LOCUS       sequence-131145-        8093 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the extracellular region of the LRRC4C protein, 
            C-terminally fused to alkaline phosphatase + 6X histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8093)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 8093)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Lrrc4c-AP-His
FEATURES             Location/Qualifiers
     source          1..8093
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3570..3587
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3613..3734
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3650..3669)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3704..3723
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3772..3789)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3943..4531)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4023..4042)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4702..5493,5494..5562))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5325..5344
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5563..5667)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5735..5753
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5791..5813)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5815..5835)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5820..6150
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6001..6136
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6063..6082
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     6287..6306
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(6373..6828)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6510..6531)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6722..6741
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6954..6976
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6968..6985
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6969..6985
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        7015..7398
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7399..7602
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7553..7573
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     7599..7623
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          join(7738..8093,1..470)
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
ORIGIN
        1 acatagcgtg ggatctccac gcgaatctcg ggtacgtgtt ccggacatgg gctcttctcc
       61 ggtagcggcg gagcttccac atccgagccc tggtcccatg cctccagcgg ctcatggtcg
      121 ctcggcagct ccttgctcct aacagtggag gccagactta ggcacagcac aatgcccacc
      181 accaccagtg tgccgcacaa ggccgtggcg gtagggtatg tgtctgaaaa tgagctcgga
      241 gattgggctc gcaccgctga cgcagatgga agacttaagg cagcggcaga agaagatgca
      301 ggcagctgag ttgttgtatt ctgataagag tcagaggtaa ctcccgttgc ggtgctgtta
      361 acggtggagg gcagtgtagt ctgagcagta ctcgttgctg ccgcgcgcgc caccagacat
      421 aatagctgac agactaacag actgttcctt tccatgggtc ttttctgcag aagcttgaat
      481 tctcgaggat ccgcggccgc gccaccatgt tgaacaagat gaccttacat ccacagcaga
      541 taatgatagg tcctaggttt aacagggccc tatttgaccc cctgcttgtg gtgctgttgg
      601 ctcttcaact tcttgtggtg gctggtttgg ttcgtgctca aacctgccct tcagtgtgct
      661 cttgtagcaa ccagttcagc aaggtgattt gtgttcggaa aaaccttcgt gaagttccgg
      721 atggcatctc caccaacaca aggctgctga acctccatga gaaccaaatc cagatcatta
      781 aagtgaacag cttcaagcat ttgaggcact tagaaatcct ccagttgagc aggaaccata
      841 ttcgaaccat tgaaatcggg gccttcaatg gtctggccaa cctcaacacc ctggaactct
      901 ttgacaatcg tcttactacc ataccgaatg gagcttttgt atatttgtct aaactgaagg
      961 agctctggtt gaggaacaac cctattgaaa gcatcccttc ctatgctttt aacagaatcc
     1021 cttctttgcg ccgcctggac ttaggggaat tgaaaaggct ttcatacatc tcagaaggtg
     1081 cctttgaagg tctgtccaac ttgaggtatt tgaaccttgc catgtgcaac cttcgggaaa
     1141 tccctaacct cacaccactc atcaaacttg acgagctaga tctttctggg aaccatttgt
     1201 ctgcaatcag gcctggctct tttcaggggt tgatgcacct tcaaaaactg tggatgatac
     1261 agtctcagat tcaagtgatt gaacggaatg cctttgataa ccttcagtca ctagtggaga
     1321 taaacttggc acacaacaat ctaacattat tgcctcatga cctcttcaca cccttgcatc
     1381 atctagagag gatacacctc catcacaacc cgtggaactg taactgtgat atcctgtggc
     1441 tcagctggtg gataagagac atggccccct ccaacacagc ttgctgtgcc aggtgtaaca
     1501 ctccccccaa cctgaaaggg aggtacatcg gagagctgga ccagaattac tttacatgct
     1561 atgctcccgt aattgtggag ccccctgcag acctcaatgt cactgaaggc atggcagctg
     1621 agctgaaatg tcgggcttct acgtccctga cttccgtatc ttggattact ccaaatggaa
     1681 cagtcatgac ccacggggca tacaaagtgc ggatagctgt gctcagcgac ggtactttaa
     1741 atttcacaaa tgtaactgtg caagacacag gcatgtacac atgtatggtg agtaattctg
     1801 ttggcaacac cactgcttct gccaccttga atgttactgc ggcaaccact actccgttct
     1861 cgtacttttc aactgtaaca gtagagacta tggaaccttc tcaggatgag gcacggacca
     1921 cagataacaa tgtgggcccc actccagtga tcgattggga gaccaccaat gtaaccacat
     1981 ctcttacgcc acagagcaca aggtcgacag aaaaaacatt caccatccca gtaactgaca
     2041 tcaacagcgg aatcccagga attgatgagg tcatgaaaac aggcgcgcct ggatcttccg
     2101 gaatcatccc agttgaggag gagaacccgg acttctggaa ccgcgaggca gccgaggccc
     2161 tgggtgccgc caagaagctg cagcctgcac agacagccgc caagaacctc atcatcttcc
     2221 tgggcgatgg gatgggggtg tctacggtga cagctgccag gatcctaaaa gggcagaaga
     2281 aggacaaact ggggcctgag atacccctgg ccatggaccg cttcccatat gtggctctgt
     2341 ccaagacata caatgtagac aaacatgtgc cagacagtgg agccacagcc acggcctacc
     2401 tgtgcggggt caagggcaac ttccagacca ttggcttgag tgcagccgcc cgctttaacc
     2461 agtgcaacac gacacgcggc aacgaggtca tctccgtgat gaatcgggcc aagaaagcag
     2521 ggaagtcagt gggagtggta accaccacac gagtgcagca cgcctcgcca gccggcacct
     2581 acgcccacac ggtgaaccgc aactggtact cggacgccga cgtgcctgcc tcggcccgcc
     2641 aggaggggtg ccaggacatc gctacgcagc tcatctccaa catggacatt gacgtgatcc
     2701 taggtggagg ccgaaagtac atgtttcgca tgggaacccc agaccctgag tacccagatg
     2761 actacagcca aggtgggacc aggctggacg ggaagaatct ggtgcaggaa tggctggcga
