LOCUS       sequence-131135-        8054 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the extracellular region of the FLRT3 protein, 
            C-terminally fused to alkaline phosphatase + 6X histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8054)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 8054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Flrt3-AP-His
FEATURES             Location/Qualifiers
     source          1..8054
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     CDS             4634..4651
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4677..4798
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4714..4733)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4768..4787
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4836..4853)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5007..5595)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5087..5106)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5766..6557,6558..6626))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6389..6408
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6627..6731)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6799..6817
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6855..6877)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6879..6899)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6884..7214
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7065..7200
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7127..7146
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     7351..7370
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(7437..7892)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7574..7595)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7786..7805
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8018..8040
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8032..8049
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8033..8049
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tgatcagccc
     1621 agcctggagc ctcttcctca tcgggactaa aattgggctg ttcttccaag tggcacctct
     1681 gtcagttgtg gctaaatcct gtccatctgt atgtcgctgt gacgcaggct tcatttactg
     1741 taacgatcgc tctctgacat ccattccagt gggaattccg gaggatgcta caacactcta
     1801 ccttcagaac aaccaaataa acaatgttgg gattccttcc gatttgaaga acttgctgaa
     1861 agtacaaaga atatacctat accacaacag tttagatgaa ttccctacca accttccaaa
     1921 gtatgtcaaa gagttacatt tgcaagagaa taacataagg actatcacct atgattcact
     1981 ttcgaaaatt ccgtatctgg aagagttaca cttggatgat aactcagtct cggctgttag
     2041 catcgaagag ggagcatttc gagacagtaa ctatctgcgg ctgctttttc tgtcccgtaa
     2101 ccaccttagc acaatcccgg ggggcttgcc caggactatt gaggaattac gcctggatga
     2161 caatcgcata tcaacgatct cttccccatc acttcatggt ctcacaagcc tgaaacgcct
     2221 ggttttagat ggaaacttgt tgaacaacca tggtttgggt gacaaagttt tcttcaactt
     2281 agtaaactta acagagctgt ccctggtgag gaattccttg acagcagcgc cagtgaacct
     2341 tcccggcaca agcctgagga agctttacct tcaagacaac catatcaacc gggtaccccc
     2401 aaatgctttt tcttatttaa ggcagctgta tcgactcgat atgtctaata ataacctaag
     2461 caatttacct cagggtatct ttgatgattt ggacaatata acccaactga ttcttcgcaa
     2521 caatccttgg tattgtggat gcaagatgaa atgggtacga gactggttac agtcgctacc
     2581 ggtgaaggtc aatgtgcgtg ggctcatgtg ccaagcccca gaaaaggtcc gtggaatggc
     2641 tatcaaggac ctcagtgcag aactgtttga ttgtaaagac agtgggattg tgagcaccat
     2701 tcagataacc actgcaatac ccaacacagc atatcctgct caaggacagt ggccagctcc
     2761 tgtgaccaaa caaccagata ttaaaaaccc caagctcatt aaggatcagc gaactacagg
     2821 cagcccctca cggaaaacaa ttttaattac tgtgaaatct gtcacccctg acacaatcca
     2881 catatcctgg agacttgctc tgcctatgac tgctctgcga ctcagctggc ttaaactggg
     2941 ccatagccca gcctttggat ctataacaga aacaatcgta acaggagaac gcagtgaata
     3001 cttggtcacc gccctagaac ctgaatcacc ctatagagta tgcatggttc ccatggaaac
     3061 cagtaacctt tacctgtttg atgaaacacc tgtttgtatt gagacccaaa ctgcccctct
     3121 tcgaatgtac aaccccacaa ccaccctcac tagtggatct tccggaatca tcccagttga
     3181 ggaggagaac ccggacttct ggaaccgcga ggcagccgag gccctgggtg ccgccaagaa
     3241 gctgcagcct gcacagacag ccgccaagaa cctcatcatc ttcctgggcg atgggatggg
     3301 ggtgtctacg gtgacagctg ccaggatcct aaaagggcag aagaaggaca aactggggcc
     3361 tgagataccc ctggccatgg accgcttccc atatgtggct ctgtccaaga catacaatgt
     3421 agacaaacat gtgccagaca gtggagccac agccacggcc tacctgtgcg gggtcaaggg
     3481 caacttccag accattggct tgagtgcagc cgcccgcttt aaccagtgca acacgacacg
     3541 cggcaacgag gtcatctccg tgatgaatcg ggccaagaaa gcagggaagt cagtgggagt
     3601 ggtaaccacc acacgagtgc agcacgcctc gccagccggc acctacgccc acacggtgaa
     3661 ccgcaactgg tactcggacg ccgacgtgcc tgcctcggcc cgccaggagg ggtgccagga
     3721 catcgctacg cagctcatct ccaacatgga cattgacgtg atcctaggtg gaggccgaaa
