LOCUS       sequence-131184-       10168 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the extracellular region of the PlexinA3 protein, 
            C-terminally fused to alkaline phosphatase + 6X histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10168)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 10168)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Plxna3-AP-His
FEATURES             Location/Qualifiers
     source          1..10168
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     CDS             6748..6765
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    6791..6912
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6828..6847)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6882..6901
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6950..6967)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7121..7709)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7201..7220)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7880..8671,8672..8740))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8503..8522
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8741..8845)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8913..8931
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8969..8991)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8993..9013)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8998..9328
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9179..9314
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9241..9260
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     9465..9484
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(9551..10006)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9688..9709)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9900..9919
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10132..10154
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10146..10163
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10147..10163
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tgcccactgt
     1621 ctgccttctc ccattgctat tcttcaccat aggagggtgc ctgggtagca gcaggccatt
     1681 tcgtaccttt gtggtaacag ataccactct gactcacctg gctgtgcacc gagtgactgg
     1741 ggaggtgttt gtaggtgccg tgaatcgagt tttcaagctg gctccaaacc taactgagct
     1801 acgggcccat gtcacagggc ccattgagga caatgctcgc tgctacccac ctcctagcat
     1861 gcgtgtgtgc tcccaccgcc tggtgcctgt ggacaatgtg aacaagctgc tcctcataga
     1921 ctatgcagcc cgtcgtttgg tagcttgtgg cagcatctgg cagggcatct gccagttcct
     1981 gcgtctggat gacctcttca agttgggtga gccccaccat cgcaaggagc actatctgtc
     2041 aggggcccag gagcctgatt ccatggctgg cgtcattgtt gaacaggtcc aggggcctag
     2101 caagttgttt gtgggcaccg ctgttgatgg caagtctgag tactttccca ccttgagttc
     2161 ccgtaagctc atcgacgatg aggacagtgg agatatgttc agtctggtgt accaggatga
     2221 gtttgtctct tctcagatca agatcccttc agacacattg tccttgtacc ctgcctttga
     2281 catctactac atatatggct ttgtcagtgc ctccttcgtg tacttcttaa cgttgcagct
     2341 ggacacccag cagacgctgt tggatacagc aggcgagaaa ttcttcacat ccaagatcgt
     2401 gcgcatgtgt gcaggggact cagagttcta ctcttatgta gagttcccta ttggctgctc
     2461 ctggcgtggt gtggagtacc gcttggtgca gagtgcccat ctggccaagc caggcctgct
     2521 attggcccag gctctgggtg tgccagccga cgaggatgtc ctcttcacca tcttctctca
     2581 gggccagaag aacagagcca acccacctcg gcagaccatc ctttgccttt tcaccctcag
     2641 cagcattaat gcccacatcc ggcgccgaat ccaatcgtgc taccgtggag agggcacgct
     2701 ggccctaccc tggctactga ataaggagct gccctgcatc aacacccctc tgcagatcaa
     2761 tggaaacttc tgtgggttag tgttgaatca accattgggt ggcctacacg tgattgaggg
     2821 gctacccttg ctggctgaca gcactgatgg catggccagt gtggctgcct acacctacca
     2881 ccagcattct gtggttttca tcggtacacg cagcggtaat ttgaagaagg tgcgggtcga
     2941 tggctctcag gatgcccagc tatatgaaac ggtctctgtg gtgcagggca gccccatact
     3001 tcgagacctg ctcttcagcc ctgaccaccg acacatctac ctcctgagtg agaagcaggt
     3061 gagccaactc ccggtggaga cctgtgagca gtatctgagc tgtgctgcat gcctgggctc
     3121 aggggaccca cactgtggtt ggtgtgtgct acagcacagg tgctgccgtg aaggggcctg
     3181 tccaggtgcc tctgccccac atggctttgc agaagagctg agcaaatgta tacaggtgcg
