LOCUS       sequence-131112-         729 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes a shRNA against the protease ZMPSTE24.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Moiseeva O, Lessard F, Acevedo-Aquino M, Vernier M, Tsantrizos YS, 
            Ferbeyre G
  TITLE     Mutant lamin A links prophase to a p53 independent senescence 
            program.
  JOURNAL   Cell Cycle. 2015 Aug 3;14(15):2408-21. doi: 
            10.1080/15384101.2015.1053671. Epub 2015 Jun 1.
  PUBMED    26029982
REFERENCE   2  (bases 1 to 729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1080/15384101.2015.1053671"; journalName: "Cell Cycle"; date: 
            "2015-08-3- 3"; volume: "14"; issue: "15"; pages: "2408-21"
COMMENT     Sequence Label: mLPx-shZMPSTE24
FEATURES             Location/Qualifiers
     source          1..729
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           complement(1..127)
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(154..168)
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(196..316)
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(372..388)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(404..426)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 aaagtgattt aatttatacc attttaattc agctttgtaa aaatgtatca aagagatagc
       61 aaggtattca gttttagtaa acaagataat tgctcctaaa gtagcccctt gaattccgag
      121 gcagtaggca gatcatggat tctgaaacat ttacatctgt ggcttcacta aatgtttcat
      181 gaatccatga tgcgctcact gtcaacagca tataccttct cgagccttct gttgggttaa
      241 cctgaagaag taatcccagc aagtgtttcc aagatgtgca ggcaacgatt ctgtaaagta
      301 ctgaagcctc attcaaacaa ttaccctgtt atccctagtc gagagatcta attccggcgc
      361 ctagagaagg agtgagggct ggataaaggg aggatcgagg cggggtcgaa cgaggaggtt
      421 caagggggag agacggggcg gatggaggaa gaggaggcgg aggcttaggg tgtacaaagg
      481 gcttgaccca gggagggggg tcaaaagcca aggcttccca ggtcacgatg taggggacct
      541 ggtctgggtg tccatgcggg ccaggtgaaa agaccttgat cttaacctgg gtgatgaggt
      601 ctcggttaaa ggtgccgtct cgcggccatc cgacgttaaa ggttggccat tctgcagagc
      661 agaaggtaac ccaacgtctc ttcttgacat ctaccgactg gttgtgagcg atccgctcga
      721 catctttcc
//