     3781 gtacatgttt cgcatgggaa ccccagaccc tgagtaccca gatgactaca gccaaggtgg
     3841 gaccaggctg gacgggaaga atctggtgca ggaatggctg gcgaagcgcc agggtgcccg
     3901 gtatgtgtgg aaccgcactg agctcatgca ggcttccctg gacccgtctg tgacccatct
     3961 catgggtctc tttgagcctg gagacatgaa atacgagatc caccgagact ccacactgga
     4021 cccctccctg atggagatga cagaggctgc cctgcgcctg ctgagcagga acccccgcgg
     4081 cttcttcctc ttcgtggagg gtggtcgcat cgaccatggt catcatgaaa gcagggctta
     4141 ccgggcactg actgagacga tcatgttcga cgacgccatt gagagggcgg gccagctcac
     4201 cagcgaggag gacacgctga gcctcgtcac tgccgaccac tcccacgtct tctccttcgg
     4261 aggctacccc ctgcgaggga gctccatctt cgggctggcc cctggcaagg cccgggacag
     4321 gaaggcctac acggtcctcc tatacggaaa cggtccaggc tatgtgctca aggacggcgc
     4381 ccggccggat gttaccgaga gcgagagcgg gagccccgag tatcggcagc agtcagcagt
     4441 gcccctggac gaagagaccc acgcaggcga ggacgtggcg gtgttcgcgc gcggcccgca
     4501 ggcgcacctg gttcacggcg tgcaggagca gaccttcata gcgcacgtca tggccttcgc
     4561 cgcctgcctg gagccctaca ccgcctgcga cctggcgccc cccgccggca ccaccgacgc
     4621 cgcgcacccg ggtcatcatc accatcacca ttaatctaga gatcttatta aagcagaact
     4681 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     4741 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     4801 atgtctggtc gactctagct agactcttcc gcttcctcgc tcactgactc gctgcgctcg
     4861 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     4921 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     4981 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     5041 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     5101 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     5161 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     5221 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     5281 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     5341 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     5401 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     5461 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     5521 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     5581 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     5641 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     5701 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     5761 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     5821 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     5881 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     5941 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     6001 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     6061 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     6121 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     6181 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     6241 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     6301 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     6361 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     6421 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     6481 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     6541 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     6601 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     6661 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     6721 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     6781 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
     6841 gatgacggtg aaaacctctg acacatgcag ctcccggagg aatgtgtgtc agttagggtg
     6901 tggaaagtcc ccaggctccc caggcaggca gaagtatgca aagcatgcat ctcaattagt
     6961 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     7021 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     7081 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     7141 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     7201 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcgtcccg gagacggtca
     7261 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     7321 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     7381 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggaaat
     7441 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     7501 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     7561 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     7621 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     7681 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     7741 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     7801 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     7861 cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc cattcaggct gcgcaactgt
     7921 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     7981 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     8041 acggccagtg aatt
//