     3241 ggtccgtccc aataatgtgt cagtgacatc ctctggggtg cagctgaccg tagccatgcg
     3301 caacgtgcca gacctcagtg tgggtgtgag ctgttccttt gaggaggtga ctgaaagtga
     3361 ggctattctg ctgccctctg gagagttacg atgtccttca ccatcccttc aggagctcca
     3421 gacacttacc agagggcatg gggccactca tactgtgcgg cttcagctgc tctccatgga
     3481 gactggtgtg aggtttgctg gggttgactt tgtcttctac aactgcagtg ccctccagtc
     3541 gtgtatgtcc tgtgttggca gcccttaccc ctgccactgg tgtaagtacc gtcatgtgtg
     3601 taccagccac ccacacgagt gctctttcca ggagggcagg gtccacagcc ctgagggctg
     3661 ccctgagatc ctgcctcaag gggacctctt gattcctgtg ggtgtcatgc agcctctaac
     3721 tctgcgggct aagaacctgc cacagcctca gtccggacag aagaattatg aatgcgtggt
     3781 ccgtgtacag ggacggcaac atcgggtacc tgcagtgcgc tttaacagca gcagtgtgca
     3841 gtgccagaat gcctcgtact tctatgaagg tgatgagttt ggtgacaccg aactggactt
     3901 ctctgtggtt tgggatggag atttccccat tgataagcct cctagcttcc gagcccttct
     3961 ttacaagtgc tgggctcagc ggcctagctg tggcctctgc ctcaaggctg atccccgatt
     4021 caactgtggc tggtgcatct cagagcacag gtgccagctg agggctcact gcccagctcc
     4081 caaaagtaac tggatgcacc caagccagaa gggtgcccga tgcagccatc cccgaatcac
     4141 ccagattcat ccacttacag gacccaagga gggtggcacc cgggtcacca ttgtgggtga
     4201 gaacctgggc ctcacttccc gtgaggttgg ccttcgagta gctggtgtac gttgcaactc
     4261 cattcccacc gaatatgtca gtgctgaaag gatcgtatgt gagatggagg aatcgctggt
     4321 gcccagccca ccacctgggc ctgctgagct ctgtgtaggg gattgttctg ctgacttccg
     4381 cacacagtcc cagcaactct acagctttgt gacccctaca tttgaccgtg tgagtccctc
     4441 tcggggccca gcttctggag gcactcggct taccatctct ggaatttctc tggatgccgg
     4501 cagcagggtc acagtgatta taagagatgg cgagtgccag tttgtgagga gagatgccga
     4561 ggcaatcgtg tgtatctcac ctgtctccac cctgggcccc agtcagagcc ctatcaccct
     4621 tgccatcgat catgccaaca tctccaacac tggagtcatc tatacctaca cccaggaccc
     4681 tacggtcaca caccttgagc ccacctggag catcatcaat ggaagcacct ccatcactgt
     4741 gagtggaacc catctgctga cagttcaaga accccgtgta cgagccaagt accgtggtat
     4801 tgagaccact aacacatgcc aggtgatcaa cgacactgca atgctatgta aggcccctgg
     4861 catcttcctt gggcaccctc agcctcgggc ccaaggcgag caccctgacg agtttggctt
     4921 cttgctggac cacgtgcagg cagcccgctc cctcaaccgt tcttccttca cctactaccc
     4981 tgatcccagc tttgaaccac ttgggccctc tggtgtgcta gatgtcaaac ctggttcaca
     5041 tgttgtattg aagggcaaga acctgatccc tgctgcagct ggcagctccc gcctcaacta
     5101 cacagtgttg attggaggac agccatgtgc actaactgtc tcagatactc aacttctgtg
     5161 tgattcccca agccagacag gccggcagcc tgttatggtg ctggtgggtg gcctggagtt
     5221 ctggttgggc acgctgcaca tcactgctga tcgggcactg acactagtgg atcttccgga
     5281 atcatcccag ttgaggagga gaacccggac ttctggaacc gcgaggcagc cgaggccctg
     5341 ggtgccgcca agaagctgca gcctgcacag acagccgcca agaacctcat catcttcctg
     5401 ggcgatggga tgggggtgtc tacggtgaca gctgccagga tcctaaaagg gcagaagaag
     5461 gacaaactgg ggcctgagat acccctggcc atggaccgct tcccatatgt ggctctgtcc
     5521 aagacataca atgtagacaa acatgtgcca gacagtggag ccacagccac ggcctacctg
     5581 tgcggggtca agggcaactt ccagaccatt ggcttgagtg cagccgcccg ctttaaccag
     5641 tgcaacacga cacgcggcaa cgaggtcatc tccgtgatga atcgggccaa gaaagcaggg
     5701 aagtcagtgg gagtggtaac caccacacga gtgcagcacg cctcgccagc cggcacctac
     5761 gcccacacgg tgaaccgcaa ctggtactcg gacgccgacg tgcctgcctc ggcccgccag
     5821 gaggggtgcc aggacatcgc tacgcagctc atctccaaca tggacattga cgtgatccta
     5881 ggtggaggcc gaaagtacat gtttcgcatg ggaaccccag accctgagta cccagatgac
     5941 tacagccaag gtgggaccag gctggacggg aagaatctgg tgcaggaatg gctggcgaag
     6001 cgccagggtg cccggtatgt gtggaaccgc actgagctca tgcaggcttc cctggacccg
     6061 tctgtgaccc atctcatggg tctctttgag cctggagaca tgaaatacga gatccaccga
     6121 gactccacac tggacccctc cctgatggag atgacagagg ctgccctgcg cctgctgagc
     6181 aggaaccccc gcggcttctt cctcttcgtg gagggtggtc gcatcgacca tggtcatcat
     6241 gaaagcaggg cttaccgggc actgactgag acgatcatgt tcgacgacgc cattgagagg
     6301 gcgggccagc tcaccagcga ggaggacacg ctgagcctcg tcactgccga ccactcccac
     6361 gtcttctcct tcggaggcta ccccctgcga gggagctcca tcttcgggct ggcccctggc
