgca tttagctgta aatcacaccc tagaagaaat tacagaaatg cgtgcactaa
     2461 agccttcaca ggctcaaaag atccgtgaat tccatagaac attgataagg ctggagatga
     2521 cgccacaaca actttgtgag ctattttcca ttcaaaaaca ctgggggcat cctgtgctac
     2581 atagtgaaac agcaatccaa aaagttaaaa aacatgctac ggtgctaaaa gcattacgcc
     2641 ctatagtgat tttcgagaca tactgtgttt ttaaatatag tattgccaaa cattattttg
     2701 atagtcaagg atcttggtac agtgttactt cagataggaa tctaacaccg ggtcttaatt
     2761 cttatatcaa aagaaatcaa ttccctccgt tgccaatgat taaagaacta ctatgggaat
     2821 tttaccacct tgaccaccct ccacttttct caaccaaaat tattagtgac ttaagtattt
     2881 ttataaaaga cagagctacc gcagtagaaa ggacatgctg ggatgcagta ttcgagccta
     2941 atgttctagg atataatcca cctcacaaat ttagtactaa acgtgtaccg gaacaatttt
     3001 tagagcaaga aaacttttct attgagaatg ttctttccta cgcacaaaaa ctcgagtatc
     3061 tactaccaca atatcggaac ttttctttct cattgaaaga gaaagagttg aatgtaggta
     3121 gaaccttcgg aaaattgcct tatccgactc gcaatgttca aacactttgt gaagctctgt
     3181 tagctgatgg tcttgctaaa gcatttccta gcaatatgat ggtagttacg gaacgtgagc
     3241 aaaaagaaag cttattgcat caagcatcat ggcaccacac aagtgatgat tttggtgaac
     3301 atgccacagt tagagggagt agctttgtaa ctgatttaga gaaatacaat cttgcattta
     3361 gatatgagtt tacagcacct tttatagaat attgcaaccg ttgctatggt gttaagaatg
     3421 tttttaattg gatgcattat acaatcccac agtgttatat gcatgtcagt gattattata
     3481 atccaccaca taacctcaca ctggagaatc gagacaaccc ccccgaaggg cctagttcat
     3541 acaggggtca tatgggaggg attgaaggac tgcaacaaaa actctggaca agtatttcat
     3601 gtgctcaaat ttctttagtt gaaattaaga ctggttttaa gttacgctca gctgtgatgg
     3661 gtgacaatca gtgcattact gttttatcag tcttcccctt agagactgac gcagacgagc
     3721 aggaacagag cgccgaagac aatgcagcga gggtggccgc cagcctagca aaagttacaa
     3781 gtgcctgtgg aatcttttta aaacctgatg aaacatttgt acattcaggt tttatctatt
     3841 ttggaaaaaa acaatatttg aatggggtcc aattgcctca gtcccttaaa acggctacaa
     3901 gaatggcacc attgtctgat gcaatttttg atgatcttca agggaccctg gctagtatag
     3961 gcactgcttt tgagcgatcc atctctgaga cacgacatat ctttccttgc aggataaccg
     4021 cagctttcca tacgtttttt tcggtgagaa tcttgcaata tcatcatctc gggttcaata
     4081 aaggttttga ccttggacag ttaacactcg gcaaacctct ggatttcgga acaatatcat
     4141 tggcactagc ggtaccgcag gtgcttggag ggttatcctt cttgaatcct gagaaatgtt
     4201 tctaccggaa tctaggagat ccagttacct caggcttatt ccagttaaaa acttatctcc
     4261 gaatgattga gatggatgat ttattcttac ctttaattgc gaagaaccct gggaactgca
     4321 ctgccattga ctttgtgcta aatcctagcg gattaaatgt ccctgggtcg caagacttaa
     4381 cttcatttct gcgccagatt gtacgcagga ccatcaccct aagtgcgaaa aacaaactta
     4441 ttaatacctt atttcatgcg tcagctgact tcgaagacga aatggtttgt aaatggctat
     4501 tatcatcaac tcctgttatg agtcgttttg cggccgatat cttttcacgc acgccgagcg
     4561 ggaagcgatt gcaaattcta ggatacctgg aaggaacacg cacattatta gcctctaaga
     4621 tcatcaacaa taatacagag acaccggttt tggacagact gaggaaaata acattgcaaa
     4681 ggtggagcct atggtttagt tatcttgatc attgtgataa tatcctggcg gaggctttaa
     4741 cccaaataac ttgcacagtt gatttagcac agattctgag ggaatattca tgggctcata
     4801 ttttagaggg aagacctctt attggagcca cactcccatg tatgattgag caattcaaag
     4861 tgttttggct gaaaccctac gaacaatgtc cgcagtgttc aaatgcaaag caaccaggtg
     4921 ggaaaccatt cgtgtcagtg gcagtcaaga aacatattgt tagtgcatgg ccgaacgcat
     4981 cccgaataag ctggactatc ggggatggaa tcccatacat tggatcaagg acagaagata
     5041 agataggaca acctgctatt aaaccaaaat gtccttccgc agccttaaga gaggccattg
     5101 aattggcgtc ccgtttaaca tgggtaactc aaggcagttc gaacagtgac ttgctaataa
     5161 aaccattttt ggaagcacga gtaaatttaa gtgttcaaga aatacttcaa atgacccctt
     5221 cacattactc aggaaatatt gttcacaggt acaacgatca atacagtcct cattctttca
     5281 tggccaatcg tatgagtaat tcagcaacgc gattgattgt ttctacaaac actttaggtg
     5341 agttttcagg aggtggccag tctgcacgcg acagcaatat tattttccag aatgttataa
     5401 attatgcagt tgcactgttc gatattaaat ttagaaacac tgaggctaca gatatccaat
     5461 ataatcgtgc tcaccttcat ctaactaagt gttgcacccg ggaagtacca gctcagtatt
