LOCUS       sequence-131208-        8750 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the Sema3F protein (truncated at cleavage site P3; ie, 
            long and contains a 93 nt deletion in exon 3), C-terminally fused to
            alkaline phosphatase + 6X histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8750)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 8750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Sema3f(L,-)-AP-His
FEATURES             Location/Qualifiers
     source          1..8750
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     CDS             5330..5347
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    5373..5494
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5410..5429)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5464..5483
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5532..5549)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5703..6291)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5783..5802)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6462..7253,7254..7322))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7085..7104
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7323..7427)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7495..7513
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7551..7573)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7575..7595)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7580..7910
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7761..7896
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7823..7842
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     8047..8066
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(8133..8588)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8270..8291)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8482..8501
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8714..8736
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8728..8745
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8729..8745
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tgcttgtcac
     1621 tgccttcatc ctctgggctt ccctgctgac cggggcctgg cctgctaccc ctatccagga
     1681 ccagcttcca gccacaccta gagtccgact gtcattcaaa gaacttaagg ccaccggtac
     1741 cgcccacttc ttcaactttc tgctcaacac tacagactac agaatcctgc tcaaggatga
     1801 ggaccatgac cgcatgtatg tgggcagcaa ggactacgtg ctgtccctgg acctgcatga
     1861 catcaaccga gagcccctta ttatccattg ggcagcctcc ccgcagcgca ttgaggagtg
     1921 catactgtca ggcaaggatg gcaatggaga gtgtggtaac ttcgtccggc tcatccagcc
     1981 ttggaaccga acacacctgt atgtgtgtgg gaccggtgcc tacaacccca tgtgcaccta
     2041 tgtgaaccgt ggccgtcgcg cacaggatta catcttctac ctggagcctg agaaactgga
     2101 gtcaggaaaa ggcaaatgtc cctatgaccc caagctggac acagcctcag ccctcatcaa
     2161 tgaggaactc tatgcaggag tgtacatcga ttttatgggt accgatgcag ccattttccg
     2221 cacgctgggg aagcagacag ccatgcgcac agatcagtac aactcccggt ggctcaatga
     2281 tccttccttc atacatgctg agctcatccc tgacagcgca gagcgcaatg acgataaact
     2341 ctacttcttc ttccgagagc gctcggcaga ggcgccacag aaccctgctg tgtatgcccg
     2401 catcgggcgc atctgcctca acgatgacgg tggccattgc tgcctggtca acaagtggag
     2461 cacattcctg aaggctcggc tcgtctgctc cgtgccaggc gaggatggta tcgagaccca
     2521 cttcgacgaa ctccaggatg tgtttgtcca gcagacccag gacatcagga acccagtcat
     2581 ttatgctgtc tttacctctt caggctctgt gttccgaggg tctgcagtgt gtgtctactc
     2641 catggctgat atccgcatgg tcttcaatgg gccttttgct cacaaggagg gccccaacta
     2701 tcaatggatg cccttctcag gaaagatgcc ctatcctcgg cctggcacat gccctggtgg
     2761 aaccttcacg ccctcgatga agtccactaa ggactaccct gatgaagtga tcaacttcat
     2821 gcgcactcat ccgctcatgt accaggccgt ctacccgctc cagcggcgac ccctggtggt
     2881 ccgtacaggc gctccctacc gcctcaccac tgtcgctgtg gaccaggtgg atgcagctga
     2941 tggacgctat gaggtgcttt tcctgggcac agaccgcggg acagtgcaga aggtcatcgt
     3001 gctgcccaag gacgaccagg aggtggaaga gctgatgcta gaggaagtgg aggtcttcaa
     3061 ggaaccagca cctgttaaga ccatgaccat ctcttccaag aggcaacaac tgtatgtggc
     3121 ctcagccgtg ggtgtcacac acctgagcct gcaccgctgc caggcctatg gagctgcctg
     3181 tgccgactgc tgcctcgccc gcgatcccta ctgtgcctgg gatggccagg cctgctctcg
     3241 ctacacagca tcctccaaga ggcggagtcg ccggcaagat gtccgtcacg ggaaccctat
     3301 cagacagtgc cgtggtttca attccaatgc aaacaagaat gccgtagagt ctgtgcagta
     3361 cggagtggcc ggcagcgcag ctttccttga gtgtcagccc cgctcgcctc aagccactgt
     3421 caagtggctg ttccagcgag atcccagtga tcggcgccgt gagattcgcg cagaggaccg
     3481 cttcctgcgt acagagcagg ggctgttgct tcgcgccctg cagcttggtg accgcggcct
     3541 ctactcctgc acggccactg agaacaactt caagcacatc gtcacgcggg tgcagctgca
     3601 tgtactgggc cgggacgccg tccatgctgc cctcttcccc ccactggctg tgagcgtccc
     3661 tccacctcct ggcacaggcc ctccaacgcc tccttaccag gagctggccc agctcctagc
     3721 ccagccggaa gtgggcctca tccatcagta ctgccagggt tactggcgcc acgtgccccc
     3781 cagccccagg gaggcgccag gagcacttag gcctcctgaa ctccaggacc agaaaaagcc
     3841 gggcgcgcct ggatcttccg gaatcatccc agttgaggag gagaacccgg acttctggaa
     3901 ccgcgaggca gccgaggccc tgggtgccgc caagaagctg cagcctgcac agacagccgc
     3961 caagaacctc atcatcttcc tgggcgatgg gatgggggtg tctacggtga cagctgccag
     4021 gatcctaaaa gggcagaaga aggacaaact ggggcctgag atacccctgg ccatggaccg
