LOCUS       sequence-13110-e        3750 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 3750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1490-HQ858754
FEATURES             Location/Qualifiers
     source          1..3750
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             112..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(1307..1406)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1423..1442)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1424..1442)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1447..1463)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1447..1463)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1565..1584)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1576..2385
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1643..1662)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2532..3120
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3021..3040
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3274..3291
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3450..3477)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3569..3655)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3704..3726
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3718..3735
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3719..3735
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc cttgtttaac
      121 tttaagaagg agcccttcac catggggaag gcggaggcga ggtcaagggg gcgcggcggc
      181 ggcggcggcg gcggaggcgc cagcggcaat ccggggaaga tcttcgtcgg agggctcccg
      241 cgggacacca cggatgctac atttgtgagg cattttggac agtatggaga aatagttgat
      301 tcggtgataa tgaaggatag gcacacatct cagccacggg gttttggctt cattacatac
      361 tctgaccctg ctgttgttga taaagtgata gaggacaatc atgttatcaa tggaaagcaa
      421 gttgaaatta agagaaccat accaaaaggt tcggtgcaat ctagctctaa ggatttcaag
      481 actaaaaaga tatttgttgg tgggcttccg tcaacactaa cagaagacga attcaagagt
      541 ttctttgcaa gatatgggac tgtggtggac caccagatta tgtttgatca tgaaaccaaa
      601 cgttcccgag gctttggatt tatagttttt gcttctgagc aagttgtaga tgatttgtta
      661 gcaaatggaa atatggttga ccttgctggt tcaaaggtgg agatcaagaa agcagaacca
      721 aagaaatcct ccaatgcacc gccaccacca gtgcatgtta gaaatgctag gtcagcatat
      781 gatagcggct ctagagacca cccttcagct gacaactatg gtggaatggc cagtgcttat
      841 ggaaactaca ggggtggtgg atttggtcct tataggagcg atacaggatt tggtgctagg
      901 ctcagcagct atggtggggt aggtgatttt ggtggtgcat atggtcgtta ctatgcagga
      961 ttaggagggg gatatggagc cgcgtcttcg tttggttatc ctggtcgctt tgggttatat
     1021 ggaggaggtt ttggtggatc ttatgctggt gctgacttaa gtggctatag acgcggtgga
     1081 ggtgatgaga gcttcggtgc ccctggtggt aatcctgggt ttggtggtga tgctgaagag
     1141 agctttggtg gccctagtag ttctggcttt agtggcaccg cctatggtgg ggcatatgat
     1201 cctacgctag gtggttatgg atctgctagc acacctgaca tgaatagggg aagcttcact
     1261 ggaggatatg gacgatatca cccatatgga aagggtgggc gcgccgaccc agctttcttg
     1321 tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga acaggtcact
     1381 atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag tgagtcgtat
     1441 tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac
     1501 attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt
     1561 aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg attaaattcc
     1621 aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt
     1681 gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct gaaacatggc
     1741 aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa
     1801 tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc
     1861 accactgcga tccccggaaa aacagcattc caggtattag aagaatatcc tgattcaggt
     1921 gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt
     1981 aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat
     2041 aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa
     2101 gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt cactcatggt
     2161 gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt
     2221 ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt
     2281 gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat
     2341 atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat
     2401 tggttgtaac actggcagag cattacgctg acttgacggg acggcgcaag ctcatgacca
     2461 aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc
     2521 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     2581 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     2641 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     2701 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     2761 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     2821 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     2881 gagcgaacga cctacaccga actgagatac ctacagcgtg agcattgaga aagcgccacg
     2941 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     3001 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     3061 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     3121 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     3181 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     3241 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     3301 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     3361 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc
     3421 gctagccagg aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct
     3481 tagtttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct
     3541 tcacaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca
     3601 aacaacagat aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc
     3661 tggcagttcc ctactctcgc gttaacgcta gcatggatgt tttcccagtc acgacgttgt
     3721 aaaacgacgg ccagtcttaa gctcgggccc
//
