LOCUS       sequence-131132-       11291 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the extracellular region of the Dscaml1 protein, 
            C-terminally fused to alkaline phosphatase + 6X histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11291)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 11291)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Dscaml1-AP-His
FEATURES             Location/Qualifiers
     source          1..11291
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             6758..6775
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    6801..6922
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6838..6857)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6892..6911
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6960..6977)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7131..7719)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7211..7230)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7890..8681,8682..8750))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8513..8532
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8751..8855)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8923..8941
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8979..9001)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9003..9023)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9008..9338
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9189..9324
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9251..9270
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     9475..9494
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(9561..10016)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9698..9719)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9910..9929
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10142..10164
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10156..10173
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10157..10173
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        10203..10586
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10587..10790
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10741..10761
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     10787..10811
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          join(10926..11291,1..460)
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
ORIGIN
        1 ggatctccac gcgaatctcg ggtacgtgtt ccggacatgg gctcttctcc ggtagcggcg
       61 gagcttccac atccgagccc tggtcccatg cctccagcgg ctcatggtcg ctcggcagct
      121 ccttgctcct aacagtggag gccagactta ggcacagcac aatgcccacc accaccagtg
      181 tgccgcacaa ggccgtggcg gtagggtatg tgtctgaaaa tgagctcgga gattgggctc
      241 gcaccgctga cgcagatgga agacttaagg cagcggcaga agaagatgca ggcagctgag
      301 ttgttgtatt ctgataagag tcagaggtaa ctcccgttgc ggtgctgtta acggtggagg
      361 gcagtgtagt ctgagcagta ctcgttgctg ccgcgcgcgc caccagacat aatagctgac
      421 agactaacag actgttcctt tccatgggtc ttttctgcag aagcttgaat tctcgaggat
      481 ccgcggccgc gccaccatgt ggctggtaac tttcctcctg ctcctggact ctttacacaa
      541 agcccgcccc gaggatgtcg gcaccagcct ctactttgta aatgactccc tgcagcacgt
      601 gaccttctcc agttctgtgg gtgtggtggt gccctgcccg gcagctggat cccccagtgc
      661 ggctctccga tggtacctgg ccacggggga cgacatctac gatgtgccac acatccggca
      721 cgtgcatgcc aacgggactc tgcagctctt ccctttctcc ccctccgcct tcaatagctt
      781 tatccacgac aatgactatt tctgcactgc agagaacgca gccggcaaga tccggagccc
      841 taacattcgc atcaaagcag ttttcaggga accctacacc gtccgggtgg aggatcaaag
      901 gtcaatgcgt ggcaacgtgg ctgtctttaa gtgcctcatc ccctcttcag tgcaggaata
      961 cgttagcgtc gtgtcttggg agaaagacac ggtctccatc actccagaaa acaggttttt
     1021 catcacatca catggtggac tgtacatctc ggacgtgcag aaggaagatg ctctctccac
     1081 ctaccgctgc atcacccagc acaaatacag tggggagact cgtcagagca acggagctcg
     1141 gctctctgtg acagaccctg cagagtccat ccctaccatc ctggatggct tccactccca
     1201 ggaagtgtgg actggccact cggtagagct gccctgtgct gcttccggct accccatccc
