LOCUS       sequence-131093-        7978 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes for Ebola Virus Zaire NP under control of a T7 RNA 
            polymerase promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7978)
  AUTHORS   Trunschke M, Conrad D, Enterlein S, Olejnik J, Brauburger K, 
            Muhlberger E
  TITLE     The L-VP35 and L-L interaction domains reside in the amino terminus 
            of the Ebola virus L protein and are potential targets for 
            antivirals.
  JOURNAL   Virology. 2013 Jul 5;441(2):135-45. doi: 
            10.1016/j.virol.2013.03.013. Epub 2013 Apr 11.
  PUBMED    23582637
REFERENCE   2  (bases 1 to 7978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.virol.2013.03.013"; journalName: "Virology"; date: 
            "2013-07-5- 5"; volume: "441"; issue: "2"; pages: "135-45"
COMMENT     Sequence Label: pTM1_NP_ZEBOV
FEATURES             Location/Qualifiers
     source          1..7978
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     794..813
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        794..812
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    851..1423
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1018..1035)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1245..1264
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3022..3033
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4163..4181)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      4177..4224
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(5204..5226)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5356..5811
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5443..5462)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5653..5674
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5840..5944
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5945..6013,6014..6805)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6163..6182)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6976..7564
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7465..7484
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7718..7735
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7852..7873
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7888..7893,7894..7911,7912..7918)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7926..7942
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7931..7953
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7950..7966
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7950..7966
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttttgc gatcaataaa tggatcacaa ccagtatctc ttaacgatgt tcttcgcaga
       61 tgatgattca ttttttaagt atttggctag tcaagatgat gaatcttcat tatctgatat
      121 attgcaaatc actcaatatc tagactttct gttattatta ttgatccaat caaaaaataa
      181 attagaagcc gtgggtcatt gttatgaatc tctttcagag gaatacagac aattgacaaa
      241 attcacagac tttcaagatt ttaaaaaact gtttaacaag gtccctattg ttacagatgg
      301 aagggtcaaa cttaataaag gatatttgtt cgactttgtg attagtttga tgcgattcaa
      361 aaaagaatcc tctctagcta ccaccgcaat agatcctgtt agatacatag atcctcgtcg
      421 caatatcgca ttttctaacg tgatggatat attaaagtcg aataaagtga acaataatta
      481 attctttatt gtcatcatga acggcggaca tattcagttg ataatcggcc ccatgttttc
      541 aggtaaaagt acagaattaa ttagacgagt tagacgttat caaatagctc aatataaatg
      601 cgtgactata aaatattcta acgataatag atacggaacg ggactatgga cgcatgataa
      661 gaataatttt gaagcattgg aagcaactaa actatgtgat gtcttggaat caattacaga
      721 tttctccgtg ataggtatcg atgaaggaca gttctttcca gacattgttg aattgatctc
      781 gatcccgcga aattaatacg actcactata gggagaccac aacggtttcc ctctagcggg
      841 atcaattccg cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat
      901 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg
      961 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc
     1021 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt
     1081 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg
     1141 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac
     1201 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg
     1261 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg
     1321 ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc
     1381 cgaaccacgg ggacgtggtt ttcctttgaa aaacacgata ataccatggg aattccccgg
     1441 ggagctcact agtggatcca caaaattccg agtatggatt ctcgtcctca gaaaatctgg
     1501 atggcgccga gtctcactga atctgacatg gattaccaca agatcttgac agcaggtctg
     1561 tccgttcaac aggggattgt tcggcaaaga gtcatcccag tgtatcaagt aaacaatctt
     1621 gaagaaattt gccaacttat catacaggcc tttgaagcag gtgttgattt tcaagagagt
     1681 gcggacagtt tccttctcat gctttgtctt catcatgcgt accagggaga ttacaaactt
     1741 ttcttggaaa gtggcgcagt caagtatttg gaagggcacg ggttccgttt tgaagtcaag
     1801 aagcgtgatg gagtgaagcg ccttgaggaa ttgctgccag cagtatctag tggaaaaaac
     1861 attaagagaa cacttgctgc catgccggaa gaggagacaa ctgaagctaa tgccggtcag
     1921 tttctctcct ttgcaagtct attccttccg aaattggtag taggagaaaa ggcttgcctt
     1981 gagaaggttc aaaggcaaat tcaagtacat gcagagcaag gactgataca atatccaaca
     2041 gcttggcaat cagtaggaca catgatggtg attttccgtt tgatgcgaac aaattttctg
     2101 atcaaatttc tcctaataca ccaagggatg cacatggttg ccgggcatga tgccaacgat
     2161 gctgtgattt caaattcagt ggctcaagct cgtttttcag gcttattgat tgtcaaaaca
     2221 gtacttgatc atatcctaca aaagacagaa cgaggagttc gtctccatcc tcttgcaagg
     2281 accgccaagg taaaaaatga ggtgaactcc tttaaggctg cactcagctc cctggccaag
     2341 catggagagt atgctccttt cgcccgactt ttgaaccttt ctggagtaaa taatcttgag
     2401 catggtcttt tccctcaact atcggcaatt gcactcggag tcgccacagc acacgggagt
     2461 accctcgcag gagtaaatgt tggagaacag tatcaacaac tcagagaggc tgccactgag
     2521 gctgagaagc aactccaaca atatgcagag tctcgcgaac ttgaccatct tggacttgat
     2581 gatcaggaaa agaaaattct tatgaacttc catcagaaaa agaacgaaat cagcttccag
     2641 caaacaaacg ctatggtaac tctaagaaaa gagcgcctgg ccaagctgac agaagctatc
     2701 actgctgcgt cactgcccaa aacaagtgga cattacgatg atgatgacga cattcccttt
