LOCUS       sequence-13118-e        3861 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 3861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1609-HQ858762
FEATURES             Location/Qualifiers
     source          1..3861
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             112..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(1418..1517)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1534..1553)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1535..1553)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1558..1574)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1558..1574)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1676..1695)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1687..2496
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1754..1773)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2643..3231
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3132..3151
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3385..3402
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3561..3588)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3680..3766)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3815..3837
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3829..3846
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3830..3846
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc cttgtttaac
      121 tttaagaagg agcccttcac catgagcaga gacctcgagg acgggcacgg cgccgtcggc
      181 aacgttattg cggcagcagc agctgcagga ggggaggcgg tgggtgccgg cgaggccggg
      241 gacgcgcacg acaacgacgt ggtgatgccg gggttccggt tccaccccac ggaggaggag
      301 ctgatcgagt tctacctccg gcgcaaggtt gagggaaaac gcttcaacgt cgagctcatt
      361 gctttcctgg acctctaccg cttcgaccca tgggagcttc cagcaatggc ggtgatgggc
      421 gggaaggagt ggttcttcta cgtgccgagg gaccgtaagt accggaacgg agaccgccca
      481 aaccgggtga cggcgtcggg gtactggaag gcgacgggcg ctgaccggat gatccgaggc
      541 gagaacaacc gccccatcgg gctgaagaag acgctcgtgt tctactccgg caaggcgccc
      601 aagggcgtcc gcagcagctg gatcatgaac gagtaccgcc tcccgccacc acccaccgac
      661 gccgacccat tgattcccaa gtccgagatc tctctctgcc gcgtctacaa gcgctcaggc
      721 atcgacgacg gccacgggca gtcatcctcc agcacccaag cttcctcagc gcggcggcgg
      781 atctcctccc gcactggtgg tgtgccgacg acggccgttc agcacggatc gacgccgtca
      841 tcgatgccac tttcaccgac gccgcagcag ctcagcagct tccatccgct ccaaggagaa
      901 tgctcgtctg cgtcaccgcc ggagcccata atggatcaag ttgcggtcac cgtgcaccgt
      961 gcgtcgcctc agcttctccc tcaccgtcac cgcccgaggc cctgcccgta cgcgccggtg
     1021 atggcaacgg tatcagcagc ggaagctgcg ccgcaattgg cacacgcggc gactgcccac
     1081 gcctccacgt attcgctctt caacatggcc gctgccgccg ccgcgcctat ggccgccagc
     1141 tcaaggtcga ccattgacga gctgacaacg ctggttgctc ccacccaggc ctacgccaac
     1201 ctctcggccg cgacgacggg cggccacctc ctcccattac tgacgccgcc gccaccgcca
     1261 atgctacaaa tgacgccgct tggtgcgctg ccgatcgtgg cgccgccatt gtcgtcttca
     1321 gtcgctgaca agcttagcag ctgggactgg aacccgatac ctgacacgac agaccgggat
     1381 tacaatcctt ctggttttaa gaagggtggg cgcgccgacc cagctttctt gtacaaagtt
     1441 ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa
     1501 aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta ttacatggtc
     1561 atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa
     1621 gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg
     1681 ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc caacatggat
     1741 gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc
     1801 tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc
     1861 gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct
     1921 cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg
     1981 atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt
     2041 gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct
     2101 tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg
     2161 gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa
     2221 gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca
     2281 cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc
     2341 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct
     2401 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa
     2461 ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa
     2521 cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc aaaatccctt
     2581 aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     2641 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     2701 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     2761 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     2821 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     2881 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     2941 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     3001 acctacaccg aactgagata cctacagcgt gagcattgag aaagcgccac gcttcccgaa
     3061 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     3121 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     3181 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     3241 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     3301 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     3361 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     3421 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     3481 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac cgctagccag
     3541 gaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttagtttgat
     3601 gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt
     3661 tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga
     3721 taaaacgaaa ggcccagtct tccgactgag cctttcgttt tatttgatgc ctggcagttc
     3781 cctactctcg cgttaacgct agcatggatg ttttcccagt cacgacgttg taaaacgacg
     3841 gccagtctta agctcgggcc c
//