     1261 tgccatccgc tggctcaaag atggcagacc cctccctgct gacagccgct gggctaagcg
     1321 cattacaggg ctgaccatca gtgacctgcg cactgaggac agcggcacct acatctgtga
     1381 ggtcaccaac acctttgggt cagcagaggc caacggcatc ctcacagtca ttgaccctct
     1441 tcatgtgacc ctgacaccaa agaagctgaa gacgggcatc gggagcacgg tcatcctatc
     1501 ctgcgccctc acaggctccc cagagttcac catccgttgg taccgcaaca cagaactggt
     1561 gctgccgggt gaggccatct ccatccgtgg gctcagcaat gagactctgc tcatctcctc
     1621 ggcacagaag agccactcgg gagcctacca gtgcttcgcc acccgcaagg cccagacagc
     1681 ccaggacttt gccatcattg tgctggaaga cggcacgccc cgcatcgtct catccttcag
     1741 cgagaaggtg gtcaaccctg gggagcagtt ctcactgatg tgtgccgcca agggcgcccc
     1801 gccccccacg gtcacctggg ccctggacga cgagcccgtt gtgcgggacg gaagccaccg
     1861 caccaaccag tacaccatgt ccgacggcac caccatcagc cacatgaacg tcacgggtcc
     1921 ccagatccgg gatgggggcg tgtaccggtg cacagcgcgg aactcggtgg gcagtgctga
     1981 atatcaggcg cgaataaacg taagaggacc ccccagcatc cgggcaatga ggaacatcac
     2041 ggcagtcgcc gggcgggaca cactcatcaa ctgcagggtc attggctacc cctactactc
     2101 catcaagtgg tacaaggatg ccctgctgct gcccgataac caccgccagg tggtgtttga
     2161 gaatgggact ctcaagctga cggatgtgca gaagggcatg gatgaggggg aatacctgtg
     2221 cagtgttctc atccagccac agctttccat cagccagagt gtccacgtgg ccgtcaaagt
     2281 gccccctctg atccagccct tcgagttccc ccctgcctcc atcggccagc tgctctacat
     2341 tccctgtgtg gtgtcctcgg gggacatgcc aatccgcatc acctggagga aggacggaca
     2401 ggtgatcatc tcaggctcag gcgtgaccat cgagagcaag gagttcatga gctccctgca
     2461 gatctccagc gtctccctca agcacaacgg caactacact tgcatcgcca gcaacgcagc
     2521 ggccactgtg agcagggagc gccagctcat cgtgcgtgtg ccccctcgat ttgtggtgca
     2581 gcccaataac caagatggca tctacggcaa agctggtgtg ctcaactgct cggtagatgg
     2641 ctacccacca cccaaggtga tgtggaagca tgccaaaggg agtgggaacc cccagcaata
     2701 tcacccggtg cccctcactg gccgcatcca gatcctaccc aacagctccc tgctgatacg
     2761 ccacgtcctg gaagaggaca tcggctacta cctctgccag gccagcaacg gcgtgggcac
     2821 cgacatcagc aaggccatgt tcctcaccgt gaaaatccca gcaatgatca catctcaccc
     2881 caacaccacg atcgccatca agggccaccc caaggagctg aactgcactg cgcgtggcga
     2941 gcggcccatc ataatccgct gggagaaggg ggacacagtc attgatcccg accgtgtcat
     3001 gcgctatgcc attgctacca aggacaatgg cgatgaagtg gtctccacgc ttaagctcaa
     3061 gccagctgac cgtggggact ccgtgttctt cagctgccat gctatcaact catatggaga
     3121 ggaccgaggt ttgatccagc tcactgtgca agagccccct gaccccccag agctagagat
     3181 ccgggaggtg aaggcccgga gcatgaacct gcgctggact cagagatttg atggaaacag
     3241 catcatcacc ggctttgata ttgaatacaa gaacaaatca gattcctggg attttaagca
     3301 gtccacacgc aacatctccc caactatcaa ccaggcgaac atcgtggact tacaccctgc
     3361 atcggtgtac agcatccgca tgtactcctt caacaagatc gggcgcagcg agccaagcaa
     3421 ggagcttacc atcagtacgg aagaggctgc tcctgatggg cctcccatgg atgtcacctt
     3481 gcaaccagtg acttcacaga gcatccaggt gacctggaag gcaccaaaga aggagctgca
     3541 gaatggagtc atcaggggat accagattgg ctaccgggag aacagccctg gcagtaatgg
     3601 gcagtacagc attgtggaaa tgaaggcgac gggggacagt gaggtctaca ccctggataa
     3661 cctcaagaag ttcgctcagt acggcgtggt ggtccaagcc ttcaaccggg caggcacggg
     3721 gccctcatct agtgagatca atgccaccac cttggaggat gtgcccagcc agcctccaga
     3781 gaatgtccga gctctgtcca tcacttctga tgtggctgtc atctcctggt cggagccccc
     3841 acgaagtacc ctcaatggtg tcctcaaagg ctaccgggtc atcttctggt cactctacgt
     3901 tgatggagag tggggcgaga tgcagaatgt caccaccaca agggagcgag tggagcttcg
     3961 cggtatggag aagttcacca actacagcgt ccaggtgctc gcctacacgc aggctgggga
     4021 tggtgtgcgc agcagcgtgc tctacatcca gaccaaggaa gatgttccag gcccccctgc
     4081 tggcatcaaa gctgtccctt catcagctag cagtgtggtc gtgtcctggc tgcccccaac
     4141 caagcccaat ggggtgatcc gcaaatacac catcttctgt tccagccctg ggtccggtca
     4201 gccggctccc agcgagtacg agaccagtcc tgaacagctc ttctaccgga tcgcccacct
     4261 gaaccgggga cagcagtact tgctatgggt ggctgcggtc acctctgcag gcagggggaa
     4321 cagcagcgag aaggtcacca ttgaacctgc tggcaaggcc ccagcaaaga tcatctcatt