     2761 ccaggaccca tcaatgatga cgacaatcct ggccatcaag atgatgatcc gactgactca
     2821 caggatacga ccattcccga tgtggtggtt gatcccgatg atggaagcta cggcgaatac
     2881 cagagttact cggaaaacgg catgaatgca ccagatgact tggtcctatt cgatctagac
     2941 gaggacgacg aggacactaa gccagtgcct aatagatcga ccaagggtgg acaacagaag
     3001 aacagtcaaa agggccagca tatagagggc agacagacac aatccaggcc aattcaaaat
     3061 gtcccaggcc ctcacagaac aatccaccac gccagtgcgc cactcacgga caatgacaga
     3121 agaaatgaac cctccggctc aaccagccct cgcatgctga caccaattaa cgaagaggca
     3181 gacccactgg acgatgccga cgacgagacg tctagccttc cgcccttgga gtcagatgat
     3241 gaagagcagg acagggacgg aacttccaac cgcacaccca ctgtcgcccc accggctccc
     3301 gtatacagag atcactctga aaagaaagaa ctcccgcaag acgagcaaca agatcaggac
     3361 cacactcaag aggccaggaa ccaggacagt gacaacaccc agtcagaaca ctcttttgag
     3421 gagatgtatc gccacattct aagatcacag gggccatttg atgctgtttt gtattatcat
     3481 atgatgaagg atgagcctgt agttttcagt accagtgatg gcaaagagta cacgtatcca
     3541 gactcccttg aagaggaata tccaccatgg ctcactgaaa aagaggctat gaatgaagag
     3601 aatagatttg ttacattgga tggtcaacaa ttttattggc cggtgatgaa tcacaagaat
     3661 aaattcatgg caatcctgca acatcatcag tgaatgagca tggaacaatg ggatgattca
     3721 accgacaaat agctaacatt aagtagtcaa ggaacgaaaa caggaagaat ttttgatgtc
     3781 taaggtgtga attattatca caataaaagt gattcttatt tttgaattta aagctagctt
     3841 attattacta gccgtttttc aaagttcaat ttgagtctta atgcaaatag gcgttaagcc
     3901 acagttatag ccataattgt aactcaatat tctaactagc gatttatcta aattaaatta
     3961 cattatgctt ttataactta cctactagcc tgcccaacat ttacacgatc gttttataat
     4021 taagaaaaaa ctaatgatga agattaaaac cttcatcatc cttacgtcaa ttgaattctc
     4081 tagcactcga agctcctaat taattaagtc gacgatccgg ctgctaacaa agcccgaaag
     4141 gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct
     4201 aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggatc gagatcaatt
     4261 ctgtgagcgt atggcaaacg aaggaaaaat agttatagta gccgcactcg atgggacatt
     4321 tcaacgtaaa ccgtttaata atattttgaa tcttattcca ttatctgaaa tggtggtaaa
     4381 actaactgct gtgtgtatga aatgctttaa ggaggcttcc ttttctaaac gattgggtga
     4441 ggaaaccgag atagaaataa taggaggtaa tgatatgtat caatcggtgt gtagaaagtg
     4501 ttacatcgac tcataatatt atatttttta tctaaaaaac taaaaataaa cattgattaa
     4561 attttaatat aatacttaaa aatggatgtt gtgtcgttag ataaaccgtt tatgtatttt
     4621 gaggaaattg ataatgagtt agattacgaa ccagaaagtg caaatgaggt cgcaaaaaaa
     4681 ctgccgtatc aaggacagtt aaaactatta ctaggagaat tattttttct tagtaagtta
     4741 cagcgacacg gtatattaga tggtgccacc gtagtgtata taggatctgc tcccggtaca
     4801 catatacgtt atttgagaga tcatttctat aatttaggag tgatcatcaa atggatgcta
     4861 attgacggcc gccatcatga tcctatttta aatggattgc gtgatgtgac tctagtgact
     4921 cggttcgttg atgaggaata tctacgatcc atcaaaaaac aactgcatcc ttctaagatt
     4981 attttaattt ctgatgtgag atccaaacga ggaggaaatg aacctagtac ggcggattta
     5041 ctaagtaatt acgctctaca aaatgtcatg attagtattt taaaccccgt ggcgtctagt
     5101 cttaaatgga gatgcccgtt tccagatcaa tggatcaagg acttttatat cccacacggt
     5161 aataaaatgt tacaaccttt tgctccttca tattcagggc cgtcgtttta caacgtcgtg
     5221 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     5281 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     5341 atggcgaatg gcgcgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     5401 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     5461 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     5521 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     5581 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     5641 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     5701 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     5761 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt tcccaggtgg
     5821 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     5881 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     5941 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     6001 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     6061 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     6121 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     6181 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     6241 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     6301 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     6361 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     6421 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     6481 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     6541 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     6601 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     6661 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     6721 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     6781 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     6841 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     6901 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     6961 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     7021 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     7081 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     7141 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     7201 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     7261 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     7321 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     7381 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     7441 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     7501 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     7561 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     7621 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     7681 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     7741 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     7801 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     7861 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     7921 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgcc
//
