LOCUS       sequence-131117-         723 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Multisite gateway entry clone for adding C-terminal fusions of OSBP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Ariotti N, Hall TE, Rae J, Ferguson C, McMahon KA, Martel N, Webb 
            RE, Webb RI, Teasdale RD, Parton RG
  TITLE     Modular Detection of GFP-Labeled Proteins for Rapid Screening by 
            Electron Microscopy in Cells and Organisms.
  JOURNAL   Dev Cell. 2015 Nov 23;35(4):513-25. doi: 
            10.1016/j.devcel.2015.10.016. Epub 2015 Nov 12.
  PUBMED    26585296
REFERENCE   2  (bases 1 to 723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2015.10.016"; journalName: "Dev Cell"; date: 
            "2015-11-23- 23"; volume: "35"; issue: "4"; pages: "513-25"
COMMENT     Sequence Label: p3E-OSBP
FEATURES             Location/Qualifiers
     source          1..723
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    19..114
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    417..540
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(611..628)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(611..627)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(620..642)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 acgctaccat ggagctccaa ataatgattt tattttgact gatagtgacc tgttcgttgc
       61 aacaaattga taagcaatgc ttttttataa tgccaacttt gtataataaa gttgtcactt
      121 cacagctttg gccttggcca gttccagggc cgtcacccag cgctgccgct caacttctga
      181 actagctttc agatggtagg tctgagcacc cccattggaa atgatgaagt tgcaggagtc
      241 ctccacggtg atgttggctg tggcgaggtt gatggtacca cggcaggtat gtctcatctc
      301 tgcctttgat ctgtagtagc tcaggagccc gttgctcagc acgaaccatc gccgctggta
      361 gcctttgata taattggtcc atttgaagag ccagccctct cgagccgagc ccgaagccac
      421 tttgtacaag aaagttgaac gagaaacgta aaatgatata aatatcaata tattaaatta
      481 gattttgcat aaaaaacaga ctacataata ctgtaaaaca caacatatgc agtcactatg
      541 aaccaactac ttagatggta ttagtgacct gtagaattcg agctctagag ctgcagggcc
      601 cgagcttaag actggccgtc gttttacaac gtcgtgactg ggaaaacatc catgctagcg
      661 ttaacgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca gtcggaagac
      721 tgg
//