     5521 taacatacac atctacattg gatttagatt taacaagata ccgagaaaac gaattgattt
     5581 atgacagtaa tcctctaaaa ggaggactca attgcaatat ctcattcgat aatccatttt
     5641 tccaaggtaa acggctgaac attatagaag atgatcttat tcgactgcct cacttatctg
     5701 gatgggagct agccaagacc atcatgcaat caattatttc agatagcaac aattcatcta
     5761 cagacccaat tagcagtgga gaaacaagat cattcactac ccatttctta acttatccca
     5821 agataggact tctgtacagt tttggggcct ttgtaagtta ttatcttggc aatacaattc
     5881 ttcggactaa gaaattaaca cttgacaatt ttttatatta cttaactact caaattcata
     5941 atctaccaca tcgctcattg cgaatactta agccaacatt caaacatgca agcgttatgt
     6001 cacggttaat gagtattgat cctcattttt ctatttacat aggcggtgct gcaggtgaca
     6061 gaggactctc agatgcggcc aggttatttt tgagaacgtc catttcatct tttcttacat
     6121 ttgtaaaaga atggataatt aatcgcggaa caattgtccc tttatggata gtatatccgc
     6181 tagagggtca aaacccaaca cctgtgaata attttctcta tcagatcgta gaactgctgg
     6241 tgcatgattc atcaagacaa caggctttta aaactaccat aagtgatcat gtacatcctc
     6301 acgacaatct tgtttacaca tgtaagagta cagccagcaa tttcttccat gcatcattgg
     6361 cgtactggag gagcagacac agaaacagca accgaaaata cttggcaaga gactcttcaa
     6421 ctggatcaag cacaaacaac agtgatggtc atattgagag aagtcaagaa caaaccacca
     6481 gagatccaca tgatggcact gaacggaatc tagtcctaca aatgagccat gaaataaaaa
     6541 gaacgacaat tccacaagaa aacacgcacc agggtccgtc gttccagtcc tttctaagtg
     6601 actctgcttg tggtacagca aatccaaaac taaatttcga tcgatcgaga cacaatgtga
     6661 aatttcagga tcataactcg gcatccaaga gggaaggtca tcaaataatc tcacaccgtc
     6721 tagtcctacc tttctttaca ttatctcaag ggacacgcca attaacgtca tccaatgagt
     6781 cacaaaccca agacgagata tcaaagtact tacggcaatt gagatccgtc attgatacca
     6841 cagtttattg tagatttacc ggtatagtct cgtccatgca ttacaaactt gatgaggtcc
     6901 tttgggaaat agagagtttc aagtcggctg tgacgctagc agagggagaa ggtgctggtg
     6961 ccttactatt gattcagaaa taccaagtta agaccttatt tttcaacacg ctagctactg
     7021 agtccagtat agagtcagaa atagtatcag gaatgactac tcctaggatg cttctacctg
     7081 ttatgtcaaa attccataat gaccaaattg agattattct taacaactca gcaagccaaa
     7141 taacagacat aacaaatcct acttggttta aagaccaaag agcaaggcta cctaagcaag
     7201 tcgaggttat aaccatggat gcagagacaa cagagaatat aaacagatcg aaattgtacg
     7261 aagctgtata taaattgatc ttacaccata ttgatcctag cgtattgaaa gcagtggtcc
     7321 ttaaagtctt tctaagtgat actgagggta tgttatggct aaatgataat ttagccccgt
     7381 tttttgccac tggttattta attaagccaa taacgtcaag tgctagatct agtgagtggt
     7441 atctttgtct gacgaacttc ttatcaacta cacgtaagat gccacaccaa aaccatctca
     7501 gttgtaaaca ggtaatactt acggcattgc aactgcaaat tcaacgaagc ccatactggc
     7561 taagtcattt aactcagtat gctgactgtg agttacattt aagttatatc cgccttggtt
     7621 ttccatcatt agagaaagta ctataccaca ggtataacct cgtcgattca aaaagaggtc
     7681 cactagtctc tatcactcag cacttagcac atcttagagc agagattcga gaattaacta
     7741 atgattataa tcaacagcga caaagtcgga ctcaaacata tcactttatt cgtactgcaa
     7801 aaggacgaat cacaaaacta gtcaatgatt atttaaaatt ctttcttatt gtgcaagcat
     7861 taaaacataa tgggacatgg caagctgagt ttaagaaatt accagagttg attagtgtgt
     7921 gcaataggtt ctaccatatt agagattgca attgtgaaga acgtttctta gttcaaacct
     7981 tatatttaca tagaatgcag gattctgaag ttaagcttat cgaaaggctg acagggcttc
     8041 tgagtttatt tccggatggt ctctacaggt ttgattgaat taccgtgcat agtatcctga
     8101 tacttgcaaa ggttggttat taacatacag attataaaaa actcataaat tgctctcata
     8161 catcatattg atctaatctc aataaacaac tatttaaata acgaaaggag tccctatatt
     8221 atatactata tttagcctct ctccctgcgt gataatcaaa aagagctcac tagtggatcc
     8281 ctgcagctcg agaggcctaa ttaattaagt cgacgatccg gctgctaaca aagcccgaaa
     8341 ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc
     8401 taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat cgagatcaat
     8461 tctgtgagcg tatggcaaac gaaggaaaaa tagttatagt agccgcactc gatgggacat
     8521 ttcaacgtaa accgtttaat aatattttga atcttattcc attatctgaa atggtggtaa
     8581 aactaactgc tgtgtgtatg aaatgcttta aggaggcttc cttttctaaa cgattgggtg
