LOCUS       sequence-131102-         736 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Multisite gateway entry clone for adding C-terminal fusions of OSBP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Ariotti N, Hall TE, Rae J, Ferguson C, McMahon KA, Martel N, Webb 
            RE, Webb RI, Teasdale RD, Parton RG
  TITLE     Modular Detection of GFP-Labeled Proteins for Rapid Screening by 
            Electron Microscopy in Cells and Organisms.
  JOURNAL   Dev Cell. 2015 Nov 23;35(4):513-25. doi: 
            10.1016/j.devcel.2015.10.016. Epub 2015 Nov 12.
  PUBMED    26585296
REFERENCE   2  (bases 1 to 736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2015.10.016"; journalName: "Dev Cell"; date: 
            "2015-11-23- 23"; volume: "35"; issue: "4"; pages: "513-25"
COMMENT     Sequence Label: p3E-OSBP
FEATURES             Location/Qualifiers
     source          1..736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(32..155)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(458..553)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(590..609)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(591..609)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(614..630)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(614..630)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 caggtcacta ataccatcta agtagttggt tcatagtgac tgcatatgtt gtgttttaca
       61 gtattatgta gtctgttttt tatgcaaaat ctaatttaat atattgatat ttatatcatt
      121 ttacgtttct cgttcaactt tcttgtacaa agtggcttcg ggctcggctc gagagggctg
      181 gctcttcaaa tggaccaatt atatcaaagg ctaccagcgg cgatggttcg tgctgagcaa
      241 cgggctcctg agctactaca gatcaaaggc agagatgaga catacctgcc gtggtaccat
      301 caacctcgcc acagccaaca tcaccgtgga ggactcctgc aacttcatca tttccaatgg
      361 gggtgctcag acctaccatc tgaaagctag ttcagaagtt gagcggcagc gctgggtgac
      421 ggccctggaa ctggccaagg ccaaagctgt gaagtgacaa ctttattata caaagttggc
      481 attataaaaa agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat
      541 aaaatcatta tttggagctc catggtagcg ttaacgcggc cgcgatatcc cctatagtga
      601 gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg
      661 atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat
      721 aaacagtaat acaagg
//