     4081 cttcccatat gtggctctgt ccaagacata caatgtagac aaacatgtgc cagacagtgg
     4141 agccacagcc acggcctacc tgtgcggggt caagggcaac ttccagacca ttggcttgag
     4201 tgcagccgcc cgctttaacc agtgcaacac gacacgcggc aacgaggtca tctccgtgat
     4261 gaatcgggcc aagaaagcag ggaagtcagt gggagtggta accaccacac gagtgcagca
     4321 cgcctcgcca gccggcacct acgcccacac ggtgaaccgc aactggtact cggacgccga
     4381 cgtgcctgcc tcggcccgcc aggaggggtg ccaggacatc gctacgcagc tcatctccaa
     4441 catggacatt gacgtgatcc taggtggggg ccgaaagtac atgtttcgca tgggaacccc
     4501 agaccctgag tacccagatg actacagcca aggtgggacc aggctggacg ggaagaatct
     4561 ggtgcaggaa tggctggcga agcgccaggg tgcccggtac gtgtggaacc gcactgagct
     4621 catgcaggct tccctggacc cgtctgtggc ccatctcatg ggtctctttg agcctggaga
     4681 catgaaatac gagatccacc gagactccac actggacccc tccctgatgg agatgacaga
     4741 ggctgccctg cgcctgctga gcaggaaccc ccgcggcttc ttcctcttcg tggagggtgg
     4801 tcgcatcgac catggtcatc atgaaagcag ggcttaccgg gcactgactg agacgatcat
     4861 gttcgacgac gccattgaga gggcgggcca gctcaccagc gaggaggaca cgctgagcct
     4921 cgtcactgcc gaccactccc acgtcttctc cttcggaggc taccccctgc gagggagctc
     4981 catcttcggg ctggcccctg gcaaggcccg ggacaggaag gcctacacgg tcctcctata
     5041 cggaaacggt ccaggctatg tgctcaagga cggcgcccgg ccggatgtta ccgagagcga
     5101 gagcgggagc cccgagtatc ggcagcagtc agcagtgccc ctggacgaag agacccacgc
     5161 aggcgaggac gtggcggtgt tcgcgcgcgg cccgcaggcg cacctggttc acggcgtgca
     5221 ggagcagacc ttcatagcgc acgtcatggc cttcgccgcc tgcctggagc cctacaccgc
     5281 ctgcgacctg gcgccccccg ccggcaccac cgacgccgcg cacccgggtc atcatcacca
     5341 tcaccattaa tctagagatc ttattaaagc agaacttgtt tattgcagct tataatggtt
     5401 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     5461 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggtcgact ctagctagac
     5521 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     5581 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     5641 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     5701 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     5761 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     5821 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     5881 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     5941 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     6001 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     6061 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     6121 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     6181 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     6241 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     6301 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     6361 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     6421 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     6481 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     6541 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     6601 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     6661 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     6721 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     6781 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     6841 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     6901 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     6961 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     7021 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     7081 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     7141 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     7201 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     7261 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     7321 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     7381 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     7441 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     7501 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     7561 atgcagctcc cggaggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagg
     7621 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     7681 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     7741 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     7801 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     7861 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     7921 gagcttgtat atccattttc gtcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     7981 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
     8041 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
     8101 acagatgcgt aaggagaaaa taccgcatca ggaaattgta agcgttaata ttttgttaaa
     8161 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     8221 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
     8281 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     8341 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
     8401 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     8461 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
     8521 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     8581 gggcgcgtcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
     8641 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
     8701 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt
//
