LOCUS       sequence-131150-        9881 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the extracellular region of the Neogenin 1, isoform b 
            protein, C-terminally fused to alkaline phosphatase + 6X histidine 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9881)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 9881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Neo1.b-AP-His
FEATURES             Location/Qualifiers
     source          1..9881
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     regulatory      1604..1613
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1608..1617
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6461..6478
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    6504..6625
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6541..6560)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6595..6614
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6663..6680)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6834..7422)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6914..6933)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7593..8384,8385..8453))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8216..8235
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8454..8558)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8626..8644
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8682..8704)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8706..8726)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8711..9041
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8892..9027
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8954..8973
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     9178..9197
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(9264..9719)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9401..9422)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9613..9632
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9845..9867
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9859..9876
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9860..9876
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tggcggcgga
     1621 gcgcgaagcc gggcgactcc tctgcacctc ctcctcccgg cgctgctgtc cgccaccgcc
     1681 gctgctgctg ttgctgccgc tgctgctgct gctcggacgc ccggcgtccg gcgccgcggc
     1741 cacgaagagc ggctccccgc cgcagtccgc aggagccagt gttcgaacat tcactccgtt
     1801 ttattttctg gtggagccag tagacaccct ctcagttaga ggctcttctg ttatattaaa
     1861 ttgctcggca tattctgagc cctctccaaa cattgaatgg aagaaagatg ggactttttt
     1921 aaacttagaa tcagatgatc gacgccagct actcccagat ggatctttat tcatcagcaa
     1981 cgtggtgcat tccaaacaca ataagcctga cgaaggtttc tatcagtgtg tagccactgt
     2041 ggataatctt ggaaccattg tcagcagaac agccaagctc acagtagcag gtcttccaag
     2101 atttaccagc caaccagaac cttcttcagt ctatgttgga aacagtgcaa ttctgaattg
     2161 tgaagttaat gcagatttgg tcccatttgt taggtgggaa cagaatcgac agccccttct
     2221 tctagatgac aggattgtca aacttccaag tggaacactg gttatcagca atgctactga
     2281 aggagatggg ggactctacc gctgcattgt tgaaagtggt gggccaccaa agtttagtga
     2341 cgaagctgaa ttgaaagttc ttcaagatcc tgaggaaatt gtagacttgg tatttctgat
     2401 gcgaccatct tctatgatga aagtcactgg tcagagtgca gtgttgccat gtgttgtctc
     2461 agggcttcct gctccagttg ttagatggat gaaaaacgaa gaagtgcttg acacagaaag
     2521 ctctggcagg ttggtcttgc tagcaggagg ttgcttggag atcagtgatg tcactgagga
     2581 tgatgctggg acttattttt gcatagctga taatggaaat aagacagttg aagctcaggc
     2641 ggagcttact gtgcaagtgc cacctggatt cctgaaacaa cctgctaaca tatatgctca
     2701 cgaatccatg gacattgtat ttgaatgtga agtcactggg aagccaactc caactgtgaa
     2761 gtgggtcaag aatggggatg tggttatccc cagtgattac tttaaaattg taaaggaaca
     2821 taatcttcaa gttttgggtc tggtgaaatc agatgaaggg ttctatcaat gcattgctga
     2881 gaatgatgtt ggaaatgcac aagctggagc ccagctgata atccttgagc atgatgttgc
     2941 catcccaaca ttacctccca cttcactgac cagtgccact actgaccatc tagcaccagc
     3001 cacaacggga ccattacctt cagctcctcg agacgtcgtg gcctccctgg tctctactcg
     3061 cttcattaaa ttgacatggc gtacacctgc atcagaccct catggagaca atctcaccta
     3121 ctctgtgttc tacaccaagg aaggggttgc tagggagcgt gttgagaata ccagccagcc
     3181 aggagagatg caggtgacta ttcaaaactt gatgccagca actgtgtaca tcttcaaagt
     3241 tatggctcaa aataagcatg gctctggaga aagttcagct cctcttcgag tagagacaca
     3301 gcctgaggtt cagctccctg gcccagcacc taatatccgt gcttatgcaa cgtcacctac
     3361 ttctatcact gtcacctggg aaacaccgtt atctggcaat ggggaaattc aaaattacaa
     3421 attgtactac atggaaaaag gaactgataa agaacaggat attgatgttt caagtcactc
     3481 ctacaccatt aatggactga agaaatacac agaatacagt ttccgagtgg tggcctacaa
     3541 taaacatggt cctggagttt ctacacaaga tgttgctgtt cgaacattat cagatgttcc
     3601 cagtgctgct cctcagaatc tgtccttaga agtgagaaat tcaaagagta tagtgatcca
     3661 ctggcagccc ccttcctcaa ccacacaaaa tgggcagata actggctaca agattcgata
     3721 tcgaaaggcc tcccgaaaaa gtgatgtcac tgagaccttg gtaactggga cacagctgtc
     3781 tcagctgatt gaaggtcttg atcgggggac agaatataac ttccgagtcg ctgctctcac
     3841 agtcaatggt acaggtccag caactgattg gctgtctgct gaaacttttg aaagcgacct
     3901 agatgaaact cgtgttcctg aagtgcccag ctctcttcat gtccgtccgc tcgtcactag
     3961 cattgtagtg agctggactc ctccagagaa ccagaacatt gtggtccgag gttatgccat
     4021 cggttacggc attggcagcc ctcatgccca gaccatcaaa gtggactata aacaacgtta
     4081 ttacaccatc gaaaacttgg atccaagctc tcattacgtg attaccttga aagcatttaa
     4141 caatgttggc gaaggcatcc ccctttatga gagtgctgtg accagacctc acacagacac
     4201 ttctgaagtt gatttatttg ttattaatgc tccatacact ccagtgccag atcccactcc
     4261 catgatgcca ccagtgggag ttcaggcttc cattctgagt cacgacacca taaggattac
     4321 ctgggcagac aactccctgc ccaaacacca gaagattaca gactcccgct actacacagt
     4381 ccggtggaag accaacatcc cagcaaacac gaagtacaag aatgcaaatg caacgacgtt
     4441 aagctatttg gttactggtt taaagccaaa tacgctctat gagttctctg tgatggtgac
     4501 caaaggcaga aggtcaagca cgtggagtat gacagctcat ggcgctacct ttgaattagt
     4561 tcctacttct ccacctaagg atgtgacagt tgtgagtaag gaaggaaaac ctagaaccat
