LOCUS       sequence-13120-e        3429 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1680-HQ858764
FEATURES             Location/Qualifiers
     source          1..3429
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             112..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(986..1085)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1102..1121)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1103..1121)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1126..1142)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1126..1142)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1244..1263)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1255..2064
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1322..1341)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2211..2799
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2700..2719
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2953..2970
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3129..3156)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3248..3334)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3383..3405
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3397..3414
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3398..3414
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc cttgtttaac
      121 tttaagaagg agcccttcac catgttgccg taccctaaca accctcacca tttcggcgtc
      181 tcccaagaac tcccacaccc aaaccctacg gccggcttcc ccatgcctcc accgggcgcg
      241 catctggatc agcgctacta cgtggaccat cacttctttc ccggccacgg tcactgcttc
      301 aactccgaga ctctggaggc ggtgctgagg ccggcgcgcg ctgccccaga gtgcgacgag
      361 gccctagtag tcacggcgcc gcaggcgggt ggaagaagaa acggtagtct gcctgccgcc
      421 gccgccgccg gccagggcca gggccagggc cacgcccgag cgcggaagcg cccgttccgg
      481 acggaccggc acagcaagat ccggacggcg cagggcgtgc gcgaccgccg gatgcggctg
      541 tccctcgacg tcgcccgcga cttcttcgct ctgcaggacc ggctcggctt cgataaggcc
      601 agcaagacgg tggactggct gctcacccag tccaagccgg ccatcgagcg gctcgccgcc
      661 gccaccgagc cctcccacca gcggagcgac gacgcagctt tctcaccccc aacgtcaggg
      721 gtggctgatg tgtcagctgg taataagagc ggaggagttg ccgagaagtt gggccgctcc
      781 agaagtgggg gatccgcgtc catggagatg gagcgcgcct gcgggctgga ccgcctcgtg
      841 tcggccgcac cagtgcttcg tgagtactac tacggcctca gcgagatgat gagcaacgac
      901 aacggaggag atggcgacga cgacggtgag tacgaggaag acggtgattt cctggacggt
      961 atgcaatata agggtgggcg cgccgaccca gctttcttgt acaaagttgg cattataaga
     1021 aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt
     1081 atttgccatc cagctgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc
     1141 tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata
     1201 tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc
     1261 catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc tgatttatat
     1321 gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgcttgtat
     1381 gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat
     1441 gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc
     1501 aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat ccccggaaaa
     1561 acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg
     1621 gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat
     1681 cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt
     1741 gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa
     1801 cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt
     1861 atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac
     1921 cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag
     1981 aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat
     2041 ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc
     2101 attacgctga cttgacggga cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac
     2161 gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     2221 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     2281 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     2341 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     2401 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     2461 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     2521 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     2581 ctgagatacc tacagcgtga gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg
     2641 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     2701 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     2761 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     2821 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     2881 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     2941 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     3001 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     3061 aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga agagtttgta
     3121 gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttt
     3181 atggcgggcg tcctgcccgc caccctccgg gccgttgctt cacaacgttc aaatccgctc
     3241 ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg
     3301 cccagtcttc cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgcg
     3361 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaag
     3421 ctcgggccc
//