     6421 aaggcccggg acaggaaggc ctacacggtc ctcctatacg gaaacggtcc aggctatgtg
     6481 ctcaaggacg gcgcccggcc ggatgttacc gagagcgaga gcgggagccc cgagtatcgg
     6541 cagcagtcag cagtgcccct ggacgaagag acccacgcag gcgaggacgt ggcggtgttc
     6601 gcgcgcggcc cgcaggcgca cctggttcac ggcgtgcagg agcagacctt catagcgcac
     6661 gtcatggcct tcgccgcctg cctggagccc tacaccgcct gcgacctggc gccccccgcc
     6721 ggcaccaccg acgccgcgca cccgggtcat catcaccatc accattaatc tagagatctt
     6781 attaaagcag aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     6841 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     6901 caatgtatct tatcatgtct ggtcgactct agctagactc ttccgcttcc tcgctcactg
     6961 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     7021 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     7081 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     7141 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     7201 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     7261 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     7321 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     7381 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     7441 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     7501 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     7561 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     7621 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     7681 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     7741 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     7801 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     7861 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     7921 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     7981 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     8041 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     8101 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     8161 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     8221 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     8281 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     8341 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     8401 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     8461 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     8521 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     8581 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     8641 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     8701 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     8761 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     8821 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
     8881 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
     8941 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gaggaatgtg
     9001 tgtcagttag ggtgtggaaa gtccccaggc tccccaggca ggcagaagta tgcaaagcat
     9061 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     9121 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     9181 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     9241 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     9301 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgt
     9361 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     9421 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     9481 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     9541 ccgcatcagg aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     9601 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
     9661 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     9721 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
     9781 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
     9841 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     9901 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
     9961 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccat tcgccattca
    10021 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
    10081 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
    10141 gacgttgtaa aacgacggcc agtgaatt
//
