LOCUS       sequence-13121-e        4413 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4413)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 4413)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1681-HQ858765
FEATURES             Location/Qualifiers
     source          1..4413
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             112..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(1970..2069)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2086..2105)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2087..2105)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2110..2126)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2110..2126)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2228..2247)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2239..3048
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2306..2325)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3195..3783
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3684..3703
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3937..3954
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4113..4140)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4232..4318)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4367..4389
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4381..4398
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4382..4398
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc cttgtttaac
      121 tttaagaagg agcccttcac catggcggga gatccggccg ccggggtcgg cggcaacgcc
      181 caccccgcgt cggggagcaa gagctcccgc tcgtcgacgc ggcaccgtca gttccgcgac
      241 cgagccaagg tccgagtcga cgacctacaa gagatgttct gcggcctcca atccgctcgc
      301 aaggagagcc gctccgccga cgccgctgtc ctcgaggagc aggtccacca gatgctccgc
      361 gagtggcgtg ccgagctcag cgtcccatcc ccggcttcct ctctccagaa ctcgcagggg
      421 aacaaccggg aggcttcgga tccaccgtcg gatacgctgc gactgctgca gctggcggtg
      481 cccgaggagg aggacgatgc cagcagtaag ctcctggtgc ccccttctcg tcagacccac
      541 gagcacgggc aggacagcag gatggccaat actgatcagc agtgtgaagc tatagatggg
      601 ggcactgctc ctgcgcagca ctctctaggt cagggagtgg atggagattg tggagaagtg
      661 tcttctgttg ctaatgcaat gttcaatcat cggatgtact atacagacta tgaacttaat
      721 attgatgatt ttctgtgtgg tgatggttac aaaataaata tttctggttc taatgaagat
      781 cagttcaata atctgcatgg gattggccag ttgggacatc aacagtttga tttgccatta
      841 gatttgccac ctactcattc atatgctgat gcaaataact ctgagcaaaa tactggagat
      901 gtttttgttc acatgtcaga cttgcttaca acaatatggc cgtcaccctc tcagtatttg
      961 gggccaaaat gtgcactttg ggactgtggt aggccagctg gagggacaga agactctgga
     1021 gattactgca acccttacca tgctggtttg gctttgaatg atgatggtct tcttgggata
     1081 agacctgtga tgcgtccaag aggtattgat ttgaaagatg ggccactatt tgctgctctc
     1141 attgcaaaag ttcaaggaaa gaacgttggt attcctgtgt gtggaggcgc tgctacgtct
     1201 aaatccccat ggaatgcacc tgaacttttc gatctttctc ttctggaggg tgaatctctt
     1261 agagaatggt tgttctttga tactccaaga agagcatttg agagtggcaa ccggaagcag
     1321 aggtcattgc cggattacgg tggtcgtggg tggcatgaat caaggaagct ggtaatgaaa
     1381 gatttcgcag gtctgaagag gtcctattac atggacccac aaccatcaag cagccatgag
     1441 tggcatcttt tcgagtatga gatcaatgct tctgatgccc tcgccttgta ccgtcttgag
     1501 tacaagtcct ctgaccccaa aaagggtgct gctaagtcaa aactggctag tccactgaat
     1561 gaaatccggc agcgaatggg caggctgact gcagacagcc ctgtggaaaa caagcggact
     1621 gctaggagca agccaaaggc taaccagaac cagaaggata ccaatgcaaa tacccgtcca
     1681 catgttaaca ctcccaatca agttaatgct ccaaatgctt atcagacaac gcagcaggtg
     1741 aaccaaatgg catttctgaa tggaaacgtt gtttatggac ctcaccttcc acattctgaa
     1801 agcgtcgtct atggacctca tcttccacat tctgaaaacg ttgtctacgg acctcatctt
     1861 ccacattctg aaaacgtcgt ctatggacct caccttccac agggatactc agccgaagga
     1921 agcagcttct tttggaaccc aagtgacggg actaagggtg ggcgcgccga cccagctttc
     1981 ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc
     2041 actatcagtc aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg
     2101 tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt
     2161 tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc ttacataaac
     2221 agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat
     2281 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca
     2341 ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat
     2401 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg
     2461 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta
     2521 ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata tcctgattca
     2581 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt
     2641 tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg
     2701 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa
     2761 caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat
     2821 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat
     2881 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc
     2941 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct
     3001 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt
     3061 aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgc aagctcatga
     3121 ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag
     3181 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     3241 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     3301 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     3361 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     3421 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     3481 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     3541 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagcattg agaaagcgcc
     3601 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     3661 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     3721 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     3781 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     3841 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     3901 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     3961 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     4021 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt
     4081 accgctagcc aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct
     4141 gcttagtttg atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt
     4201 gcttcacaac gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg
     4261 acaaacaaca gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat
     4321 gcctggcagt tccctactct cgcgttaacg ctagcatgga tgttttccca gtcacgacgt
     4381 tgtaaaacga cggccagtct taagctcggg ccc
//
