LOCUS       sequence-13111-e        3504 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1497-HQ858755
FEATURES             Location/Qualifiers
     source          1..3504
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             112..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(1061..1160)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1177..1196)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1178..1196)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1201..1217)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1201..1217)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1319..1338)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1330..2139
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1397..1416)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2286..2874
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2775..2794
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3028..3045
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3204..3231)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3323..3409)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3458..3480
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3472..3489
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3473..3489
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc cttgtttaac
      121 tttaagaagg agcccttcac catggacccg atggacatcg tcggcaagtc caaggaggac
      181 gtctccctcc ccaaatcaac aatgtttaag ataattaagg agatgcttcc tcctgatgta
      241 cgagtggcaa gagatgcaca ggatcttctt gttgaatgct gtgtagagtt catcaatctc
      301 ctttcgtctg aatcgaatga agtgtgcagc agagaagaga aaaaaacgat tgctcctgag
      361 catgttatca aggctctaag tgatcttggc ttcagagagt acattgagga ggtttatgct
      421 gcatatgaac agcacaaact tgatactttg gattctccaa aagctggcaa attcactggg
      481 attgagatga cagaggaaga agctgttgct gagcaacaaa ggatgtttgc tgaggctcga
      541 gcaaggatga ataatggagc ccccaaacca aaggagacag aacaagagcc gccacagcaa
      601 ccacaagcac aaccccaact tcagctgcat actgaaccac agcaacccgt gcaatctcaa
      661 gtgcagttgc attcgccaac acagcattcc ctccaacctc aagtgcagct gcacccccag
      721 ccacaacagc tcccacaagt gcaggtgcac tctcagacgc agctgcaccc tcagccacag
      781 cagccccaag tgcaggtcca tccgcagctg cagcaactcc cacaattaca ggcccactcg
      841 cagccaccgc aaccccaagt gcaaatccac ccacagccgc agcagccccc acaagtgcag
      901 ttgcagtcat cagtccagca gacctcccaa ccccaacctc aggtacatct ctacaatcac
      961 agggggggct cacaagcgca gctgcagccc caactgccag gtcagctgca gacacagggg
     1021 caaaccggac ctggcattga cagtaagggt gggcgcgccg acccagcttt cttgtacaaa
     1081 gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt
     1141 caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc gtattacatg
     1201 gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca
     1261 caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca
     1321 aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg
     1381 gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca
     1441 atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt
     1501 agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg
     1561 cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact
     1621 gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat
     1681 attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt
     1741 ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt
     1801 ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg
     1861 aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca tggtgatttc
     1921 tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga
     1981 gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt
     2041 tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat
     2101 aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt taattggttg
     2161 taacactggc agagcattac gctgacttga cgggacggcg caagctcatg accaaaatcc
     2221 cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     2281 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     2341 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     2401 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     2461 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     2521 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2581 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2641 acgacctaca ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc
     2701 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2761 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2821 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     2881 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     2941 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     3001 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     3061 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     3121 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc
     3181 caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt
     3241 gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa
     3301 cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac
     3361 agataaaacg aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag
     3421 ttccctactc tcgcgttaac gctagcatgg atgttttccc agtcacgacg ttgtaaaacg
     3481 acggccagtc ttaagctcgg gccc
//