     4381 tggaggcact gtaacaacac cctggatgaa agatgtccgg ctgccttgca attcagtagg
     4441 agatccagcc ccggctgtga agtggaccaa ggacagtgaa gactcagcaa tccccgtgtc
     4501 cttggatgga caccggctta tccacacaaa cggcacactg ctgcttcgtg ctgtgaaggc
     4561 tgaagactcc ggctattata cctgcacagc caccaacact ggtggctttg ataccatcat
     4621 cgtcaacctt ctggtacaag ttcccccgga ccagccccgc ctcactgtct ccaaaacctc
     4681 tgcctcttct atcaccctga cctggattcc aggagacaat gggggcagct ccatcagagg
     4741 cttcgtgttg caatactctg tggacaacag cgaggagtgg aaggatgtgt tcatcagctc
     4801 tagcgagcgc tccttcaagc tagacagcct caagtgtggc acgtggtaca aggtgaagct
     4861 ggcggccaag aacagtgtgg gctctgggcg catcagcgag atcatcgagg ccaagaccca
     4921 tgggcgggag ccttccttca gcaaagacca acacctcttc acccacatca actccacaca
     4981 tgctcggctt aacctgcagg gctggaacaa tggaggttgt cccatcacag ccattgtcct
     5041 ggagtaccgg cccaagggga cctgggcctg gcagggcgtc cgagccaaca gctccacaga
     5101 ggtgtttctg acagaacttc gagaggctac gtggtatgag ctacgcatga gggcttgcaa
     5161 cagcgccggc tgtggcaacg agaccgccca gtttgccacc ctggactacg acggcagcac
     5221 catcccaccc atcaaatctg ctcaaggaga aggcgatgac gtgaagaagg gcgcgcctgg
     5281 atcttccgga atcatcccag ttgaggagga gaacccggac ttctggaacc gcgaggcagc
     5341 cgaggccctg ggtgccgcca agaagctgca gcctgcacag acagccgcca agaacctcat
     5401 catcttcctg ggcgatggga tgggggtgtc tacggtgaca gctgccagga tcctaaaagg
     5461 gcagaagaag gacaaactgg ggcctgagat acccctggcc atggaccgct tcccatatgt
     5521 ggctctgtcc aagacataca atgtagacaa acatgtgcca gacagtggag ccacagccac
     5581 ggcctacctg tgcggggtca agggcaactt ccagaccatt ggcttgagtg cagccgcccg
     5641 ctttaaccag tgcaacacga cacgcggcaa cgaggtcatc tccgtgatga atcgggccaa
     5701 gaaagcaggg aagtcagtgg gagtggtaac caccacacga gtgcagcacg cctcgccagc
     5761 cggcacctac gcccacacgg tgaaccgcaa ctggtactcg gacgccgacg tgcctgcctc
     5821 ggcccgccag gaggggtgcc aggacatcgc tacgcagctc atctccaaca tggacattga
     5881 cgtgatccta ggtggaggcc gaaagtacat gtttcgcatg ggaaccccag accctgagta
     5941 cccagatgac tacagccaag gtgggaccag gctggacggg aagaatctgg tgcaggaatg
     6001 gctggcgaag cgccagggtg cccggtatgt gtggaaccgc actgagctca tgcaggcttc
     6061 cctggacccg tctgtgaccc atctcatggg tctctttgag cctggagaca tgaaatacga
     6121 gatccaccga gactccacac tggacccctc cctgatggag atgacagagg ctgccctgcg
     6181 cctgctgagc aggaaccccc gcggcttctt cctcttcgtg gagggtggtc gcatcgacca
     6241 tggtcatcat gaaagcaggg cttaccgggc actgactgag acgatcatgt tcgacgacgc
     6301 cattgagagg gcgggccagc tcaccagcga ggaggacacg ctgagcctcg tcactgccga
     6361 ccactcccac gtcttctcct tcggaggcta ccccctgcga gggagctcca tcttcgggct
     6421 ggcccctggc aaggcccggg acaggaaggc ctacacggtc ctcctatacg gaaacggtcc
     6481 aggctatgtg ctcaaggacg gcgcccggcc ggatgttacc gagagcgaga gcgggagccc
     6541 cgagtatcgg cagcagtcag cagtgcccct ggacgaagag acccacgcag gcgaggacgt
     6601 ggcggtgttc gcgcgcggcc cgcaggcgca cctggttcac ggcgtgcagg agcagacctt
     6661 catagcgcac gtcatggcct tcgccgcctg cctggagccc tacaccgcct gcgacctggc
     6721 gccccccgcc ggcaccaccg acgccgcgca cccgggtcat catcaccatc accattaatc
     6781 tagagatctt attaaagcag aacttgttta ttgcagctta taatggttac aaataaagca
     6841 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     6901 ccaaactcat caatgtatct tatcatgtct ggtcgactct agctagactc ttccgcttcc
     6961 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     7021 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     7081 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     7141 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     7201 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     7261 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     7321 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     7381 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     7441 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     7501 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     7561 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     7621 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     7681 