LOCUS       sequence-131095-         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology 
            arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon 
            based on Bean Yellow Dwarf Virus; sgRNA= 7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Cermak T, Baltes NJ, Cegan R, Zhang Y, Voytas DF
  TITLE     High-frequency, precise modification of the tomato genome.
  JOURNAL   Genome Biol. 2015 Nov 6;16(1):232. doi: 10.1186/s13059-015-0796-9.
  PUBMED    26541286
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2015-11-6"; volume: "16(1):232. doi"; pages: " 
            10.1186/s13059-015-0796-9"
COMMENT     Sequence Label: pTC223
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(508..756)
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
ORIGIN
        1 gctatttcta gctctaaaac tctacaaatt ataaacgaca atcactactt cgactctagc
       61 tgtatataaa ctcagcttcg ttttcttatc taagcgatgt gggacttttg aagattgttt
      121 tcaacttaaa tgggcctata taagaaatac tattgttctt tcccatataa atgggcctgc
      181 ttctcttctt tcagattccc aggggccttt tgaagattat cttcatatct taagaatgaa
      241 gatgttttat tcaatcaaat tcttgaaggt tcgatgccta atcattctaa tcctgggaca
      301 aactatgaaa caagatacaa aaactccgaa tggaaagtta aaaagaagaa aacgaaagct
      361 acggttcaag aaaatgtaag ctgataaaca aaaaaaaact gtatgaacga agaagaagaa
      421 aaaaagctaa gaagaaatga tgtattgtgc ggaaggcaag tcgagtttcc gttgttcaac
      481 gaagccatgg gtccgtcacc ggagctcctt atctttaatc atattccata gtccatacca
      541 tagcacatac agtagttata tgctgcagaa gagatccaac aaaacattca caatggatta
      601 tagaaacatt tgtttattca ttataatgtg atcttacatt catttaatat tagaaaaagc
      661 cacaaattca taacacaaca agccaagaaa aaaacacaaa cttaagcaca caagcttttt
      721 atttgacaca ccaaatattt catcttcatc ttcatatctc gagtcaaacc ttcctcttct
      781 tctta
//
