LOCUS       sequence-131152-        9854 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the extracellular region of the Neogenin 1, isoform d 
            protein, C-terminally fused to alkaline phosphatase + 6X histidine 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9854)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 9854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Neo1.d-AP-His
FEATURES             Location/Qualifiers
     source          1..9854
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     regulatory      1604..1613
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1608..1617
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6434..6451
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    6477..6598
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6514..6533)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6568..6587
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6636..6653)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6807..7395)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6887..6906)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7566..8357,8358..8426))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8189..8208
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8427..8531)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8599..8617
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8655..8677)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8679..8699)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8684..9014
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8865..9000
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8927..8946
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     9151..9170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(9237..9692)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9374..9395)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9586..9605
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9818..9840
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9832..9849
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9833..9849
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tggcggcgga
     1621 gcgcgaagcc gggcgactcc tctgcacctc ctcctcccgg cgctgctgtc cgccaccgcc
     1681 gctgctgctg ttgctgccgc tgctgctgct gctcggacgc ccggcgtccg gcgccgcggc
     1741 cacgaagagc ggctccccgc cgcagtccgc aggagccagt gttcgaacat tcactccgtt
     1801 ttattttctg gtggagccag tagacaccct ctcagttaga ggctcttctg ttatattaaa
     1861 ttgctcggca tattctgagc cctctccaaa cattgaatgg aagaaagatg ggactttttt
     1921 aaacttagaa tcagatgatc gacgccagct actcccagat ggatctttat tcatcagcaa
     1981 cgtggtgcat tccaaacaca ataagcctga cgaaggtttc tatcagtgtg tagccactgt
     2041 ggataatctt ggaaccattg tcagcagaac agccaagctc acagtagcag gtcttccaag
     2101 atttaccagc caaccagaac cttcttcagt ctatgttgga aacagtgcaa ttctgaattg
     2161 tgaagttaat gcagatttgg tcccatttgt taggtgggaa cagaatcgac agccccttct
     2221 tctagatgac aggattgtca aacttccaag tggaacactg gttatcagca atgctactga
     2281 aggagatggg ggactctacc gctgcattgt tgaaagtggt gggccaccaa agtttagtga
     2341 cgaagctgaa ttgaaagttc ttcaagatcc tgaggaaatt gtagacttgg tatttctgat
     2401 gcgaccatct tctatgatga aagtcactgg tcagagtgca gtgttgccat gtgttgtctc
     2461 agggcttcct gctccagttg ttagatggat gaaaaacgaa gaagtgcttg acacagaaag
     2521 ctctggcagg ttggtcttgc tagcaggagg ttgcttggag atcagtgatg tcactgagga
     2581 tgatgctggg acttattttt gcatagctga taatggaaat aagacagttg aagctcaggc
     2641 ggagcttact gtgcaagtgc cacctggatt cctgaaacaa cctgctaaca tatatgctca
     2701 cgaatccatg gacattgtat ttgaatgtga agtcactggg aagccaactc caactgtgaa
     2761 gtgggtcaag aatggggatg tggttatccc cagtgattac tttaaaattg taaaggaaca
     2821 taatcttcaa gttttgggtc tggtgaaatc agatgaaggg ttctatcaat gcattgctga
     2881 gaatgatgtt ggaaatgcac aagctggagc ccagctgata atccttgagc atgcaccagc
     2941 cacaacggga ccattacctt cagctcctcg agacgtcgtg gcctccctgg tctctactcg
     3001 cttcattaaa ttgacatggc gtacacctgc atcagaccct catggagaca atctcaccta
     3061 ctctgtgttc tacaccaagg aaggggttgc tagggagcgt gttgagaata ccagccagcc
     3121 aggagagatg caggtgacta ttcaaaactt gatgccagca actgtgtaca tcttcaaagt
     3181 tatggctcaa aataagcatg gctctggaga aagttcagct cctcttcgag tagagacaca
     3241 gcctgaggtt cagctccctg gcccagcacc taatatccgt gcttatgcaa cgtcacctac
     3301 ttctatcact gtcacctggg aaacaccgtt atctggcaat ggggaaattc aaaattacaa
     3361 attgtactac atggaaaaag gaactgataa agaacaggat attgatgttt caagtcactc
     3421 ctacaccatt aatggactga agaaatacac agaatacagt ttccgagtgg tggcctacaa
     3481 taaacatggt cctggagttt ctacacaaga tgttgctgtt cgaacattat cagatgttcc
     3541 cagtgctgct cctcagaatc tgtccttaga agtgagaaat tcaaagagta tagtgatcca
     3601 ctggcagccc ccttcctcaa ccacacaaaa tgggcagata actggctaca agattcgata
     3661 tcgaaaggcc tcccgaaaaa gtgatgtcac tgagaccttg gtaactggga cacagctgtc
     3721 tcagctgatt gaaggtcttg atcgggggac agaatataac ttccgagtcg ctgctctcac
     3781 agtcaatggt acaggtccag caactgattg gctgtctgct gaaacttttg aaagcgacct
     3841 agatgaaact cgtgttcctg aagtgcccag ctctcttcat gtccgtccgc tcgtcactag
     3901 cattgtagtg agctggactc ctccagagaa ccagaacatt gtggtccgag gttacgccat
     3961 cggttacggc attggcagcc ctcatgccca gaccatcaaa gtggactata aacaacgtta
     4021 ttacaccatc gaaaacttgg atccaagctc tcattacgtg attaccttga aagcatttaa
     4081 caatgttggc gaaggcatcc ccctttatga gagtgctgtg accagacctc acacagacac
     4141 ttctgaagtt gatttatttg ttattaatgc tccatacact ccagtgccag atcccactcc
     4201 catgatgcca ccagtgggag ttcaggcttc cattctgagt cacgacacca taaggattac
     4261 ctgggcagac aactccctgc ccaaacacca gaagattaca gactcccgct actacacagt
     4321 ccggtggaag accaacatcc cagcaaacac gaagtacaag aatgcaaatg caacgacgtt
     4381 aagctatttg gttactggtt taaagccaaa tacgctctat gagttctctg tgatggtgac
     4441 caaaggcaga aggtcaagca cgtggagtat gacagctcat ggcgctacct ttgaattagt
     4501 tcctacttct ccacctaagg atgtgacagt tgtgagtaag gaaggaaaac ctagaaccat
     4561 catagtgaac tggcagcctc cctctgaagc taacggcaag attacaggtt acatcatcta