     2821 agcgccaggg tgcccggtat gtgtggaacc gcactgagct catgcaggct tccctggacc
     2881 cgtctgtgac ccatctcatg ggtctctttg agcctggaga catgaaatac gagatccacc
     2941 gagactccac actggacccc tccctgatgg agatgacaga ggctgccctg cgcctgctga
     3001 gcaggaaccc ccgcggcttc ttcctcttcg tggagggtgg tcgcatcgac catggtcatc
     3061 atgaaagcag ggcttaccgg gcactgactg agacgatcat gttcgacgac gccattgaga
     3121 gggcgggcca gctcaccagc gaggaggaca cgctgagcct cgtcactgcc gaccactccc
     3181 acgtcttctc cttcggaggc taccccctgc gagggagctc catcttcggg ctggcccctg
     3241 gcaaggcccg ggacaggaag gcctacacgg tcctcctata cggaaacggt ccaggctatg
     3301 tgctcaagga cggcgcccgg ccggatgtta ccgagagcga gagcgggagc cccgagtatc
     3361 ggcagcagtc agcagtgccc ctggacgaag agacccacgc aggcgaggac gtggcggtgt
     3421 tcgcgcgcgg cccgcaggcg cacctggttc acggcgtgca ggagcagacc ttcatagcgc
     3481 acgtcatggc cttcgccgcc tgcctggagc cctacaccgc ctgcgacctg gcgccccccg
     3541 ccggcaccac cgacgccgcg cacccgggtc atcatcacca tcaccattaa tctagagatc
     3601 ttattaaagc agaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     3661 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     3721 atcaatgtat cttatcatgt ctggtcgact ctagctagac tcttccgctt cctcgctcac
     3781 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     3841 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     3901 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     3961 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     4021 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     4081 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     4141 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     4201 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     4261 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     4321 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     4381 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     4441 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     4501 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     4561 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     4621 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     4681 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     4741 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     4801 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     4861 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     4921 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     4981 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     5041 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     5101 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     5161 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     5221 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     5281 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     5341 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     5401 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     5461 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     5521 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     5581 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     5641 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     5701 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     5761 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggaggaatg
     5821 tgtgtcagtt agggtgtgga aagtccccag gctccccagg caggcagaag tatgcaaagc
     5881 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     5941 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     6001 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     6061 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
     6121 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
     6181 gtcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     6241 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     6301 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     6361 taccgcatca ggaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
     6421 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
     6481 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
     6541 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
     6601 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
     6661 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
     6721 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
     6781 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc attcgccatt
     6841 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
     6901 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
     6961 acgacgttgt aaaacgacgg ccagtgaatt gtcgaggtct cgacggtatc gattgacatt
     7021 gattattgac tagctagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     7081 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     7141 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     7201 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     7261 gtgtatcata tgccaagtcc gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     7321 cattatgccc agtacatgac cttacgggac tttcctactt ggcagtacat ctacgtatta
     7381 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca ccaatgggcg tggatagcgg
     7441 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     7501 caccaaaatc aacgggactt tccaaaatgt cgtaataacc ccgccccgtt gacgcaaatg
     7561 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag
     7621 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc
     7681 agcctccgcg gccgggaacg gtgcattgga acgcggattc cccgtgccaa gagtgacgta
     7741 agtaccgcct atagactcta taggcacacc cctttggctc ttatgcatgc tatactgttt
     7801 ttggcttggg gcctatacac ccccgcttcc atatgctata ggtgatggta tagcttagcc
     7861 tataggtgtg ggttattgac cattattgac cactccccta ttggtgacga tactttccat
     7921 tactaatcca taacatggct ctttgccaca actatctcta ttggctatat gccaatactc
     7981 tgtccttcag agactgacac ggactctgta tttttacagg atggggtccc atttattatt
     8041 tacaaattca catatacaac aacgccgtcc cccgtgcccg cagtttttat taa
//