     4621 catagtgaac tggcagcctc cctctgaagc taacggcaag attacaggtt acatcatcta
     4681 ttacagcacg gatgtgaatg cagagataca tgactgggtt attgaaccag ttgtgggaaa
     4741 cagactgact caccagattc aagagttaac acttgatacg ccatactact tcaaaatcca
     4801 ggcccggaac tcaaagggca tggggcccat gtctgaagct gtacagttca gaacacctaa
     4861 agccttaggg tcagcaggaa aaggaagccg actaccagac ctgggatctg actacaaacc
     4921 tccaatgagt ggcagcaaca gccctcacgg gagccccacc tcccctctgg acagcactag
     4981 tggatcttcc ggaatcatcc cagttgagga ggagaacccg gacttctgga accgcgaggc
     5041 agccgaggcc ctgggtgccg ccaagaagct gcagcctgca cagacagccg ccaagaacct
     5101 catcatcttc ctgggcgatg ggatgggggt gtctacggtg acagctgcca ggatcctaaa
     5161 agggcagaag aaggacaaac tggggcctga gatacccctg gccatggacc gcttcccata
     5221 tgtggctctg tccaagacat acaatgtaga caaacatgtg ccagacagtg gagccacagc
     5281 cacggcctac ctgtgcgggg tcaagggcaa cttccagacc attggcttga gtgcagccgc
     5341 ccgctttaac cagtgcaaca cgacacgcgg caacgaggtc atctccgtga tgaatcgggc
     5401 caagaaagca gggaagtcag tgggagtggt aaccaccaca cgagtgcagc acgcctcgcc
     5461 agccggcacc tacgcccaca cggtgaaccg caactggtac tcggacgccg acgtgcctgc
     5521 ctcggcccgc caggaggggt gccaggacat cgctacgcag ctcatctcca acatggacat
     5581 tgacgtgatc ctaggtggag gccgaaagta catgtttcgc atgggaaccc cagaccctga
     5641 gtacccagat gactacagcc aaggtgggac caggctggac gggaagaatc tggtgcagga
     5701 atggctggcg aagcgccagg gtgcccggta tgtgtggaac cgcactgagc tcatgcaggc
     5761 ttccctggac ccgtctgtga cccatctcat gggtctcttt gagcctggag acatgaaata
     5821 cgagatccac cgagactcca cactggaccc ctccctgatg gagatgacag aggctgccct
     5881 gcgcctgctg agcaggaacc cccgcggctt cttcctcttc gtggagggtg gtcgcatcga
     5941 ccatggtcat catgaaagca gggcttaccg ggcactgact gagacgatca tgttcgacga
     6001 cgccattgag agggcgggcc agctcaccag cgaggaggac acgctgagcc tcgtcactgc
     6061 cgaccactcc cacgtcttct ccttcggagg ctaccccctg cgagggagct ccatcttcgg
     6121 gctggcccct ggcaaggccc gggacaggaa ggcctacacg gtcctcctat acggaaacgg
     6181 tccaggctat gtgctcaagg acggcgcccg gccggatgtt accgagagcg agagcgggag
     6241 ccccgagtat cggcagcagt cagcagtgcc cctggacgaa gagacccacg caggcgagga
     6301 cgtggcggtg ttcgcgcgcg gcccgcaggc gcacctggtt cacggcgtgc aggagcagac
     6361 cttcatagcg cacgtcatgg ccttcgccgc ctgcctggag ccctacaccg cctgcgacct
     6421 ggcgcccccc gccggcacca ccgacgccgc gcacccgggt catcatcacc atcaccatta
     6481 atctagagat cttattaaag cagaacttgt ttattgcagc ttataatggt tacaaataaa
     6541 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     6601 tgtccaaact catcaatgta tcttatcatg tctggtcgac tctagctaga ctcttccgct
     6661 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     6721 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     6781 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     6841 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     6901 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     6961 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     7021 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     7081 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     7141 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     7201 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     7261 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     7321 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     7381 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     7441 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     7501 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     7561 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     7621 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     7681 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     7741 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     7801 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     7861 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     7921 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     7981 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     8041 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     8101 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     8161 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     8221 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     8281 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     8341 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     8401 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     8461 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     8521 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     8581 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     8641 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     8701 ccggaggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag gcaggcagaa
     8761 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     8821 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     8881 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     8941 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
     9001 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
     9061 tatccatttt cgtcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     9121 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
     9181 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     9241 taaggagaaa ataccgcatc aggaaattgt aagcgttaat attttgttaa aattcgcgtt
     9301 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     9361 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     9421 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     9481 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     9541 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     9601 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     9661 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     9721 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     9781 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     9841 ttttcccagt cacgacgttg taaaacgacg gccagtgaat t
//
