LOCUS       sequence-13113-e        4215 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 4215)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1499-HQ858757
FEATURES             Location/Qualifiers
     source          1..4215
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             112..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1687..1698
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(1772..1871)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1888..1907)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1889..1907)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1912..1928)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1912..1928)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2030..2049)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2041..2850
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2108..2127)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2997..3585
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3486..3505
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3739..3756
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3915..3942)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4034..4120)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4169..4191
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4183..4200
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4184..4200
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc cttgtttaac
      121 tttaagaagg agcccttcac catgcctgcg cccgtcgcac ctgtaacctc caggctgaag
      181 cgggaagact gcccccgcac caagcacgac tccctctttt ccccatggaa ggtccttgtc
      241 gggccgtcgg actggaggga ccacgccgcc ggcaaggaag gggtccagag gtaccgcatc
      301 cgcaacctcc cggacaactt ccccggcctc tacgagctag gcgttgcctg cgcggccgac
      361 gagggcgtca ggtcccggag gcgtgacgcg cgaggcgtcg tcgtggtgta cctcggccag
      421 gccgacagtg tccgggcgag gctgcagcag tatggccgct cggggtcgca tctggacact
      481 acgaactcgc cgggctcttc tgatagagat gcggcgaatg cgctcgccga gggtcctgga
      541 ctcttcagag aagtgttctc cagaggctac tccgtcgtgt ttcgatgtgc gctgatggac
      601 aataaacaag aagctgagaa gactgaagca ctactcctga gagtatttga ttatgcatgg
      661 aacaagcttc agaatggtgc ttgtcgccgg caagaaatac tgcataagtt agaactgcga
      721 gcagccaacc ataggtctct acttagcaga gtaagtgaca tgaaacaaaa aatatttgga
      781 cagaaagcag gtataaatat aaagggaagt gggccagcta acacctcgcc tagcataatt
      841 aaaagtatgc tcccaagggt tcgtacaatc gttcgtttca gacctcagtt agtaaaccct
      901 aacaacagtg aaggtgggat aactggtatt ccctatgcaa atagacaagc acatagaagg
      961 aagtccaaag gataccaagt aaaaaagttc gatgttacaa aacggagaac tgtgccgata
     1021 catgattcta actctgtctg tggagtgctg ctggaagatg gtttttgtct ggagcaccca
     1081 gttcatggaa gaaagaggtg cagcttacac aaaggtaaaa gagtcaaagg cagcccaaaa
     1141 agttcgccta ctagccatcc ttgccaagtt gggattgcaa ttactgagtt ttgcctaact
     1201 gaagatttgg acagctcggt cccaaaacag gaaagcgaaa tacggcccaa aaatatgtct
     1261 acaactctga aagaacctca gaggcaaagc gataatttgg aatcacaaaa ggtgaatact
     1321 ggagaagctt ctacagaaga tggaacacaa gaagtttcta gggatgcttg tatcttagaa
     1381 gagaaggcct tcttccatgc tgactctgag tctcaggagc agcagccttc tgggagaatg
     1441 tggtttgagc tgctcaaatc acagaggaaa ccggcgagcg cacatccatc aagaagaggc
     1501 ttgggatctc agataacagc aatgacagat aacaggtcct gcaaaatggt gtcaattgca
     1561 ggaatggaaa ggactgagaa ggacagtgga gtagaggtca ctgctgcttc gttttccaga
     1621 agctcaggat ggccctgtac ctgtggcgcc cgtacgtcgg acggttcacg ttgtatgaat
     1681 atgccgattg aagggaggaa gagatgtgca ttgcacaaag ggcaaagggc agcattcccc
     1741 cctacaccat cggcaaaggg tgggcgcgcc gacccagctt tcttgtacaa agttggcatt
     1801 ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa
     1861 atcattattt gccatccagc tgatatcccc tatagtgagt cgtattacat ggtcatagct
     1921 gtttcctggc agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa
     1981 aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt
     2041 atgagccata ttcaacggga aacgtcgagg ccgcgattaa attccaacat ggatgctgat
     2101 ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcgc
     2161 ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc
     2221 aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg
     2281 accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc
     2341 ggaaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat
     2401 gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac
     2461 agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat
     2521 gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg
     2581 cataaacttt tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat
     2641 aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc
     2701 gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca
     2761 ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag
     2821 tttcatttga tgctcgatga gtttttctaa tcagaattgg ttaattggtt gtaacactgg
     2881 cagagcatta cgctgacttg acgggacggc gcaagctcat gaccaaaatc ccttaacgtg
     2941 agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     3001 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     3061 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     3121 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     3181 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     3241 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     3301 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     3361 accgaactga gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga
     3421 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     3481 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     3541 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     3601 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     3661 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     3721 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     3781 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     3841 gactggaaag cgggcagtga gcgcaacgca attaatacgc gtaccgctag ccaggaagag
     3901 tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg
     3961 cagtttatgg cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat
     4021 ccgctcccgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac
     4081 gaaaggccca gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact
     4141 ctcgcgttaa cgctagcatg gatgttttcc cagtcacgac gttgtaaaac gacggccagt
     4201 cttaagctcg ggccc
//