     8641 aggaaaccga gatagaaata ataggaggta atgatatgta tcaatcggtg tgtagaaagt
     8701 gttacatcga ctcataatat tatatttttt atctaaaaaa ctaaaaataa acattgatta
     8761 aattttaata taatacttaa aaatggatgt tgtgtcgtta gataaaccgt ttatgtattt
     8821 tgaggaaatt gataatgagt tagattacga accagaaagt gcaaatgagg tcgcaaaaaa
     8881 actgccgtat caaggacagt taaaactatt actaggagaa ttattttttc ttagtaagtt
     8941 acagcgacac ggtatattag atggtgccac cgtagtgtat ataggatctg ctcccggtac
     9001 acatatacgt tatttgagag atcatttcta taatttagga gtgatcatca aatggatgct
     9061 aattgacggc cgccatcatg atcctatttt aaatggattg cgtgatgtga ctctagtgac
     9121 tcggttcgtt gatgaggaat atctacgatc catcaaaaaa caactgcatc cttctaagat
     9181 tattttaatt tctgatgtga gatccaaacg aggaggaaat gaacctagta cggcggattt
     9241 actaagtaat tacgctctac aaaatgtcat gattagtatt ttaaaccccg tggcgtctag
     9301 tcttaaatgg agatgcccgt ttccagatca atggatcaag gacttttata tcccacacgg
     9361 taataaaatg ttacaacctt ttgctccttc atattcaggg ccgtcgtttt acaacgtcgt
     9421 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     9481 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     9541 aatggcgaat ggcgcgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     9601 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     9661 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     9721 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     9781 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     9841 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     9901 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     9961 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat ttcccaggtg
    10021 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
    10081 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
    10141 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
    10201 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
    10261 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
    10321 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
    10381 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
    10441 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
    10501 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
    10561 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
    10621 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
    10681 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
    10741 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
    10801 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
    10861 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
    10921 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
    10981 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
    11041 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
    11101 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
    11161 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
    11221 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
    11281 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
    11341 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
    11401 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
    11461 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
    11521 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
    11581 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
    11641 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
    11701 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
    11761 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
    11821 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
    11881 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
    11941 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
    12001 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
    12061 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
    12121 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcc
//