     4621 ttacagcacg gatgtgaatg cagagataca tgactgggtt attgaaccag ttgtgggaaa
     4681 cagactgact caccagattc aagagttaac acttgatacg ccatactact tcaaaatcca
     4741 ggcccggaac tcaaagggca tggggcccat gtctgaagct gtacagttca gaacacctaa
     4801 agcggactcc tctgataaaa tgcctaatga ccaagcctta gggtcagcag gaaaaggaag
     4861 ccgactacca gacctgggat ctgactacaa acctccaatg agtggcagca acagccctca
     4921 cgggagcccc acctcccctc tggacagcac tagtggatct tccggaatca tcccagttga
     4981 ggaggagaac ccggacttct ggaaccgcga ggcagccgag gccctgggtg ccgccaagaa
     5041 gctgcagcct gcacagacag ccgccaagaa cctcatcatc ttcctgggcg atgggatggg
     5101 ggtgtctacg gtgacagctg ccaggatcct aaaagggcag aagaaggaca aactggggcc
     5161 tgagataccc ctggccatgg accgcttccc atatgtggct ctgtccaaga catacaatgt
     5221 agacaaacat gtgccagaca gtggagccac agccacggcc tacctgtgcg gggtcaaggg
     5281 caacttccag accattggct tgagtgcagc cgcccgcttt aaccagtgca acacgacacg
     5341 cggcaacgag gtcatctccg tgatgaatcg ggccaagaaa gcagggaagt cagtgggagt
     5401 ggtaaccacc acacgagtgc agcacgcctc gccagccggc acctacgccc acacggtgaa
     5461 ccgcaactgg tactcggacg ccgacgtgcc tgcctcggcc cgccaggagg ggtgccagga
     5521 catcgctacg cagctcatct ccaacatgga cattgacgtg atcctaggtg gaggccgaaa
     5581 gtacatgttt cgcatgggaa ccccagaccc tgagtaccca gatgactaca gccaaggtgg
     5641 gaccaggctg gacgggaaga atctggtgca ggaatggctg gcgaagcgcc agggtgcccg
     5701 gtatgtgtgg aaccgcactg agctcatgca ggcttccctg gacccgtctg tgacccatct
     5761 catgggtctc tttgagcctg gagacatgaa atacgagatc caccgagact ccacactgga
     5821 cccctccctg atggagatga cagaggctgc cctgcgcctg ctgagcagga acccccgcgg
     5881 cttcttcctc ttcgtggagg gtggtcgcat cgaccatggt catcatgaaa gcagggctta
     5941 ccgggcactg actgagacga tcatgttcga cgacgccatt gagagggcgg gccagctcac
     6001 cagcgaggag gacacgctga gcctcgtcac tgccgaccac tcccacgtct tctccttcgg
     6061 aggctacccc ctgcgaggga gctccatctt cgggctggcc cctggcaagg cccgggacag
     6121 gaaggcctac acggtcctcc tatacggaaa cggtccaggc tatgtgctca aggacggcgc
     6181 ccggccggat gttaccgaga gcgagagcgg gagccccgag tatcggcagc agtcagcagt
     6241 gcccctggac gaagagaccc acgcaggcga ggacgtggcg gtgttcgcgc gcggcccgca
     6301 ggcgcacctg gttcacggcg tgcaggagca gaccttcata gcgcacgtca tggccttcgc
     6361 cgcctgcctg gagccctaca ccgcctgcga cctggcgccc cccgccggca ccaccgacgc
     6421 cgcgcacccg ggtcatcatc accatcacca ttaatctaga gatcttatta aagcagaact
     6481 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     6541 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     6601 atgtctggtc gactctagct agactcttcc gcttcctcgc tcactgactc gctgcgctcg
     6661 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     6721 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     6781 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     6841 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     6901 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     6961 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     7021 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     7081 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     7141 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     7201 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     7261 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     7321 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     7381 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     7441 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     7501 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     7561 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     7621 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     7681 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     7741 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     7801 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     7861 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     7921 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     7981 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     8041 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     8101 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     8161 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     8221 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     8281 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     8341 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     8401 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     8461 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     8521 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     8581 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
     8641 gatgacggtg aaaacctctg acacatgcag ctcccggagg aatgtgtgtc agttagggtg
     8701 tggaaagtcc ccaggctccc caggcaggca gaagtatgca aagcatgcat ctcaattagt
     8761 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     8821 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     8881 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     8941 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     9001 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcgtcccg gagacggtca
     9061 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     9121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     9181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggaaat
     9241 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     9301 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     9361 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     9421 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     9481 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     9541 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     9601 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     9661 cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc cattcaggct gcgcaactgt
     9721 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     9781 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     9841 acggccagtg aatt
//
