LOCUS       sequence-131113-         724 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Zebrafish expression vector for heat-shock inducible expression of 
            APEX2-GBP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Ariotti N, Hall TE, Rae J, Ferguson C, McMahon KA, Martel N, Webb 
            RE, Webb RI, Teasdale RD, Parton RG
  TITLE     Modular Detection of GFP-Labeled Proteins for Rapid Screening by 
            Electron Microscopy in Cells and Organisms.
  JOURNAL   Dev Cell. 2015 Nov 23;35(4):513-25. doi: 
            10.1016/j.devcel.2015.10.016. Epub 2015 Nov 12.
  PUBMED    26585296
REFERENCE   2  (bases 1 to 724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2015.10.016"; journalName: "Dev Cell"; date: 
            "2015-11-23- 23"; volume: "35"; issue: "4"; pages: "513-25"
COMMENT     Sequence Label: HSP70l-APEX2-GBP in pDEST-Tol2-pA2-acrys-mCherry
FEATURES             Location/Qualifiers
     source          1..724
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(127..151)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             156..497
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid 
                     antibody (Rothbauer et al., 2008)"
                     /label=GFP nanobody
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
     protein_bind    complement(501..521)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(529..545)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    561..695
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(585..604)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     639..658
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 aggctctttt gtctgaccct gtattccgcc ctctcgttga caaatatgca gcggacgaag
       61 atgccttctt tgctgattac gctgaggctc accaaaagct ttccgagctt gggtttgctg
      121 atgccgaccc agctttcttg tacaaagtgg tgcaggtgca gctggtggag tctggaggag
      181 ctctggtgca gcctggagga agcctgcgcc tgagctgtgc agctagcgga tttcctgtga
      241 accgctacag catgcgctgg taccgccagg ctcctggtaa agagcgcgag tgggtggctg
      301 gaatgagcag cgctggagat cgcagcagct acgaggacag cgtgaaagga cgctttacaa
      361 tcagccgcga tgatgctcgc aacacagtgt acctgcagat gaactctctg aaacctgagg
      421 acactgctgt gtactactgt aacgtgaacg tgggtttcga gtactgggga cagggaacac
      481 aggtgacagt gagctcttga caactttatt atacatagtt gataattcac tggccgtcgt
      541 tttacggtac catcgatgat gatccagaca tgataagata cattgatgag tttggacaaa
      601 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
      661 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
      721 tgtt
//
