LOCUS       sequence-13119-e        3804 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 3804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1663-HQ858763
FEATURES             Location/Qualifiers
     source          1..3804
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             112..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(1361..1460)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1477..1496)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1478..1496)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1501..1517)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1501..1517)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1619..1638)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1630..2439
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1697..1716)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2586..3174
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3075..3094
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3328..3345
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3504..3531)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3623..3709)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3758..3780
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3772..3789
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3773..3789
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc cttgtttaac
      121 tttaagaagg agcccttcac catgtcggcc gccgaaaccg ctgtttctcc gacgacgacg
      181 ccgcagccgc agctgcaatc ggatccgaac gccgccgccg acgcggccag ttccaattct
      241 ctctgggtgg ccgacgagac gcaaacccta accgaggtcc tgctccaacc ccaaccaaac
      301 cacctgtcgc ctcctccttc tggtgaaggg gacgacggga tcactgcctc cggtgctcct
      361 gttggtgggg acgccgcggc atctgcttct gctgcggcgg cggctaacgc gacgatagaa
      421 gagcgggtga gggggccgtg gtccccagag gaggacgcgg tgctgagcaa cctcgtggag
      481 aagttcgggg cgcggaactg gacgctgatc gcgcgtggca tcccgggccg ctccggcaag
      541 tcctgccgcc tgcgctggtg caatcagctc gacccccaag tcaagcgcaa gcctttcact
      601 gaagaggagg accgcattat aatggcagct catgctgtcc atgggaacaa gtgggcatct
      661 atcgcaaagc ttttggatgg gagaacagac aatgccatca agaatcactg gaattccacc
      721 ctgaggcgtc gctactgcaa tgatggccaa tgcaagcatg gtggttctgt ggagcgaagt
      781 atcccaaagg tgtccaggga agtttcagaa gaaccttggc ctttaaacga tcttaattcc
      841 ttcaccgcta tggatgtaaa ggatgatcct gtgcaaacag ctccagatac ttctcctcgg
      901 gcaaggcaca tcacagatca ttgttctatt actcaagtcg ttgatccacc atacctgtcc
      961 cgaccagcgg caaagattgg tgctttcaga ccatattatc taggacacac ggaacctacc
     1021 caacaagaaa tgccaggtgc tgtcgtcatg tttgactcaa ctcataaagt ctcgactcct
     1081 gaaagcgagg tattcaagtt tgttgatcct atatgctttg ctgctgaggt gccagacaaa
     1141 tgtgggtatg gttgctgcgg tgctcatgag cagcatcgta agaactcatt gctgggtcct
     1201 gagttcagtg agtttgaagg tcatccacct gttttaaata ttagctttgc ttcattggta
     1261 tcagaaatta gcagcattgc ctggatgaga agtggtatgc aaagcagtga ttccagcaac
     1321 ttgatgcaat caattccacc aaccaagggt gggcgcgccg acccagcttt cttgtacaaa
     1381 gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt
     1441 caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc gtattacatg
     1501 gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca
     1561 caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca
     1621 aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg
     1681 gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca
     1741 atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt
     1801 agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg
     1861 cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact
     1921 gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat
     1981 attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt
     2041 ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt
     2101 ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg
     2161 aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca tggtgatttc
     2221 tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga
     2281 gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt
     2341 tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat
     2401 aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt taattggttg
     2461 taacactggc agagcattac gctgacttga cgggacggcg caagctcatg accaaaatcc
     2521 cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     2581 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     2641 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     2701 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     2761 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     2821 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2881 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2941 acgacctaca ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc
     3001 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     3061 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     3121 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     3181 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     3241 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     3301 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     3361 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     3421 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc
     3481 caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt
     3541 gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa
     3601 cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac
     3661 agataaaacg aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag
     3721 ttccctactc tcgcgttaac gctagcatgg atgttttccc agtcacgacg ttgtaaaacg
     3781 acggccagtc ttaagctcgg gccc
//
