LOCUS       sequence-13115-e        4659 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4659)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 4659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1501-HQ858759
FEATURES             Location/Qualifiers
     source          1..4659
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             112..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(2216..2315)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2332..2351)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2333..2351)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2356..2372)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2356..2372)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2474..2493)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2485..3294
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2552..2571)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3441..4029
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3930..3949
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4183..4200
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4359..4386)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4478..4564)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4613..4635
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4627..4644
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4628..4644
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc cttgtttaac
      121 tttaagaagg agcccttcac catggcgcaa ggagcaggac gggacccgga ggagctcttc
      181 gaggagctgt ggcgcgcctg cgcggggccg ctcgtggagt tgccccagac caacgaaagg
      241 gtcttctact tcctccaggg tcacctagaa cagctgcaag agccgacgga cccggcgctg
      301 ctggctgagc agatcaagat gttccaggtg cccaacaaga tcctctgcaa ggtcgtcaac
      361 gtcgagctca aggccgagac agaaacggac gagatgtacg cccagatcac tctgcagccg
      421 gaaccagatc aagtggatct accccaatta cctgaaccgc ccctgcagga gacgtcaagg
      481 cccgtggtgc actccttctg caagatcctc acaccatccg acaccagtac tcatggcggt
      541 ttctccgttc ttcggcgtca tgcaaacgaa tgcctcccag cattggatat gtcgatgccg
      601 accccgaccc aagagctcat cacaaaggac ctacatggat ctgaatggag gttcaagcac
      661 atatacaggg gccaaccccg tcggcacctt ctgactactg gatggagcac gtttgtcaca
      721 tcaaagaagc tgattgctgg tgacgcgttt gtctacctga ggagcgagac cggacagcag
      781 cgtgtgggag tgaggcgcct cgtgcagaag caaagcacaa tgccagcatc tgttatatca
      841 agccaaagca tgcatcttgg ggtgcttgca agtgcatcac acgctattag gactcattcc
      901 attttcctag tgtattatag accaaggtta agccaaagcc aatacatcgt caggctgaac
      961 aagtacctgg aatctagtaa aattggattc gatgtgggca tgaggtttaa gatgagtttt
     1021 gaaggggatg acgtccctat aaagaagttt tctggaacag ttgtcgataa aggagatctt
     1081 tctccacaat ggcaaggttc tgaatggaag acattgaagg tcaaatggga cgaagccaca
     1141 aacttaaatg gccctgagag ggtttcctcc tgggaaattg aaccattcga tgcatccgca
     1201 cctgccatca ccatgcccgt gcaaccatca atgaaaaaca aaaggcctag ggagacagct
     1261 gaaggcctgg atattcatgc actggaacct gctcaagaat tctggctctc tggaaggcct
     1321 gaacagcatg agaagacaag cgtaagctcc agcgaaccca aacatcaagt cgcttggaca
     1381 agtgaacgcg caggatacag tgccatgagc agttcaatct gtcagaattc tgcagtaact
     1441 gggagctggt tcaagggctt caattcctcc ggctcactcc cttccctgcc agagatctcc
     1501 cagaaactgt ttcaggtcac cagcaacgac gcgagggttc ctccttggcc tgggctctcc
     1561 gcttatcatg ctgacgagcc ctcttccaag ctgtcctgca acactgctct gtgcagctac
     1621 cagaccgagg aggtggctcc aagattttcc aatgccgtcg aagaggaaaa gaaggagccc
     1681 ggcatgttcc gtctgtttgg cgtcaatttg attaaccatg ctaggagtag tgctaccgct
     1741 gacaagacga gtgtgggagc aggggaaaca tcggcgcgag ctgctggttc ttttgaagac
     1801 tctgctcagc tatcaagagt gacgaaggat catacgcaca tggtgaacgg aagcccccga
     1861 gaaatccaaa gccaccagag ttgcagtgga aggagtcgta tcaaggttca aatgcatgga
     1921 aatgacgtgt gcagagctgt ggatttggga aatctggatg ggtacgagca gctgatgggt
     1981 gaggttggag agatgtttga gatcaaagac cttggctcca aagagaaaga ggaatggaag
     2041 gttactttca ttaacgacga gaatgagaca atggaagtcg gagctgttcc ttggcaggag
     2101 ttttgccaga tggtgaggaa gatcgtgatc cattccattg gagacagggg ccatatggag
     2161 gcttgccctt gcctggggca ggatgggaag agggactata agggtgggcg cgccgaccca
     2221 gctttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg ttgcaacgaa
     2281 caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgatat cccctatagt
     2341 gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc
     2401 tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac
     2461 ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga
     2521 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg
     2581 caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg
     2641 aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg
     2701 ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca
     2761 tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct
     2821 gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt
     2881 cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca
     2941 cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct
     3001 gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc
     3061 actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt
     3121 attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac
     3181 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat
     3241 aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa
     3301 ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga cggcgcaagc
     3361 tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta
     3421 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     3481 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     3541 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     3601 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     3661 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     3721 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     3781 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gcattgagaa
     3841 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     3901 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     3961 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     4021 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     4081 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     4141 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     4201 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     4261 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     4321 acgcgtaccg ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg
     4381 ccttctgctt agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg
     4441 gccgttgctt cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt
     4501 tcaccgacaa acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta
     4561 tttgatgcct ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca
     4621 cgacgttgta aaacgacggc cagtcttaag ctcgggccc
//
