LOCUS       sequence-131118-         944 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  when used to produce lentivirus, express physiological levels of 
            insert .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, 
            Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN,
            Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, 
            McDonnell DP, Sabatini DM, Wood KC
  TITLE     Systematic identification of signaling pathways with potential to 
            confer anticancer drug resistance.
  JOURNAL   Sci Signal. 2014 Dec 23;7(357):ra121. doi: 
            10.1126/scisignal.aaa1877.
  PUBMED    25538079
REFERENCE   2  (bases 1 to 944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2014-12-23"; volume: "7(357):ra121. doi"; pages: " 
            10.1126/scisignal.aaa1877"
COMMENT     Sequence Label: JNK2 WT O/E (MAPK9)-pcw107-V5
FEATURES             Location/Qualifiers
     source          1..944
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(58..99)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    107..131
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atctttccaa attttgtaat ccagaggttg attgtcgact taacgcggtc attactacgt
       61 agaatcgaga ccgaggagag ggttagggat aggcttacca ccggtaacca ctttgtacaa
      121 gaaagttggc aatcgacagc cttcaagggg tcccgtcgag gcatcaagac tgctgtctgt
      181 gtctgaggcc agcgtctgct cagtggacat ggatgaaatg tcattgatcg atgaagactg
      241 agaaggagtg gcgttgctac ttactgctgc atctgaaggc tgatctttta caacaccatt
      301 cttgcttctt tcttcccaat ccatgacttc tttgtaaatt agctctttcc attcttcaat
      361 tgcatgttct ctttcttcca actgggcatc ataaatttga ggtggtgggg cttctgcttc
      421 ggcggggtca taccaaacag tgatgtatgg gtgacgcaga gcttcgtcta cagagatccg
      481 cttgtcagga tcaatcacta acatttttga taacagatct ctggcttgac ttgtttttat
      541 tttgtctcgc tcagattctg atgggaatat ccaatctgga aagagttctt caaatttgat
      601 tccaggatac tttggtctgt tttcgacata attcctcaca gttggctgaa gtttcttcat
      661 gaactctgct gatggtgttc ccagctgctc aataacttta ttccactgat caatatggtc
      721 agtgccttgg aatatcacac aacctttcac cagctctccc atgatgcaac ccactgacca
      781 gatatcaacg ttctctttgt agcccatacc caggatgact tcgggcgccc ggtagtaccg
      841 tgtcaccacg taaggggtca tcatgaagtt agtgcacgct gtccgggcca ggccaaagtc
      901 aaggatcttc agggtgcagt ctgatttcac aacaatgttg ctag
//
