LOCUS       sequence-13114-e        3366 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 3366)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1500-HQ858758
FEATURES             Location/Qualifiers
     source          1..3366
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             112..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(923..1022)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1039..1058)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1040..1058)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1063..1079)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1063..1079)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1181..1200)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1192..2001
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1259..1278)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2148..2736
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2637..2656
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2890..2907
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3066..3093)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3185..3271)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3320..3342
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3334..3351
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3335..3351
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc cttgtttaac
      121 tttaagaagg agcccttcac catgaacggc gggggctctg ggctggcgcc caacgccgcg
      181 cacaccgcgg acgaggtctt ccgcgactac aaaggccgcc gcgccggcat gatcaaggcc
      241 ctcaccactg atgtggagag gttcttcaaa ctctgtgacc ctgaaaagga gaacttgtgc
      301 ctttatggct atcccgatga gacgtgggag gtgaccttgc cagctgagga agttccccct
      361 gagatccctg aaccagcatt aggaatcaac tttgctaggg atggcatgaa tgagaaagac
      421 tggctggcgc tagttgcagt ccacagtgat tcctggttac tgtctgttgc attctacttt
      481 ggtgcacgct ttggatttga cagagaaact aggaggcgac tctttagcct gataaacaac
      541 atgcccacga tatttgaggt agtgacagga gcagccaaga agcaggcgaa ggagaagaca
      601 cctaatagta gcagcaagag caacaggcct agttcaaaag tgtcaagagc agagtctcgt
      661 tcaaaggcta aggtccccca agacgaagaa gagagcggtg atgacgatga ggatgaggag
      721 gcagacgagc acaacaatac cttgtgtgga acttgcggaa ctaacgacag caaggaccag
      781 ttctggatct gttgtgataa ctgcgagaag tggtaccatg ggaagtgtgt caagatcacg
      841 ccagctcgag ctgagcatat caagcagtac aagtgcccag actgcaccaa caagcgggcc
      901 agggcaaagg gtgggcgcgc cgacccagct ttcttgtaca aagttggcat tataagaaag
      961 cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt
     1021 tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg
     1081 cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca
     1141 tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat
     1201 attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg
     1261 tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg
     1321 aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt
     1381 acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag
     1441 cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca
     1501 gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca
     1561 gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc
     1621 gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat
     1681 tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt
     1741 ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt
     1801 tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga
     1861 taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa
     1921 cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg
     1981 atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt
     2041 acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt gagttacgcg
     2101 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     2161 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     2221 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     2281 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     2341 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     2401 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     2461 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     2521 agatacctac agcgtgagca ttgagaaagc gccacgcttc ccgaagggag aaaggcggac
     2581 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     2641 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     2701 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     2761 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     2821 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     2881 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     2941 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     3001 gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa
     3061 acgcaaaaag gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg
     3121 gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg
     3181 gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc
     3241 agtcttccga ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcgtta
     3301 acgctagcat ggatgttttc ccagtcacga cgttgtaaaa cgacggccag tcttaagctc
     3361 gggccc
//