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     7741 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     7801 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     7861 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     7921 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     7981 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     8041 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     8101 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     8161 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     8221 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     8281 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     8341 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     8401 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     8461 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     8521 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     8581 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     8641 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     8701 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     8761 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     8821 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     8881 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     8941 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     9001 gaggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccaggca ggcagaagta
     9061 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     9121 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     9181 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     9241 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
     9301 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
     9361 ccattttcgt cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     9421 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat
     9481 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
     9541 ggagaaaata ccgcatcagg aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
     9601 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     9661 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     9721 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     9781 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     9841 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     9901 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     9961 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccat
    10021 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
    10081 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
    10141 tcccagtcac gacgttgtaa aacgacggcc agtgaattgt cgaggtctcg acggtatcga
    10201 ttgacattga ttattgacta gctagttatt aatagtaatc aattacgggg tcattagttc
    10261 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
    10321 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
    10381 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
    10441 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
    10501 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
    10561 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
    10621 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
    10681 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
    10741 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
    10801 accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg
    10861 accgatccag cctccgcggc cgggaacggt gcattggaac gcggattccc cgtgccaaga
    10921 gtgacgtaag taccgcctat agactctata ggcacacccc tttggctctt atgcatgcta
    10981 tactgttttt ggcttggggc ctatacaccc ccgcttccat atgctatagg tgatggtata
    11041 gcttagccta taggtgtggg ttattgacca ttattgacca ctcccctatt ggtgacgata
    11101 ctttccatta ctaatccata acatggctct ttgccacaac tatctctatt ggctatatgc
    11161 caatactctg tccttcagag actgacacgg actctgtatt tttacaggat ggggtcccat
    11221 ttattattta caaattcaca tatacaacaa cgccgtcccc cgtgcccgca gtttttatta
    11281 aacatagcgt g
//
